Fig. 6 | Signal Transduction and Targeted Therapy

Fig. 6

From: Eosinophil-derived chemokine (hCCL15/23, mCCL6) interacts with CCR1 to promote eosinophilic airway inflammation

Fig. 6

Inhibition of CCR1 alleviates OVA-induced lung eosinophilic inflammation. a Schematic of BMDE processing and culture timeline representing the treatment of BX471. b Number of eosinophils characterized as SiglecF+F4/80+ cells in WT BMDEs treated with BX471 as indicate in a (n = 4 mice per independent cultures). c Schematic timeline representing the treatment of BX471 in allergic asthma models. d Differential cell counts of cytospin preps represented as the number of BALF cells. e Representative images of lung tissues immunostained with EPX antibody after therapeutic treatment with BX471. Scale bar, 100 μm. f The quantitative percentages of EPX+ cells compared with total nucleated cells analyzed from e (n = 4–5 mice per group, 4 images per mouse). g, h Relative mRNA levels of Il-13 and Il-25 (g) and protein levels of IL-4 and IL-33 (h) in lung tissues determined by quantitative RT-PCR and ELISA. Data are mean ± SEM for 4–5 mice in each group from two independent experiments in d, fh. n.s., not significant; *P < 0.05; **P < 0.01 ***P < 0.001; ****P < 0.0001 by two-way ANOVA in b, one-way ANOVA with Tukey’s post hoc test in d, fh

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