BPTF regulates growth of adult and pediatric high-grade glioma through the MYC pathway

A Correction to this article was published on 22 January 2020

Abstract

High-grade gliomas (HGG) afflict both children and adults and respond poorly to current therapies. Epigenetic regulators have a role in gliomagenesis, but a broad, functional investigation of the impact and role of specific epigenetic targets has not been undertaken. Using a two-step, in vitro/in vivo epigenomic shRNA inhibition screen, we determine the chromatin remodeler BPTF to be a key regulator of adult HGG growth. We then demonstrate that BPTF knockdown decreases HGG growth in multiple pediatric HGG models as well. BPTF appears to regulate tumor growth through cell self-renewal maintenance, and BPTF knockdown leads these glial tumors toward more neuronal characteristics. BPTF’s impact on growth is mediated through positive effects on expression of MYC and MYC pathway targets. HDAC inhibitors synergize with BPTF knockdown against HGG growth. BPTF inhibition is a promising strategy to combat HGG through epigenetic regulation of the MYC oncogenic pathway.

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Fig. 1: Justification for BPTF investigation in GBM.
Fig. 2: Validation of independent BPTF growth effect in BT 145.
Fig. 3: BPTF growth effect in other cell lines.
Fig. 4: Effect of BPTF knockdown on differentiation and cell self-renewal.
Fig. 5: BPTF knockdown interaction with MYC and downstream effectors.
Fig. 6: Potential epigenetic-targeting compounds synergistic with BPTF knockdown.

References

  1. 1.

    Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, et al. The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathologica. 2007;114:97–109.

    PubMed  PubMed Central  Google Scholar 

  2. 2.

    Wen PY, Kesari S. Malignant gliomas in adults. N Engl J Med. 2008;359:492–507. (Research support, N.I.H., extramural research support, Non-U.S. Gov’t Review).

    CAS  PubMed  Google Scholar 

  3. 3.

    Stupp R, Mason WP, van den Bent MJ, Weller M, Fisher B, Taphoorn MJ, et al. Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma. N Engl J Med. 2005;352:987–96.

    CAS  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Fangusaro J. Pediatric high-grade gliomas and diffuse intrinsic pontine gliomas. J Child Neurol. 2009;24:1409–17.

    PubMed  Google Scholar 

  5. 5.

    Rodriguez-Paredes M, Esteller M. Cancer epigenetics reaches mainstream oncology. Nat Med. 2011;17:330–9.

    CAS  PubMed  Google Scholar 

  6. 6.

    Clarke J, Penas C, Pastori C, Komotar RJ, Bregy A, Shah AH, et al. Epigenetic pathways and glioblastoma treatment. Epigenetics. 2013;8:785–95.

    CAS  PubMed  PubMed Central  Google Scholar 

  7. 7.

    Maleszewska M, Kaminska B. Is glioblastoma an epigenetic malignancy? Cancers. 2013;5:1120–39.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. 8.

    Ferreira WA, Pinheiro Ddo R, Costa Junior CA, Rodrigues-Antunes S, Araujo MD, Leao Barros MB, et al. An update on the epigenetics of glioblastomas. Epigenomics. 2016;8:1289–305.

    CAS  PubMed  Google Scholar 

  9. 9.

    Touat M, Idbaih A, Sanson M, Ligon KL. Glioblastoma targeted therapy: updated approaches from recent biological insights. Ann Oncol. 2017;28:1457–72.

    CAS  PubMed  PubMed Central  Google Scholar 

  10. 10.

    Lulla RR, Saratsis AM, Hashizume R. Mutations in chromatin machinery and pediatric high-grade glioma. Sci Adv. 2016;2:e1501354.

    PubMed  PubMed Central  Google Scholar 

  11. 11.

    Schwartzentruber J, Korshunov A, Liu XY, Jones DT, Pfaff E, Jacob K, et al. Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma. Nature. 2012;482:226–31.

    CAS  PubMed  Google Scholar 

  12. 12.

