Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: Dietary fats suppress the peritoneal seeding of colorectal cancer cells through the TLR4/Cxcl10 axis in adipose tissue macrophages

Fig. 1

High-fat diet suppresses the metastatic seeding of CRC cells. a Metastatic seeding of CRC cells in the epididymal fat (eFat). Six-week-old male mice were intraperitoneally injected with GFP-tagged MC-38 cells (1.0 × 106 cells in 100 µl PBS per mouse) or PBS, which was used as a control. The eFats were collected dynamically and stained with anti-GFP (green) or anti-perilipin (red) antibodies for immunofluorescence analysis. Nuclei were visualized by Hoechst staining (blue). Representative images are shown. Arrows indicate the cancer cells. Scale bars, 100 μm. b High-fat diet (HFD) feeding inhibits tumor seeding in the eFats. Six-week-old male mice were intraperitoneally inoculated with MC-38 cells (1.0 × 106 cells in 100 µl PBS per mouse) and immediately fed a chow diet (CD) or HFD for 14 or 21 days. Then the tumor nodes in the eFats were evaluated. The blue and red arrows indicate the epididymal fat pads and tumor nodes, respectively. c Tumor nodes from the mice described above in b. Representative results for one mouse are shown. d Weights of the tumor nodes described in c. Data are shown as the mean ± s.e.m. (n = 5, ***P < 0.005; Student’s t test). e Dynamic measurement of the weights of the tumor-bearing mice described in b. Data are shown as the mean ± s.e.m. (n = 10, ***P < 0.005; two-way ANOVA). f Dynamic measurement of the food intake of the tumor-bearing mice described in b. Each plot represents the average food intake by one mouse in 1 day (n = 10; two-way ANOVA). g HFD consumption improves the survival of tumor-seeded mice. Tumor-seeded models were established as described in b. This experiment was repeated three times (n = 15, *P < 0.05; Gehan–Breslow–Wilcoxon test). h HFD inhibits the peritoneal seeding of CT-26 cells. Six-week-old male mice were intraperitoneally implanted with CT-26 cells (1.0 × 106 cells in 100 µl PBS) and immediately fed a CD or HFD for 14 days. Then peritoneal tumor nodes were identified. i HFD consumption improves the survival of the tumor-seeded mice described in h. (n = 15, ***P < 0.005; Gehan–Breslow–Wilcoxon test)

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