Impact of two ketogenic diet types in refractory childhood epilepsy

Background Ketogenic diet (KD) refers to any diet in which food composition induces a ketogenic state of human metabolism. Objective To assess short- and long-term efficacy, safety, and tolerability of KD [classic KD and modified Atkins diet (MAD)] in childhood drug-resistant epilepsy (DRE) and to investigate the effect of KD on electroencephalographic (EEG) features of children with DRE. Methods Forty patients diagnosed with DRE according to International League Against Epilepsy were included and randomly assigned into classic KD or MAD groups. KD was initiated after clinical, lipid profile and EEG documentation, and regular follow-up was done for 24 months. Results Out of 40 patients with DRE, 30 completed this study. Both classic KD and MAD were effective in seizure control as 60% in classic KD group and 53.33% in MAD group became seizure free, and the remaining showed ≥50% seizure reduction. Lipid profile remained within acceptable levels throughout the study period in both groups. Adverse effects were mild and managed medically with an improvement of growth parameters and EEG during the study period. Conclusions KD is an effective and safe non-pharmacologic, non-surgical therapy for the management of DRE with a positive impact on growth and EEG. Impact Both common types of KD (classic KD and MAD) are effective for DRE, but unfortunately, nonadherence and dropout rates are frequent. High serum lipid profile (cardiovascular AE) is often suspected in children following a high-fat diet, but lipid profile remained in the acceptable level up to 24 months. Therefore, KD constitutes a safe treatment. KD had a positive impact on growth, despite inconsistent results of the KD’s effect on growth. In addition to showing strong clinical effectiveness, KD also considerably decreased the frequency of interictal epileptiform discharges and enhanced the EEG background rhythm.


INTRODUCTION
Up to 65 million people worldwide are affected by epilepsy. 1pproximately one-third of epileptic patients still have difficulty being treated, even though two-thirds of them can control their seizures with current anti-seizure medication (ASM).][8] KD (a high-fat, adequate-protein, and low-carbohydrate diet) is an effective, non-invasive, and non-pharmacologic treatment for refractory childhood epilepsy used since 1920s with few to no neurotoxic effects when compared to multiple ASM. 7,9Ketosis (which mimics a starving state), decreased glucose, higher fatty acid levels (which improve bioenergetic reserves), antiepileptogenic properties and neuroprotective properties are only a few of the various, as-yet-unknown processes through which KD operates. 10][13] Despite the KD's effectiveness, most patients discontinue the diet because of its unpalatable and restrictive features.][16][17][18] MAD provides a more palatable and less restrictive dietary treatment option. 14The most notable aspect of the MAD is that it begins in an outpatient setting without fasting.The effectiveness of MAD in comparison to classic KD is still debatable.Some studies focused on the major AEs of classic KD in refractory epilepsy, while others showed that MAD is equally effective as classic KD. 15,19,20 However, since KD is not a physiological diet, it is important to identify and carefully monitor any AEs. 21AEs can happen at the start of the diet as well as months after KD initiation.Dehydration, altered electrolytes, hypoglycemia, tiredness, abdominal pain, nausea/vomiting, diarrhea, and constipation are short-term AE, while hypoproteinemia, hypocalcemia, hypercalciuria, urolithiasis, alterations in lipid profiles and increase in transaminases or cardiomyopathy are possible long-term AE. 22,23There are few studies available regarding potential long-term negative effects in children using KD for longer than 2 years; the most mentioned symptoms are an increased risk of bone fractures, kidney stones and growth delay. 24lectroencephalographic (EEG) features are improved by KD in addition to significantly reducing clinical seizures in epileptic patients.Despite this, there have not been any reported prospective studies of the predictive power of baseline EEG or early changes in EEG for KD therapy response.In addition, few research have compared the electrophysiologic properties of KD responders and non-responders. 25,26o, this study aimed to assess short-and long-term efficacy, safety, and tolerability of KD (classic KD and MAD) in childhood DRE and to investigate the effect of KD on EEG features of children with DRE.

