Genetic variant burden and adverse outcomes in pediatric cardiomyopathy

Abstract

Background

Previous genetic research in pediatric cardiomyopathy (CM) has focused on pathogenic variants for diagnostic purposes, with limited data evaluating genotype-outcome correlations. We explored whether greater genetic variant burden (pathogenic or variants of unknown significance, VUS) correlates with worse outcomes.

Methods

Children with dilated CM (DCM) and hypertrophic CM (HCM) who underwent multigene testing between 2010 and 2018 were included. Composite endpoint was freedom from major adverse cardiac event (MACE).

Results

Three hundred and thirty-eight subjects were included [49% DCM, median age 5.7 (interquartile range (IQR) 0.2–13.4) years, 51% HCM, median age 3.0 (IQR 0.1–12.5) years]. Pathogenic variants alone were not associated with MACE in either cohort (DCM p = 0.44; HCM p = 0.46). In DCM, VUS alone [odds ratio (OR) 4.0, 95% confidence interval (CI) 1.9–8.3] and in addition to pathogenic variants (OR 5.2, 95% CI 1.7–15.9) was associated with MACE. The presence of VUS alone or in addition to pathogenic variants were not associated with MACE in HCM (p = 0.22 and p = 0.33, respectively).

Conclusion

Increased genetic variant burden (pathogenic variants and VUS) is associated with worse clinical outcomes in DCM but not HCM. Genomic variants that influence DCM onset may be distinct from those driving disease progression, highlighting the potential value of universal genetic testing to improve risk stratification.

Impact

  • In pediatric CM, inconsistent findings historically have been shown between genotype and phenotype severity when only pathogenic variants have been considered.

  • Increased genetic variant burden (including both pathogenic variants and VUS) is associated with worse clinical outcomes in DCM but not HCM.

  • Genomic variants that influence CM onset may be distinct from those variants that drive disease progression and influence outcomes in phenotype-positive individuals.

  • Incorporation of both pathogenic variants and VUS may improve risk stratification models in pediatric CM.

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Fig. 1
Fig. 2: Distribution of genetic variants across dilated cardiomyopathy (left) and hypertrophic cardiomyopathy (right) cohorts.
Fig. 3
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Acknowledgements

This work was supported, in part, by the Cardiac Center Clinical Research Core at the Children’s Hospital of Philadelphia. No specific support was provided for this project.

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D.S.B. conceptualized and designed the study, drafted the initial manuscript, and reviewed and revised the manuscript. R.C.A.-N. reviewed and classified all genetic variants. M.J.O’C., H.G., A.R., K.Y.L., and J.W.R. designed the data collection instruments, collected data, carried out the initial analyses, and reviewed and revised the manuscript. J.W.G. and R.C.A.-N. conceptualized and designed the study, coordinated and supervised data collection, and critically reviewed the manuscript for important intellectual content. All authors approved the final manuscript as submitted and agree to be accountable for all aspects of the work.

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Correspondence to Danielle S. Burstein.

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Burstein, D.S., Gaynor, J.W., Griffis, H. et al. Genetic variant burden and adverse outcomes in pediatric cardiomyopathy. Pediatr Res (2020). https://doi.org/10.1038/s41390-020-1101-5

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