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Ischemic postconditioning reduces spinal cord ischemia-reperfusion injury through ATP-sensitive potassium channel

Abstract

Study design

Animal study.

Objectives

Explore the neuroprotective effect of remote limb ischemic postconditioning (Post C) in spinal cord ischemic reperfusion injury (SCII) and related mechanisms.

Setting

Anesthesiology Laboratory of Southwest Medical University.

Methods

We established a rabbit SCII model and processed it with Post C. To evaluate the neural function, spinal cord tissue was taken 48 h later, normal neurons were evaluated by HE staining, and the expression of ATP-sensitive potassium channel (KATP) marker molecule Kir6.2 was detected by Western blot. Immunofluorescence detection of spinal cord Iba-1 expression, ELISA detection of M1 type microglia marker iNOS and M2 type microglia marker Arg, and Western blot detection of NF-κB and IL-1β expression. Through these experiments, we will explore the protective effect of Post C in SCII, observe the changes in the protective effect after using KATP blockers, and verify that Post C can play a neuroprotective effect in SCII by activating KATP.

Results

We observed that Post C significantly improved exercise ability and the number of spinal motor neurons in the SCII model. Microglia are activated and expression of M1 microglia in the spinal cord was decreased, while M2 was increased. This neuroprotective effect was reversed by the nonspecific KATP inhibitor.

Conclusion

Post C has a neuroprotective effect on SCII, and maybe a protective effect produced by activating KATP to regulate spinal microglia polarization and improve neuroinflammation.

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Fig. 1
Fig. 2: Activation of microglia in the spinal cord.
Fig. 3

Data availability

Raw data related to the paper can be obtained by email from the corresponding author.

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Acknowledgements

Thanks to Dr. Jianguo Feng from the Anesthesiology Laboratory of Southwest Medical University for supporting this study. This study was supported by Zigong Science and Technology Bureau (2021YLSF16).

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Authors and Affiliations

Authors

Contributions

JF designed the experiment and carried out the experiment; GM was involved in experiment design, experiment implementation, paper writing, data management; XL and CO participated in the implementation of part of the experiment, while JZ was responsible for the whole experiment and the final authorization.

Corresponding authors

Correspondence to Guo Mu or Jiaomei Zhao.

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Competing interests

The authors declare no competing interests.

Ethical approval

This study was approved by the Animal Ethics Committee of Southwest Medical University.

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Fu, J., Mu, G., Liu, X. et al. Ischemic postconditioning reduces spinal cord ischemia-reperfusion injury through ATP-sensitive potassium channel. Spinal Cord 60, 326–331 (2022). https://doi.org/10.1038/s41393-021-00714-5

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