Fig. 2: MAO-A is a directly regulated GR target and is associated with elevated GR activity. | Oncogene

Fig. 2: MAO-A is a directly regulated GR target and is associated with elevated GR activity.

From: Targeting the glucocorticoid receptor signature gene Mono Amine Oxidase-A enhances the efficacy of chemo- and anti-androgen therapy in advanced prostate cancer

Fig. 2

A MAO-A mRNA expression in PF179TCAF-shGR-1 cells after 24 h single or combination treatments with 100 nM Dex, 100 nM Pred, 1 µg/ml Dox, and 12 µM RU486 for 24 h. Data represent mean + SE from at least three independent experiments (one-way ANOVA and correction for multiple testing using Bonferroni´s comparison test; ***, P < 0.001). B Identification of 2 GR binding sites (R1 and R2) near the MAO-A gene in LREX´, LNCaP-1F5, VCaP, A549, Beas-2B, and HepG2 cells, screening publicly available ChIP-Seq datasets. C Time course of MAO-A and GILZ mRNA expression after incubation with 100 nM Dex. Data represent mean + SE from three independent experiments. D GR-ChIP with PF179TCAF cells after treatment with 100 nM Dex alone, or in combination with 6 µM RU486 for 16 h. Data represent mean + SE from three independent experiments (one-way ANOVA and correction for multiple testing using Bonferroni´s comparison test; *, P < 0.05). E, F MAO-A mRNA and protein expression after 100 nM Dex treatment for 3 d within different standard PCa cell models. mRNA data represent mean + SE from three independent experiments (unpaired t-test; *, P < 0.05; **, P < 0.01). G, H MAO-A is significantly positively correlated with GR activity within the publicly available TCGA-PRAD and SU2C-PRAD datasets.

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