Fig. 2: TBX3 directly represses TBX2 in RH30 cells. | Oncogenesis

Fig. 2: TBX3 directly represses TBX2 in RH30 cells.

From: TBX3 represses TBX2 under the control of the PRC2 complex in skeletal muscle and rhabdomyosarcoma

Fig. 2

a pEF-TBX3 (TBX3) or pEF-empty vector (EV) was stably transfected into RH30 cells and the expression of TBX3 in three independent isolates was assayed by qRT-PCR analysis. Error bars, S.E. and ***p ≤ 0.001 versus EV. b Expression of TBX2 in the cells described in (a) was measured by qRT-PCR analysis. Error bars, S.E. and **p ≤ 0.01, ***p ≤ 0.001 versus EV. c Protein extracts from the stably transfected isolates in (a) were used for western blot analysis with antibodies against the indicated proteins. GAPDH was used as a loading control. d TBX3 binds to the TBX2 promoter. ChIP assays were performed on RH30 EV and RH30 TBX3-1 cells with antibodies against TBX3 or IgG and primers to the TBX2 promoter. Error bars, S.E. and **p ≤ 0.01, ***p ≤ 0.001 versus IgG

Back to article page