Study design
This prospective randomized study was conducted to evaluate the efficacy, safety, and tolerability of the KD and its effect on EEG features among children with DRE.
Our primary outcome was to assess the clinical effectiveness of KD (classic KD and MAD) regarding onset of seizure control, seizure frequency and seizure severity, and to assess long-term safety of KD regarding AE and the effect of KD on growth and lipid profile (cardiovascular risk).The secondary outcome was the evaluation of the effect of KD on EEG features prior to and 3 and 6 months after the KD treatment with the possibility of withdrawal of ASM.

Study population
We initially enrolled forty patients with DRE attending Pediatric KD outpatient clinic at Menoufia University Hospital from January 2020 to April 2022, after obtaining the approval of the Institutional Review Boards of the Menoufia Faculty of Medicine (ID number 191019 PEDI 29) and an informed (written) consent was obtained from each parent or caregiver.
Our inclusion criteria were patients who had received two or more types of regular antiepileptic drugs, but frequent seizures continued.Patients with chronic diseases, congenital metabolic disorders, liver diseases, and systemic diseases were excluded from the study.
Patients were randomly assigned to two groups, Group 1 (classic KD group): 20 patients received classic KD in the form of formula (Ketocal milk from Danone, Nutricia) and food with the ratio of 3-4 g of fat for every 1 g of carbohydrate and protein, and Group 2 (MAD group): 20 patients kept on MAD consisting of a nearly balanced diet (60% fat, 30% protein, and 10% carbohydrates by weight) providing 100 kcal/kg/day, without restrictions on calories, fluids, protein or need for an inpatient fast and admission.
Before starting KD, we collected demographic and clinical data of studied patients including age, sex, age of start of seizures, anthropometric measurements (weight, length/height, weight for length/height, and BMI) according to Egyptian Z score growth references for Egyptian children, 27,28 etiology, type of seizures, duration of uncontrolled seizures, seizure frequency, and seizure severity [scored according to Chalfont Seizure Severity Scale (CSSS)]. 29Number of antiepileptic drugs used, and baseline EEG were also documented.

Method of randomization
The allocation sequence was generated using permuted block randomization technique and the block size was variable. 30Allocation sequence/code was concealed from the person allocating the participants to the intervention arms using sealed opaque envelopes. 31Double-blinded approach was adopted. 32In the present study, consecutive sampling technique was adopted. 33

Initiation of KD
For the first month, carbohydrates were restricted to 10 g/day but were permitted to increase by 5 g/day at intervals of at least 1 month if the child was having difficulty with the restriction of carbohydrates to a maximum of 10% carbohydrates per day by weight.A qualified dietician also educated the parents or caregivers about diet preparation at home with close monitoring of blood glucose and urinary ketones.Multivitamins, calcium, and vitamin D were given as supplements and the antiepileptic drugs and doses used were not changed from those administered previously throughout the study period.Patients were requested to attend their regular monthly outpatient visits for 6 months, then every 3 months for 24 months and the form and frequency of seizures and adverse reactions were observed and recorded.

KD tolerability
After 1 month of KD initiation, two patients of classic KD group and one patient of MAD group were excluded from the study due to noncompliance of their caregivers.At 3-month follow-up visit, six patients were excluded from the study as they did not tolerate the MAD and the ketogenic liquid formula.Also, one patient of MAD group died secondary to infection in COVID-19 epidemic, so only 30 patients completed the study for 2 years, 15 in each group.

Short-term outcome
The clinical efficacy of KD was evaluated regarding the onset of improvement of seizures and seizures frequency and severity (according to CSSS) prior to and 3 and 6 months after the KD treatment were analyzed.Effectiveness was evaluated as complete seizure free or seizure reduction percentage change.Percentage change was calculated as follows:

