Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: m6A RNA methylation-mediated HNF3γ reduction renders hepatocellular carcinoma dedifferentiation and sorafenib resistance

Fig. 4

HNF3γ promotes the differentiation of HCC cells. a The expression of hepatocyte-specific genes and AFP in HCCLM3 or Huh7 cells infected with Ad-HNF3γ or Ad-Con was determined by real-time PCR. b Urea concentration in the supernatant of HCCLM3 or Huh7 cells was measured using a QuantiChromTM Urea Assay Kit (upper). The ALB secretion ability of HCC cells was measured as indicated using a Human Albumin ELISA Quantitation kit (lower). Results were expressed as means ± SEM. c The glycogen storage ability of HCCLM3 or Huh7 cells was evaluated by Periodic-Acid Schiff (PAS) staining. Scale bars, 100 mm. d The lipid accumulation ability of HCCLM3 or Huh7 cells was examined by oil red O staining (Sigma-Aldrich). Scale bars, 400 mm. e Hepatocyte differentiation-associated genes differentially expressed in HNF3γ-overexpressing HCCLM3 cells were obtained by RNA-seq and validated by real-time PCR. f HNF3γ-centered regulatory network that includes 12 liver differentiation or metabolism-associated transcription factors and functional molecules. The thickness and brightness of lines indicate the correlation degree between distinct molecules

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