Fig. 1: SFPQ modulates PT sensitivity in epithelial ovarian cancer cells. | Oncogene

Fig. 1: SFPQ modulates PT sensitivity in epithelial ovarian cancer cells.

From: Splicing factor proline- and glutamine-rich (SFPQ) protein regulates platinum response in ovarian cancer-modulating SRSF2 activity

Fig. 1

a Experimental design of the loss-of-function screening. Briefly MDAH and SKOV3 cells were transduced with 2040 shRNAs targeting 680 genes in 96-well plates in duplicate and treated or not with CBDCA for 16 h. Cell viability was evaluated by MTS assay, 24 h after the end of the treatment. Statistical analyses (Z-score and adjusted p value for multiple testing) identified 50 potential targets (22 in MDAH, 24 in SKOV3, and 4 in both cell lines). The 50 genes were then validated in a subsequent screening using five shRNAs/gene in four different cell lines. Validated genes were considered genes for which at least three shRNAs increased the sensitivity to CBDCA in at least three cell lines. Using these parameters we identified SFPQ (and other four genes). Nonlinear regression analyses of cell viability in MDAH (b) and SKOV3 cells (c). Cells were transduced with control shRNA (sh-ctrl) or two different SFPQ shRNAs (sh-SFPQ#2 and #3) and treated with increasing doses of CBDCA and CDDP for 16 h. The table shows the IC50 and the confidence interval (CI) of each condition. Data are expressed as percentage of viable cells compared with untreated cells and represent the mean (±SD) of three biological replicates. Fisher’s exact test was used to calculate the global p value reported in the graph. On the right western blot (WB) analysis reporting SFPQ expression in corresponding silenced cells. Tubulin and Vinculin were used as loading control.

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