Fig. 5: HPDE overexpressing miR-93 proliferate, migrate, and invade more than control cells. | Oncogenesis

Fig. 5: HPDE overexpressing miR-93 proliferate, migrate, and invade more than control cells.

From: MiR-93 is related to poor prognosis in pancreatic cancer and promotes tumor progression by targeting microtubule dynamics

Fig. 5

a MiR-93 expression in pancreatic tissue samples and pancreatic cell lines. PDAC tissues (n = 50), healthy pancreatic tissues (n = 25) and PANC-1 (n = 3) and HPDE (n = 3) pancreatic cell lines. MiR-93 expression normalized against RNU6B. b MTS assay to assess the effects of miR-93 overexpression on cell viability (n = 4). c Western Blot of Ki-67 and Cyclin D1 normalized against β-actin on HPDE-hTR and HPDE-miR-93 protein extracts (representative image of n = 3). d Wound healing assay to assess the effects of miR-93 on cellular motility by calculating per cent wound closure over time points (n = 11). Representative images are shown. e Transwell invasion assay of HPDE-miR-93 expressing cells compared with HPDE-hTR control cells (n = 3). Representative images are shown. Error bars = s.d.; **p ≤ 0.01; ***p ≤ 0.001.

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