a Kaplan–Meier curves showing (a) the percentage of mice free of visible retinoblastoma tumors in Rb1/p107 DKO (n = 18) and Rb1/p107/Hells (n = 25) and (b) the time to moribund status after tumor detection of retinoblastoma-bearing animals mice in Rb1/p107 DKO (n = 13) and Rb1/p107/Hells (n = 4). Mantel–Cox test was used for curve comparisons. c Western blot detection of HELLS confirms loss of HELLS protein in Rb1/p107/Hells TKO tumors and HELLS expression in Rb1/p107 DKO tumors. Tubulin used as a loading control. d Principal components analysis (PCA) (3D loading plot) of RNA-seq data from three Rb/p107 DKO and three Rb1/p107/Hells TKO tumors show the absence of distinct transcriptome clusters.