a WB analysis of SFPQ expression in normal human epithelial ovarian cells (HuNoEC) and in the indicated EOC cell lines. qRT-PCR (b) and WB analyses (c) reporting the normalized SFPQ expression in samples derived from untreated and PT-exposed (after neo-adjuvant chemotherapy or recurrent) EOC patients. A representative WB analysis of SFPQ expression is shown in (c, bottom panel). d qRT-PCR analysis reporting the normalized SFPQ mRNA expression in samples from PT-sensitive and PT-resistant EOC patients. e WB evaluating the expression of SFPQ and p54nrb and of DNA damage (γ-H2AX) and apoptotic markers (PARP-1 and cleaved caspase-3) in parental and isogenic PT-resistant (MIres) MDAH cells treated or not with CDDP for 16 h (t0) and then released for 24 h (t24). In the figure statistical significance was determined by a two-tailed, unpaired Student’s t test. (*p < 0.05; **p < 0.01; ***p < 0.001). Vinculin or GAPDH expression was used as loading control.