Chemogenetic activation of G12 signaling enhances adipose tissue browning

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Beige adipocytes, which increase energy expenditure by dissipating energy as heat, have gained attention as a therapeutic target for combating obesity. 1 Adipocytes express many types of G-protein-coupled receptors (GPCRs), each of which has a unique preference for the G s , G i , G q , and G 12 subfamilies.While the function of G s -coupled β-adrenergic receptors in beige adipocyte induction is well established, 2 little is known about the function of G 12 -coupled GPCRs beyond its suppressive roles in white adipocyte maturation. 3In this study, we generated transgenic mice conditionally expressing a G 12 -coupled designer GPCR using a Cre-loxP system and investigated the potential effects of G 12 signaling on adipocyte biology.
We first sought to improve the G 12 -coupling selectivity of the previously established G 12 -coupled Designed Receptor Exclusively Activated by Designer Drugs (DREADD; M3D-GPR183/ICL3), which showed a leaky coupling to other G-protein subtypes such as G o . 4sing PRECOG, 5 a GPCR-coupling prediction algorithm, we designed six single-point mutants and assessed G-proteincoupling activity upon stimulation with the designer ligand, clozapine N-oxide (CNO) (Supplementary Fig. 1a).The F 1.57 V mutant (superscript denotes Ballesteros-Weinstein numbering) reduced off-target G o coupling while maintaining G 12 coupling and a surface expression level (Fig. 1a, Supplementary Fig. 1b).Gprotein-coupling profiling of the F 1.57 V construct revealed preferential activation of G 12 among the four G-protein subfamilies (Fig. 1b).We chose the F 1.57 V mutant of the M3D-GPR183/ ICL3 construct (hereafter referred to as G 12 D) for the following transgenic mouse study.
We generated mice expressing HA epitope-tagged G 12 D in adipocytes (adipo-G 12 D mice) by crossing the Rosa26-LSL-G12D-IRES-GFP mice (Supplementary Fig. 2a) with the Adipoq-Cre mice.Western blot analysis confirmed its selective expression in adipose tissue (Supplementary Fig. 2b).In the basal state (without CNO administration), body weights and adipose tissue weights were not significantly different between the adipo-G 12 D mice and their control littermates (the Rosa26-LSL-G12D-IRES-GFP mice) (Supplementary Fig. 2c-e).
We next tested the effects of chemogenetic G 12 activation on white-adipose tissue (WAT) browning and glucose homeostasis.Both control and adipo-G 12 D mice were treated with daily intraperitoneal (i.p.) injections of CNO (1 mg/kg) for 5 days prior to tissue collection.RT-PCR analysis showed that the expressions of thermogenic and adipogenic genes in inguinal WAT (iWAT) (Fig. 1c, d) as well as thermogenic genes in brown-adipose tissue (BAT) (Fig. 1e) were not significantly different between the two genotypes.Consistent with these observations, hematoxylin-eosin (H&E) staining showed no obvious morphological changes in both iWAT and BAT (Fig. 1f, Supplementary Fig. 3a).Furthermore, glucose tolerance was unchanged between the two genotypes in both regular chow-and high-fat diet (HFD)-fed conditions (Supplementary Fig. 4a, b).Therefore, activation of G 12 signaling alone does not affect WAT browning, BAT activation, or wholebody glucose homeostasis.
