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CSDE1 attenuates microRNA-mediated silencing of PMEPA1 in melanoma

Oncogene volume 40, pages 3231–3244 (2021)Cite this article

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Abstract

MicroRNAs and RNA-binding proteins (RBPs) primarily target the 3′ UTR of mRNAs to control their translation and stability. However, their co-regulatory effects on specific mRNAs in physiology and disease are yet to be fully explored. CSDE1 is an RBP that promotes metastasis in melanoma and mechanisms underlying its oncogenic activities need to be completely defined. Here we report that CSDE1 interacts with specific miRNA-induced silencing complexes (miRISC) in melanoma. We find an association of CSDE1 with AGO2, the essential component of miRISC, which is facilitated by target mRNAs and depends on the first cold shock domain of CSDE1. Both CSDE1 and AGO2 bind to 3′ UTR of PMEPA1. CSDE1 counters AGO2 binding, leading to an increase of PMEPA1 expression. We also identify a miRNA, miR-129-5p, that represses PMEPA1 expression in melanoma. Collectively, our results show that PMEPA1 promotes tumorigenic traits and that CSDE1 along with miR-129-5p/AGO2 miRISC act antagonistically to fine-tune PMEPA1 expression toward the progression of melanoma.

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Fig. 1: Target mRNA facilitates the interaction between CSDE1 and AGO2 in melanoma.
Fig. 2: CSDE1 and AGO2 bind common targets on the 3′ UTR.
Fig. 3: CSDE1 promotes the expression of PMEPA1 in melanoma.
Fig. 4: The miRNA pathway controls PMEPA1 expression in melanoma.
Fig. 5: CSDE1 and AGO2 compete to bind PMEPA1 mRNA.
Fig. 6: CSDE1 interaction with AGO2 regulates PMEPA1 expression.
Fig. 7: PMEPA1 promotes oncogenesis in melanoma.

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Acknowledgements

We thank all members of our laboratory for helpful discussions and the Bioinformatics Unit at CRG Barcelona for their services in analyzing the CLiP-Seq data.

Funding

The Canadian Institutes of Health Research (CIHR) supported this work. PKK and LMH are recipients of scholarship from Fonds de Recherche du Québec-Santé (FRQ-S). MJS is a Research Chair from FRQ-S. LG was supported by the CIHR (FDN-143213). FG was supported by grants from the Spanish Ministry of Science and Innovation (MICINN, PGC2018-099697-B-I00), “la Caixa” Foundation (ID:100010434) under the agreement LCF/PR/HR17/52150016, the Catalan Government (2017SGR534) and the Center of Excellence Severo Ochoa.

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Authors and Affiliations

  1. CHU de Québec—Université Laval Research Center (Oncology Division), Québec, QC, Canada

    Pavan Kumar Kakumani, Francois Houle, Louis-Mathieu Harvey & Martin J. Simard

  2. Université Laval Cancer Research Centre, Québec, QC, Canada

    Pavan Kumar Kakumani, Francois Houle, Louis-Mathieu Harvey & Martin J. Simard

  3. Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain

    Tanit Guitart & Fátima Gebauer

  4. Centre de recherche du CHU de Québec-Université Laval (Axe Médecine Régénératrice) and Centre de recherche en organogénèse expérimentale de l’Université Laval/LOEX, Québec, QC, Canada

    Benjamin Goyer & Lucie Germain

  5. Département de chirurgie, Faculté de médecine, Université Laval, Québec, QC, Canada

    Benjamin Goyer & Lucie Germain

  6. Universitat Pompeu Fabra (UPF), Barcelona, Spain

    Fátima Gebauer

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Contributions

PKK designed and performed most of the experiments. TG and FH performed cancer assays, while LMH helped in the preparation of different reagents. TG also generated data for Fig. 3D, E. MJS and FG supervised the work. BG isolated, cultured, and prepared proteins from melanocytes used in Fig. 3E and was supervised by LG. PKK and MJS wrote the manuscript with the help of FG.

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Correspondence to Pavan Kumar Kakumani or Martin J. Simard.

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Kakumani, P.K., Guitart, T., Houle, F. et al. CSDE1 attenuates microRNA-mediated silencing of PMEPA1 in melanoma. Oncogene 40, 3231–3244 (2021). https://doi.org/10.1038/s41388-021-01767-9

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