    Ghantous A, Hernandez-Vargas H, Byrnes G, Dwyer T, Herceg Z. Characterising the epigenome as a key component of the fetal exposome in evaluating in utero exposures and childhood cancer risk. Mutagenesis. 2015;30:733–42.

    CAS  PubMed  PubMed Central  Google Scholar 

  13. 13.

    Miozzo M, Vaira V, Sirchia SM. Epigenetic alterations in cancer and personalized cancer treatment. Future Oncol. 2015;11:333–48.

    CAS  PubMed  Google Scholar 

  14. 14.

    Lee P, Murphy B, Miller R, Menon V, Banik NL, Giglio P, et al. Mechanisms and clinical significance of histone deacetylase inhibitors: epigenetic glioblastoma therapy. Anticancer Res. 2015;35:615–25.

    CAS  PubMed  PubMed Central  Google Scholar 

  15. 15.

    Morales La Madrid A, Hashizume R, Kieran MW. Future clinical trials in DIPG: bringing epigenetics to the clinic. Front Oncol. 2015;5:148.

    PubMed  PubMed Central  Google Scholar 

  16. 16.

    Mottamal M, Zheng S, Huang TL, Wang G. Histone deacetylase inhibitors in clinical studies as templates for new anticancer agents. Molecules. 2015;20:3898–941.

    CAS  PubMed  PubMed Central  Google Scholar 

  17. 17.

    Herms JW, von Loewenich FD, Behnke J, Markakis E, Kretzschmar HA. c-myc oncogene family expression in glioblastoma and survival. Surg Neurol. 1999;51:536–42.

    CAS  PubMed  Google Scholar 

  18. 18.

    Wang J, Wang H, Li Z, Wu Q, Lathia JD, McLendon RE, et al. c-Myc is required for maintenance of glioma cancer stem cells. PloS ONE. 2008;3:e3769.

    PubMed  PubMed Central  Google Scholar 

  19. 19.

    Broad D DepMap Achilles 19Q1 Public. Broad Institute, Cambridge, MA, 2019.

  20. 20.

    Ghandi M, Huang FW, Jané-Valbuena J, Kryukov GV, Lo CC, McDonald ER, et al. Next-generation characterization of the Cancer Cell Line Encyclopedia. Nature. 2019;569:503–8.

    CAS  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Meyers RM, Bryan JG, McFarland JM, Weir BA, Sizemore AE, Xu H, et al. Computational correction of copy number effect improves specificity of CRISPR-Cas9 essentiality screens in cancer cells. Nat Genet. 2017;49:1779–84.

    CAS  PubMed  PubMed Central  Google Scholar 

  22. 22.

    Xie Q, Wu QL, Kim L, Miller TE, Liau BB, Mack SC, et al. RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation. J Clin Investig. 2016;126:2757–72.

    PubMed  Google Scholar 

  23. 23.

    Bao SD, Wu QL, McLendon RE, Hao YL, Shi Q, Hjelmeland AB, et al. Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Nature. 2006;444:756–60.

    CAS  PubMed  Google Scholar 

  24. 24.

    Urick AK, Hawk LM, Cassel MK, Mishra NK, Liu S, Adhikari N, et al. Dual screening of BPTF and Brd4 using protein-observed fluorine NMR uncovers new bromodomain probe molecules. ACS Chem Biol. 2015;10:2246–56.

    CAS  PubMed  PubMed Central  Google Scholar 

  25. 25.

    Li H, Ilin S, Wang W, Duncan EM, Wysocka J, Allis CD, et al. Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF. Nature. 2006;442:91–5.

    CAS  PubMed  PubMed Central  Google Scholar 

  26. 26.

    Stankiewicz P, Khan TN, Szafranski P, Slattery L, Streff H, Vetrini F, et al. Haploinsufficiency of the chromatin remodeler BPTF causes syndromic developmental and speech delay, postnatal microcephaly, and dysmorphic features. Am J Hum Genet. 2017;101:503–15.

    CAS  PubMed  PubMed Central  Google Scholar 

  27. 27.

