Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Eosinophil-derived chemokine (hCCL15/23, mCCL6) interacts with CCR1 to promote eosinophilic airway inflammation

Fig. 5

mCCL6 directly activates CCR1 and downstream signaling. a Schematic illustration of GloSensor assay in CCR1. Forskolin artificially elevated cAMP levels by activating adenylyl cyclase (AC) whereas CCR1 agonist inhibits AC activity. b Dose-response curves of the intracellular cAMP level measured by GloSensor assay. GloSensor-HEK293T cells transfected with vehicle or mCCR1 were treated with forskolin (1 μM) and indicated concentrations of mCCL6. The reduction of cAMP was recorded after 30 min. Data represent mean ± SEM of three technical replicates (error bars smaller than symbols are not shown). Median effective concentration (EC50) was calculated by nonlinear regression (three parameters). c, d Representative blots of p-ERK1/2, ERK1/2, p-p38, p38 and GAPDH (loading control) assessed by Western blot of protein extracts from mCCR1-293T (c) or vehicle-293T (d) cells cultured with 200 ng/ml mCCL6

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