Fig. 8: rIL-17B promoted autophagy activation and ubiquitination of Beclin-1 by inducing IL-17RB expression. | Oncogene

Fig. 8: rIL-17B promoted autophagy activation and ubiquitination of Beclin-1 by inducing IL-17RB expression.

From: IL-17B/IL-17RB signaling cascade contributes to self-renewal and tumorigenesis of cancer stem cells by regulating Beclin-1 ubiquitination

Fig. 8

A The relationship between IL-17B in the serum and the expression of IL-17RB mRNA in GC tissues from the same patient. B qRT-PCR was used to detect IL-17RB mRNA expression in MGC-803 cells treated with rIL-17B or PBS for 48 h (n = 3; ***p < 0.001). C Immunofluorescence was used to assay the IL-17RB protein expression in MGC-803 cells treated with rIL-17B or PBS for 48 h. D After transfection with different concentrations of Flag-IL-17RB and HA-Beclin-1 (2 μg) plasmids, 293T cells were subjected to IP using an HA antibody or control IgG, followed by IB with K63-linked ubiquitin, IL-17RB, Beclin-1, and GAPDH antibodies. E After transfection with different concentrations of Flag-IL-17RB, HA-Beclin-1, and MYC-TRAF6 plasmids, 293T cells were subjected to IP using an HA antibody or control IgG, followed by IB with HA, MYC, and IL-17RB antibodies. F Deletion of the TRAF6-binding domain in IL-17RB (Flag-IL-17RB) abolished recruitment and binding between TRAF6 and Beclin-1.

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