Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Dietary fats suppress the peritoneal seeding of colorectal cancer cells through the TLR4/Cxcl10 axis in adipose tissue macrophages

Fig. 2

HFD potentiates T cell recruitment and activation in tumor-seeded eFats. a HFD consumption promotes the peritoneal metastasis of MC-38 cells in nude mice. Six-week-old male nude mice were intraperitoneally injected with MC-38 cells (1.0 × 106 cells in 100 µl PBS) and immediately fed a CD or HFD for 14 days. Peritoneal tumor nodes were collected and weighed (n = 5). bd HFD consumption enhances T cell infiltration into the eFats. Six-week-old male mice were intraperitoneally injected with MC-38 cells (1.0 × 106 cells in 100 µl PBS) and immediately fed a CD or HFD. Then the stromal cells of the eFats were isolated dynamically. Total T cells (CD45+CD3+CD11bCD45R) (b), CD4+ T cells (CD45+CD3+CD11bCD45R-CD4+CD8) (c), and CD8+ T cells (CD45+CD3+CD11b-CD45R-CD4-CD8+) (d) were assessed by flow cytometry. Each tested sample was pooled from 4 individual samples (n = 3). e HFD consumption increases the frequencies of adipose tissue T cells in the s.c., in situ and Apcmin+/− tumor-seeded models described in “Methods.” Each tested sample was pooled from 2 individual samples (n = 3). f Frequencies of IFNγ+ cells among CD8+ T cells in the eFats of tumor-seeded mice treated with a CD or HFD for 5 days (n = 3). gi Frequencies of IFNγ+ (g), IL17+ (h), and CD25+ (i) cells among CD4+ T cells in the eFats of tumor-seeded mice treated with a CD or HFD for 5 days (n = 3). j Six-week-old male C57BL/6 mice were intraperitoneally injected with MC-38 cells (1.0 × 106 cells in 100 µl PBS) and immediately fed a CD or HFD for 14 days. Each mouse was intraperitoneally treated with antibodies (IgG, anti-CD4, or anti-CD8) 3 times (days 2, 4, and 6) at a dosage of 100 µg day−1. Peritoneal tumor nodes were collected and weighed. (n = 3). All the data are shown as the mean ± s.e.m. The data in bd were analyzed with two-way ANOVA. The data in other histograms were analyzed with Student’s t test (*P < 0.05, **P < 0.01, and ***P < 0.005)

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