KD and EEG
The NIHON KOHDEN EEG-1200K was used to record the EEG in a quiet environment.The 21-channel-EEGs were recorded under typical circumstances (rest, hyperventilation, and photostimulation).Throughout the recording period, children were being studied while resting off with their eyes closed.The International 10-20 system was used to record EEG data from 19 scalp electrodes for 20 min using an average reference (MIZARsirius 33 Channels, EBNeuro). 35t FP1, F3, C3, P3, O1, F7, T3, T5, FP2, F4, C4, P4, O2, F8, T4, T6, Fz, Cz, and Pz, electrodes were placed.The EEG was digitized at 256 Hz with a time constant of 0.1 sec, a high-frequency filter of 70 Hz and a notch filter in each channel.Based on visual assessment, consecutive, 2-s-long epochs free of artifacts were chosen offline.The provided EEG software was used to perform a rapid Fourier transform on these 20 epochs.Mean-power spectra were gathered for every channel and frequency range in each subject.Six bands were utilized to divide the frequency ranges: Delta (0-4 Hz), Theta (4-8 Hz), Alpha (8-12 Hz), Beta-1 (12-18 Hz), Beta-2 (18-24 Hz), and Gamma (24-64) were the six bands used to separate the frequency ranges. 35y comparing EEG before and 1, 3 and 6 months after the KD therapy regarding the background rhythm in the occipital region under the awake and silent states and variations of interictal spike-wave index (SI), the effect of KD on EEG features was assessed.SI represented the mean of the spike discharge times during a second.In the calculation procedure, 100 s of the awake and quiet EEG findings (free of artifact fragments) were chosen, and SI = n/100 was used to compute the number of spikes (n) present in the recording.

Long-term outcome
Anthropometric measurements, lipid profile, and the development of AE were monitored at 3-month intervals up to 24 months.In addition, we assessed the possibility of antiepileptic drug withdrawal in seizure free patients after the first 6 months.

Statistical methodology
Data were collected and entered into the computer using Statistical Package for Social Science program for statistical analysis (ver 25).Data were described using median and interquartile range.Comparisons were carried out between two studied independent not-normally distributed subgroups using the Mann-Whitney U test.Comparisons were carried out among related samples by Friedman's test.Odds ratio (OR) was used to quantify the strength of the association between two events.McNemar's test was used on paired nominal data with matched pairs of subjects to determine whether the row and column marginal frequencies were equal.Statistical significance was tested at P value < 0.05.
Based on El-Rashidy et al.'s 18 results, adopting a power of 80% to detect a non-inferiority margin (d) of 15% in success rate [complete disappearance of seizures (primary outcome)] and level of significance 5% (α = 0.05), the minimum required sample size was found to be 15 patients per group (number of groups = 2) and the total sample size = 30 patients. 36,37

RESULTS
Out of 40 patients enrolled initially in our study, 30 patients (13 males and 17 females) with median age 3 years (36 months) in classic KD group versus 6 years (72 months) in MAD group tolerated this study for 24 months, 11 patients (36.67%) were underweight, 3 patients (10%) were overweight, and 7 patients (23.33%) were wasted with non-significant difference between the two groups.Patient characteristics are listed in Table 1.

Clinical presentation
According to ILAE (2017), 38,39 epilepsy of unknown etiology was the commonest etiology among the participants [12 patients (40%)] and Myoclonic seizure was the most prevalent type [10 cases (33.33%)] followed by infantile spasm [6 cases (20%)].The median duration of uncontrolled seizures before KD was 7 months in classic KD group versus 8 months in MAD group with a median seizure severity scale (according to CSSS) and seizure frequency per day of 165 and 10, respectively.Sixteen patients (53.33%) were on three AEDs, ten (33.33%) patients were on four AEDs and four patients (13.33%) were on two AEDs.The clinical characteristics of the cases before KD are illustrated in Table 2.

