Fig. 6: rIL-17B reinforced K63-mediated ubiquitination of Beclin-1 by promoting TRAF6 binding to Beclin-1. | Oncogene

Fig. 6: rIL-17B reinforced K63-mediated ubiquitination of Beclin-1 by promoting TRAF6 binding to Beclin-1.

From: IL-17B/IL-17RB signaling cascade contributes to self-renewal and tumorigenesis of cancer stem cells by regulating Beclin-1 ubiquitination

Fig. 6

A IP showing the expression of ubiquitin, Beclin-1, or GAPDH in rIL-17B- or PBS-treated MGC-803 cells transfected with the HA-Beclin-1 plasmid and treated with MG132 (20 μM) for 6 h. B IP revealed lysine 63-linked ubiquitin, Beclin-1, or GAPDH in rIL-17B- or PBS-treated MGC-803 cells transfected with the HA-Beclin-1 plasmid and treated with MG132 (20 μM) for 6 h. C MGC-803 cells were transfected with HA-Beclin-1 and TRAF6 interference oligonucleotide sequence (TRAF6 siRNA). The indicated HA pull-down samples were analyzed using Western blotting for the presence of K63-linked ubiquitin. The expression of TRAF6, Beclin-1, and GAPDH was detected in the total protein (input). D Super-resolution microscopy was used to analyze the binding between TRAF6 and Beclin-1 in MGC-803 cells transfected with HA-Beclin-1 and the MYC-TRAF6 plasmid treated with rIL-17B or PBS for 48 h. E IP showing HA or MYC in rIL-17B- or PBS-treated MGC-803 cells transfected with HA-Beclin-1 and the MYC-TRAF6 plasmid.

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