We then investigated the synergistic effects of G 12 activation with G s activation through β3AR stimulation.Chronic administration of the β3AR-selective agonist CL316,243 is a widely used method to induce WAT browning and BAT activation in mice.Both groups of the mice were administered with CL316,243 together with CNO (both 1 mg/kg i.p.) daily for 5 days and evaluated for WAT browning.qRT-PCR analysis revealed a significant upregulation of key thermogenic genes (Ucp1, Cidea, and Elovl3), but not adipogenic genes, in iWAT of the adipo-G 12 D mice (Fig. 1g, h).In the absence of CNO, the induction of the thermogenic genes was comparable between the genotypes (Supplementary Fig. 3f).Western blotting validated increased UCP1 protein levels in the adipo-G 12 D mice (Fig. 1i, j).H&E staining demonstrated the increased numbers of multilocular cells, typical morphology of beige adipocytes in the adipo-G 12 D mice (Fig. 1k, Supplementary Fig. 3d, e).In contrast to the effects observed in iWAT, BAT showed minimal synergistic effects of CNO and CL316,243 on thermogenic gene expression and histology (Fig. 1l, Supplementary Fig. 3b, c).To further analyze the pathways affected by G 12 D activation, we performed RNA-seq transcriptome analysis using iWAT RNA samples.Gene set enrichment analysis (GSEA) revealed that genes upregulated in the adipo-G 12 D mice were involved in oxidative phosphorylation, adipogenesis, and fatty acid metabolism pathways (Fig. 1m, Supplementary Fig. 5), which play key roles in the development of beige adipocytes.The emergence of the adipogenesis characteristics in the analysis is likely attributable to upregulation of genes involved in oxidative phosphorylation and fatty acid metabolism, because these genes also fall under the "adipogenesis" category in the GSEA.Downregulated gene sets were associated with the inflammatory response, such as interferon-α response and TNF-α signaling via NF-κB (Fig. 1m, Supplementary Fig. 5), which is involved in adipocyte dysfunction, including insulin resistance.
To explore the physiological significance of G 12 D-enhanced WAT browning, we evaluated whole-body energy expenditure and adaptive thermogenesis.After simultaneous daily injections of CNO and CL316,243 (both 1 mg/kg i.p.) for 5 days at room temperature, the mice were placed in a metabolic chamber and oxygen consumption was monitored before and after the additional CNO and CL316,243 administration.An acute increase in oxygen consumption was observed in both groups of the mice following the drug administration, a phenomenon attributable to β3AR stimulation (Fig. 1n).In the adipo-G 12 D mice, the elevated oxygen consumption persisted over time, contrasting with the gradual decrease observed in the control mice (Fig. 1n, o).Under this condition, the blood glucose level was higher in the adipo-G 12 D mice while the free-fatty acid level was lower and the glycerol level was unchanged (Supplementary Fig. 6a-d), suggesting that β3AR-induced lipolysis remains unchanged, but fatty-acid uptake is enhanced in the adipo-G 12 D mice.To examine adaptive thermogenesis, after the 5-day drug administration, the mice were placed in a 4 °C cold chamber and and their rectal body temperature was measured.While the control mice showed a rapid decrease in body temperature (peak at 3 h), the decrease rate in the adipo-G 12 D mice was slower (peak at 6 h) (Fig. 1p).Together, these results demonstrate functional significance of G 12 D-induced beige adipocytes.
To investigate whether G 12 D-induced enhancement of WAT browning is controlled in a cell-autonomous manner and to understand the downstream mechanism, we performed a primary culture experiment.Stromal vascular fraction (SVF) was isolated from the adipo-G 12 D mice and differentiated into beige adipocytes in vitro.After differentiation, beige adipocytes were stimulated with CNO in the presence or absence of a series of signaling inhibitors (a Rho kinase (ROCK) inhibitor Y-27632, a G q inhibitor YM-254890, or a myosin II inhibitor blebbistatin).Stimulation by CNO enhanced Ucp1 expression (Fig. 1q), indicating that G 12 D-mediated Ucp1 expression was at least partially cell-autonomous and that constitutive G s signaling was induced in the culture condition.Furthermore, the enhancement of Ucp1 expression was completely inhibited by pretreatment with Y-27632 and blebbistatin, but not by YM-254890 (Fig. 1q).This result demonstrates that G 12 D-mediated Ucp1 expression is dependent on ROCK and myosin II, canonical downstream effectors of G 12 , and is not mediated by potential coupling of G 12 D to G q .
Since activation of G 12 signaling by the DREADD system promoted WAT browning, we searched for G 12 -coupled GPCRs that are endogenously expressed in adipocytes.Using previously published RNA-seq data of isolated mouse iWAT adipocytes, we identified 16 types of G 12 -coupled GPCRs that are expressed in adipocytes (Supplementary Fig. 7).