Efficacy of KD
The onset of seizure improvement was 14 days in classic KD group versus 10 days in MAD group with a non-significant difference between the two groups.Regarding the CSSS, there was a statistically significant decrease in seizure severity by −100% percent change after 3 and 6 months of KD compared to baseline (P < 0.0001) without a significant difference between the two groups (Table 3).Analysis of CSSS after 6 months of KD initiation demonstrates improvement of duration of seizures in all patients and improvement in time to return to normal from the onset in 96.67% of patients.Figure 1 illustrates the improvement in CSSS after 3 and 6 months of KD treatment.
Seizure frequency after 3 and 6 months of KD showed a statistically significant decrease in comparison with baseline (P < 0.0001) with a non-significant difference between both groups.Six months after initiation of KD, 60% of patients in classic KD group and 46.67% of patients in MAD group became seizure free and the other 40% and 53.33% had ≥50% seizure reduction with a median percent decrease of −83.33% and −75% after 3 months in classic KD and MAD, respectively, and −100% after 6 months in both groups (Table 4).The best seizures control was observed in genetic epilepsy, Sturge-Weber syndrome, tuberous sclerosis, Miller-Dieker syndrome and white matter disease causing leukodystrophy, each of them (11 cases) became seizure free 6 months after KD, followed by cases of post-traumatic epilepsy and post-anoxic epilepsy who responded well to the KD treatment with 2 out of 3 and 3 out of 4, respectively, were seizure free and seizures in the remaining 1/3 and 1/4 of them were reduced by >80-90%.On the other hand, the efficacy of KD for epilepsy of unknown etiology (12 cases) was poor as no patients became seizure free and seizure reduction ranged from >50 to <80%.
In addition to seizure control, we observed that 46.67% of patients in classic KD group and 66.67% of patients in MAD group showed attention improvement 6 months after initiation of KD.

KD and EEG
KD had an impact on the EEG after the first month, and after 3 months, improvements in the background rhythm slowing were observed by plus ≥2 Hz in 8 cases and by plus 1-2 Hz in 15 cases, while after 6 months was observed by plus ≥2 Hz in 9 cases and by plus 1-2 Hz in 15 cases compared to baseline with significant correlation with seizure control after 6 months (P value = 0.031 and 0.021), respectively (Table 5).In addition, no change in background rhythm (<1 Hz) which was found in 20 out of 30 cases after 1 month, considerably improved to continue in just 7 cases after 3 months, and in only 6 cases after 6 months, with a significant correlation with seizure control (P value < 0.001).Figure 2 illustrates changes in background rhythm slowing after 1, 3 and 6 months of KD.
There was a statistically significant difference regarding seizures control between cases with baseline EEG with no epileptiform discharge and cases with baseline EEG with epileptiform discharge after 3 and 6 months of KD as all 8 cases with baseline EEG with no epileptiform discharge became seizures free 3 months after KD compared to cases with baseline EEG with epileptiform discharge only 4/22 and 8/22 became seizures free at 3 and 6 months (P value = 0.006 and 0.026) respectively.Moreover, the interictal SI among cases with baseline EEG with epileptiform discharge (22  cases, 11 in each group) showed a significant reduction of >50% after 1 month in 8/22 then this increased to 14 cases after 3 months and 16 cases after 6 months.Also, SI reduction <30% significantly reduced from 8 cases after 1 month to only 2 cases after 6 months with a significant correlation with seizure control (P = 0.031).The details of EEG changes in response to KD and its correlation with seizure control are illustrated in Table 5.

KD and ketosis
Our results did not find any correlation between the level of urinary ketones and seizure control 3 and 6 months after KD with a P value of 0.197 and OR 0.300.

Adverse effects
Our results revealed that gastrointestinal (GI) complications were frequent in our studied cases and constipation was the most common with the same occurrence in the two groups (33.33%), followed by diarrhea with a lower occurrence in classic KD group (13.33%) than MAD group (20%) then vomiting (20%) in classic KD group and (6.67%) in MAD group.We did not document renal/ genitourinary complications in our study.
Long-term outcome KD and cardiovascular risk.In our study, median level of HDL decrease did not reach a low level till the end of the study for 24 months.Also, median level of LDL, total cholesterol, and TG increase did not reach a high level (remained in the acceptable level) throughout the study period, without a significant difference between classic KD group and MAD group.Figure 3 demonstrates serial measurements of lipid profile from baseline up to 24 months.
KD and antiepileptic drugs.Upon regular follow-up visits every 3 months after the first 6 months, it was possible to withdraw one AEDs in three patients (20%) of classic KD group and five patients (33.33%) of MAD groups, in addition to withdrawal of two AEDs in three patients (20%) in classic KD group and two patients (13.33%) in MAD group leading to less AEDs side effects and better QoL while on adequate seizure control by KD.KD and growth.The current study found a positive impact of KD on growth of studied cases (weight loss and BMI reduction were minimal) except in patients who were significantly overweight at diet initiation.Median weight and BMI of cases up to 24 months are illustrated in Fig. 4.

