Fig. 1 | The ISME Journal

Fig. 1

From: A modular atomic force microscopy approach reveals a large range of hydrophobic adhesion forces among bacterial members of the leaf microbiota

Fig. 1

FluidFM-based single-cell force spectroscopy using functionalized beads. a Schematic representation of the experimental setup. A functionalized silica bead is reversibly immobilized by application of a negative pressure through the microchanneled cantilever. The bead is used to probe for interactions with bacteria that are immobilized on polydopamine-coated glass. A laser beam reflected on the back of the cantilever serves to monitor the forces acting on the cantilever. The feedback control between the photodetector and the piezo element allows precise application of defined forces as well as detection of adhesion forces. b Workflow of adhesion measurements. The cantilever is deflected due to the forces acting between bacterium and bead over the time of the experiment. Adhesion forces (Fadh) were derived from the maximally measured force during the retraction phase. After each measurement the immobilized bead is used again for another cell or exchanged