Fig. 2 | The ISME Journal

Fig. 2

From: Elucidation of glutamine lipid biosynthesis in marine bacteria reveals its importance under phosphorus deplete growth in Rhodobacteraceae

Fig. 2

a Proposed pathway for the biosynthesis of glutamine lipid, in comparison to ornithine lipid biosynthesis, in R. pomeroyi DSS-3. The first step is carried out by an N-acetyltransferase encoded by glsB and olsB, respectively and lysolipid intermediates are formed. The second step is mediated by an O-acetyltransferase encoded by olsA by esterification of a second fatty acid to the hydroxyl group of the lysolipid intermediates. b The gene neighbourhood of olsB (SPO1980) and glsB (SPO2489) in the genome of R. pomeroyi DSS-3. c Extracted ion chromatograms (EIC) obtained after analysing lipid extract from the wild-type R. pomeroyi DSS-3 and the olsA and glsB mutants by mass spectrometry in negative ion mode. Ions with mass-to-charge (m/z) 717 correspond to the intact mass of the glutamine lipid (Fig. 1)

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