DISCUSSION
Forty patients with DRE were initially enrolled in this prospective study, but only 30 patients successfully completed it.Six patients (15%) were unable to tolerate KD and were removed from the study (85% tolerability), three additional patients were excluded due to parental noncompliance and one case died during the study period.Overall reasons for dropout were mostly intolerance of the diet, AEs (mostly GI tract related), weight loss, parental unhappiness and change of mind. 40eta-analysis studies have confirmed the efficacy of the KD and showed a seizure frequency reduction (SFR) of ≥50% for both the classic KD and MAD. 41Our findings revealed that 60% of patients in classic KD group and 46.67% of patients in MAD group became seizure free and the other 40% and 53.33%, respectively, had ≥50% SFR.Also, the effectiveness of the KD treatment showed an increased tendency over time, with a median decrease in seizure frequency of −83.33% and −100% versus −75% and −100% after 3 and 6 months in the classic KD group and MAD group respectively compared to patients' baseline.
With a median percent change of −100%, we also documented a statistically significant reduction in seizure severity based on the CSSS 3 and 6 months after the KD compared to baseline in both groups.Two RCTs reported a statistically significant decrease in seizure severity, El-Rashidy et al. 18 with a mean reduction in seizure severity of 37.63% (MAD) and 35.89% (KD) after 6 months, and Lambrechts et al. 42 with a mean reduction of 65.2%.
The KD enhanced patients' cognitive and functional status while also reducing seizure severity and frequency.Six months following KD, we noticed improvements in functional status and cognition, with 66.67% of patients in the MAD group and 46.67% of patients in the classic KD group displaying better attention.This is essential for DRE patients since uncontrolled seizures and the use of numerous anticonvulsants may negatively impact cognition, behavior, drowsiness, memory, and attention issues. 43,44These cognitive enhancements might be attributable to the KD given as the mean number of AEDs did not change until 6 months after the diet therapy.
6][47][48] In our study, all 3 patients with focal seizures, 4/5 of patients with generalized tonicclonic seizures, 3/6 with infantile spasms, 5/10 with myoclonic seizures and 1/6 with unclassified seizures became seizure free after 6 months after KD initiation with no significant difference between the two groups.However, because of the small sample size, we were unable to draw any conclusions about which type of seizure was linked to better seizure control.
Although the KD has been proven to be a successful treatment for reducing seizures in DRE patients, its wider effects on cerebral neurophysiology are less clear. 49KD not only demonstrated good clinical efficacy in this study, but it also significantly reduced the frequency of interictal epileptic discharges and improved the EEG background rhythm.This was evident in 22 patients (73.33%) who had baseline EEG with epileptiform abnormalities (11 in each group) with a reduction in the SI >50% in 8 patients after 1 month, which increased to 14 patients after 3 months and 16 after 6 months.Zhu et al. 50reported ≥50% reduction in seizure frequency and reduction of epileptiform discharges in the awake state in 69.0% of patients after 3 months of KD treatment.
KD side effects are frequently blamed for trial dropouts since they are observed in a high percentage of young patients. 7More than forty different types of AEs were found with cardiovascular, renal/ genitourinary, skeletal systems, and GI (mainly constipation) being the most prevalent. 22,51In our study, the most common AEs were GI related, with constipation reported in 33.33% of patients in each group and we did not report any renal/genitourinary AEs in our study.In general, AEs were transient, well controlled by conservative management and did not necessitate for diet discontinuation.Lipid profile underwent frequent changes throughout the study period.However, HDL median levels did not reach the low level and the median of LDL, total cholesterol and TG measurements remained within the acceptable range till the end of the study for 24 months with a non-significant difference between classic KD and MAD.Kossoff et al., in 2006, 2007, and 2008 over the course of these investigations, observed that there was a comparable increase in total cholesterol and LDL levels, which was within the accepted value. 14,52,53Contrarily, Coppola et al. 54 observed hyperlipidemia as a side effect in their group of patients receiving liquid ketogenic formula for refractory epileptic encephalopathies.][57] Acceptable change of lipid profile with a long-term duration of KD (24 months) can be a good indicator for the safety of high-fat diet on cardiovascular system in children with DRE.
Systematic reviews have discovered conflicting results about the KD's effects on growth, with some indicating a favorable benefit and others indicating a negative impact. 55Poor caloric and protein intake, acidosis or ketosis, the effects of underlying illnesses and therapies, ambulatory status, and related endocrine changes are some of the etiologies of poor growth in children on KDTs. 58,59The current study found that children who were underweight at diet onset [11 (36.67%) underweight and 7 (23.33%)wasted] showed an increase in Z scores of child weight over time.However, those who were overweight at diet onset and remained on the diet showed a decrease in the Z score over the longer term.This positive impact on growth highlights that fears from KD regarding weight loss were not evident.
Due to their excellent response to the diet, it was possible to withdraw AEDs in 13 patients (43.33%) after the first 6 months: one AEDs in 8 patients (3 in classic KD group vs. 5 in the MAD group) and two AEDs in 5 patients (3 in classic KD group vs. 2 in the MAD group).This can be attributed to the patients' functional status and QoL.
The study's points of strength include its prospective nature, which increased the accuracy of our data.We studied two types of KD (classic KD and MAD) commonly used for DRE to allow more options and less diet restrictions.In addition, the effectiveness of the KD regarding seizure control was not measured based solely Bold values refer to the total number of cases who became seizure-free or showed seizure frequency reduction 3 months after KD and followed by details of seizure frequency reduction.on caregiver reports but included the EEG changes so allowing more objective evaluation and avoiding subjective errors.Along with growth tracking and documenting of the KD's effects on growth, we also evaluated the KD's safety for up to 24 months in terms of side effects and cardiovascular risk.