In conclusion, we used our newly generated the adipo-G 12 D mice to elucidate the effect of chemogenetic activation of G 12 signaling in adipocytes.The G 12 signaling was found to synergistically enhance the beige adipogenesis triggered by the G s -coupled β3AR, thus potentiating the thermogenic effect in vivo.Although we hypothesize that the underlying mechanism is attributed to the synergistic effect downstream of G s and G 12 signaling, it is also conceivable that G 12 signaling boosts the availability of cell-surface β3AR.Moreover, a study using the adpo-G 12 D mice in the G 12 (Gna12)-deficient background will serve as an important validation, which we plan to investigate in the future.Nevertheless, our finding highlights a previously unrecognized role for G 12 signaling as a regulatory pathway of beige adipocyte induction.As G 12 signaling remains uncharacterized in many other tissues, the use of the Cre-driven G 12 D mice will expedite understanding of G 12 signaling in physiology and pharmacology as well as drug development for G 12 -coupled GPCRs.
Fig. 1 Synergistic induction of beige adipogenesis through the G 12 -coupled designer GPCR and the G s -coupled β3AR.a Functional screening of M3D-GPR183/ICL3-derived constructs for G 12 selectivity.G 12 and G o activation was measured by the NanoBiT-G-protein dissociation assay with 10 µM CNO.Note that G-protein activation causes decrease in luminescence counts.Bars and error bars represent the mean and SEM, respectively, of 5-6 independent experiments (dots) with each performed in duplicate.The F 1.57 V mutant of the M3D-GPR183/ICL3 construct (oblique bars) is referred to as G 12 -DREADD or G 12 D. b Concentration-response curves for G-protein activation by the F 1.57 V M3D-GPR183/ICL3 construct.The G-protein-coupling profile was examined by the NanoBiT-G-protein dissociation assay using representative members of the four G-protein subfamilies.Symbols and error bars represent the mean and SEM, respectively, of 3-9 independent experiments with each performed in duplicate.c, d Expression of thermogenic (c) and adipogenic (d) genes in iWAT following CNO single-drug administration (1 mg/kg, i.p., daily) for 5 days (n = 5 per group).e Expression of thermogenic genes in BAT following CNO administration (1 mg/kg, i.p., daily) for 5 days (n = 5 per group).f Representative H&E staining of iWAT following CNO single-drug administration (1 mg/kg, i.p., daily) for 5 days (scale bar: 50 µm).g, h Expression of thermogenic (g) and adipogenic (h) genes in iWAT following CL316,243 and CNO dual-drug administration (1 mg/kg each, i.p., daily) for 5 days (n = 9 for (g) and 6 for (h) per group).i, j Ucp1 protein levels detected by immunoblotting in iWAT of the control and the adipo-G 12 D mice treated dually with CL316,243 and CNO (1 mg/kg each, i.p., daily) for 5 days (n = 5 per group).k Representative H&E staining of iWAT following CL316,243 and CNO dual-drug administration (scale bar: 50 µm).l Expression of thermogenic genes in BAT following 1 mg/kg CL316,243 and 1 mg/kg CNO dual-drug administration for 5 days (n = 5 or 6 per group).m Significantly enriched gene sets (FDR < 0.05) in GSEA.Red: positive NES, blue: negative NES. n, o Whole-body energy expenditure.The mice that were pretreated dually with CL316,243 and CNO (1 mg/kg each, i.p., daily) for 5 days were placed in the metabolic chamber and oxygen consumption rate (VO 2 ) was monitored before and after the dual-drug administration (CL316,243 and CNO, 1 mg/kg each, i.p.; n = 9).Average VO 2 during post 2-h drug administration (o).p Rectal body temperature of the mice treated dually with CL316,243 and CNO (1 mg/kg each, i.p., daily) for 5 days upon acute exposure to 4 °C for the indicated time (n = 12 per group.q Ucp1 expression in primary SVF cells stimulated with 10 µM CNO, with or without pretreatment of 10 µM YM-254890, 10 µM Y-27632, or 50 µM blebbistatin (n = 3 or 4 per group).In all figure panels, bars or symbols, and error bars represent mean and SEM, respectively.Statistical significance was determined by one-way ANOVA followed by the Dunnett's post-hoc test (a), the two-tailed Student's t-test (c-e, g, h, j, l), the two-way ANOVA followed by the Sidak's post-hoc test (p) or the one-way ANOVA followed by the Sidak's post-hoc test (q).*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001