Fig. 5: rIL-17B promoted self-renewal of GC cells by activating autophagy. | Oncogene

Fig. 5: rIL-17B promoted self-renewal of GC cells by activating autophagy.

From: IL-17B/IL-17RB signaling cascade contributes to self-renewal and tumorigenesis of cancer stem cells by regulating Beclin-1 ubiquitination

Fig. 5

A Confocal microscopy was used to detect LC3 protein expression in MGC-803 cells treated with rIL-17B or PBS. B Western blotting was used to assay the expression of LC3, p62, and GAPDH in MGC-803 cells treated with rIL-17B or PBS for 48 h. C TEM was used to analyze autophagosome formation in MGC-803 cells treated with rIL-17B or PBS for 48 h (blue arrow for autophagolysosome structure). D The spheroids in shControl or shATG7 lentiviral-infected MGC-803 cells treated with rIL-17B or PBS for 48 h were detected. E Spheroid numbers with diameter > 50 μm under ×40 microscope magnification were statistically analyzed (n = 6; ***p < 0.001; ns: not statistically significant). F qRT-PCR was used to detect OCT4, NANOG, SOX2, and LGR5 in shControl or shATG7 lentiviral-infected MGC-803 cells treated with rIL-17B or PBS for 48 h (n = 6; ***p < 0.001; ns not statistically significant).

Back to article page