LIMITATION OF THE STUDY
The main limitations of our study were the small sample size and the heterogeneity of the enrolled patients, which influenced the lack of statistical significance.Another point of limitation was the dropped-out cases due to noncompliance as we started the study with 40 patients and only 30 patients completed the study; the most common reasons for discontinuing the diet were intolerability and poor parental compliance when maintaining the diet.

CONCLUSION
With no major side effects reported and a positive influence on EEG and growth, KD (classic KD and MAD) appears to be an

Fig. 1
Fig. 1 Chalfont seizures severity scale 3 and 6 months after ketogenic diet.Improvement by percentage in Chalfont Seizure Severity Scale (item by item) after 3 and 6 months of intervention.

Fig. 2
Fig. 2 Changes in background rhythm slowing after 1, 3 and 6 months of ketogenic diet.

Fig. 3
Fig. 3 Lipid profile of cases throughout the study (24 months).a Simple line graph of median of serum HDL (mg/dL) in the studied groups.b Simple line graph of median of serum LDL (mg/dL) in the studied groups.c Simple line graph of median of serum total cholesterol (mg/dL) in the studied groups.d Simple line graph of median of serum triglycerides (mg/dL) in the studied groups.

Fig. 4
Fig. 4 Weight and body mass index of cases throughout the study (24 months).a Simple line graph of median (95% CI) of BMI SD in the studied groups.b Simple line graph of median (95% CI) of weight SD in the studied groups.

Table 1 .
Demographic data and anthropometry of studied cases.

Table 2 .
Clinical characteristics before KD.
NS non significant, NA non-applicable.

Table 3 .
Seizures severity after KD.Friedman Chi-Square, df degree of freedom.Intragroup: statistically significant when compared with baseline values (using Dunn-Sidak method).
Time to return to normal from onsetImprovement in Chalfont Seizures severity scale (Item by item) after 3 and 6 months of intervention

Table 5 .
EEG changes in response to KD and its correlation with seizure control.