Fig. 3 | Signal Transduction and Targeted Therapy

Fig. 3

From: Eosinophil-derived chemokine (hCCL15/23, mCCL6) interacts with CCR1 to promote eosinophilic airway inflammation

Fig. 3

CCL6 deficiency alleviates OVA-induced eosinophilic airway inflammation. a Schematic map of established Ccl6−/− mice by using the CRISPR/Cas9 system. b Representative blots of mCCL6 and β-actin (loading control) assessed by Western blot of protein extracts of eosinophils sorted from WT and Ccl6−/− mice. c Differential counts on Wright-Giemsa stained BALF cells in WT and Ccl6−/− mice. OVA-induced asthma model was presented in Fig. 1a. Combined data shown as mean ± SEM are presented for 9–10 mice per group from two independent experiments. Representative photomicrographs of lung sections with H&E staining (d), EPX staining (f), and those with PAS staining (h) at 24 h after the last OVA challenge. Scale bar, 100 μm. Histological inflammatory scores (e) and PAS scores (i) were analyzed from d and h. g The quantitative percentages of EPX+ cells in total nucleated cells analyzed from f (n = 4–5 mice per group, 4 images per mouse). j Relative mRNA levels of Il-13 and Il-25 in lung tissues were determined by quantitative RT-PCR at 24 h after the last NS or OVA challenge. k The concentration of IL-4 and IL-33 in lung tissue determined by ELISA. Data are mean ± SEM for 4–5 mice per group, 5–7 images per mouse. *P < 0.05; **P < 0.01; ***P < 0.001; ****, P < 0.0001 by one-way ANOVA with Sidak’s multiple comparisons test

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