    Bekpen C, Tastekin I, Siswara P, Akdis CA, Eichler EE. Primate segmental duplication creates novel promoters for the LRRC37 gene family within the 17q21.31 inversion polymorphism region. Genome Res. 2012;22:1050–8.

    CAS  PubMed  PubMed Central  Google Scholar 

  28. 28.

    Ma Y, Liu X, Liu Z, Wei S, Shang H, Xue Y, et al. The chromatin remodeling protein Bptf promotes posterior neuroectodermal fate by enhancing Smad2-activated wnt8a expression. J Neurosci. 2015;35:8493–506.

    CAS  PubMed  PubMed Central  Google Scholar 

  29. 29.

    Gong YC, Liu DC, Li XP, Dai SP. BPTF biomarker correlates with poor survival in human NSCLC. Eur Rev Med Pharm Sci. 2017;21:102–7.

    Google Scholar 

  30. 30.

    Buganim Y, Goldstein I, Lipson D, Milyavsky M, Polak-Charcon S, Mardoukh C, et al. A novel translocation breakpoint within the BPTF gene is associated with a pre-malignant phenotype. PloS ONE. 2010;5:e9657.

    PubMed  PubMed Central  Google Scholar 

  31. 31.

    Lee JH, Kim MS, Yoo NJ, Lee SH. BPTF, a chromatin remodeling-related gene, exhibits frameshift mutations in gastric and colorectal cancers. APMIS. 2016;124:425–7.

    CAS  PubMed  Google Scholar 

  32. 32.

    Xiao S, Liu L, Fang M, Zhou X, Peng X, Long J, et al. BPTF associated with EMT indicates negative prognosis in patients with hepatocellular carcinoma. Dig Dis Sci. 2015;60:910–8.

    CAS  PubMed  Google Scholar 

  33. 33.

    Richart L, Carrillo-de Santa Pau E, Rio-Machin A, de Andres MP, Cigudosa JC, Lobo VJ, et al. BPTF is required for c-MYC transcriptional activity and in vivo tumorigenesis. Nat Commun. 2016;7:10153.

    CAS  PubMed  PubMed Central  Google Scholar 

  34. 34.

    Dai M, Lu JJ, Guo W, Yu W, Wang Q, Tang R, et al. BPTF promotes tumor growth and predicts poor prognosis in lung adenocarcinomas. Oncotarget. 2015;6:33878–92.

    PubMed  PubMed Central  Google Scholar 

  35. 35.

    Dar AA, Nosrati M, Bezrookove V, de Semir D, Majid S, Thummala S, et al. The role of BPTF in melanoma progression and in response to BRAF-targeted therapy. J Natl Cancer Inst. 2015;107:pii:djv034.

  36. 36.

    Xiao S, Liu L, Lu X, Long J, Zhou X, Fang M. The prognostic significance of bromodomain PHD-finger transcription factor in colorectal carcinoma and association with vimentin and E-cadherin. J Cancer Res Clin Oncol. 2015;141:1465–74.

    CAS  PubMed  Google Scholar 

  37. 37.

    Frey WD, Chaudhry A, Slepicka PF, Ouellette AM, Kirberger SE, Pomerantz WCK, et al. BPTF maintains chromatin accessibility and the self-renewal capacity of mammary gland stem cells. Stem Cell Rep. 2017;9:23–31.

    CAS  Google Scholar 

  38. 38.

    Kim J, Lo L, Dormand E, Anderson DJ. SOX10 maintains multipotency and inhibits neuronal differentiation of neural crest stem cells. Neuron. 2003;38:17–31.

    CAS  PubMed  Google Scholar 

  39. 39.

    Bramanti V, Tomassoni D, Avitabile M, Amenta F, Avola R. Biomarkers of glial cell proliferation and differentiation in culture. Front Biosci. 2010;2:558–70.

    Google Scholar 

  40. 40.

    Person F, Wilczak W, Hube-Magg C, Burdelski C, Moller-Koop C, Simon R, et al. Prevalence of betaIII-tubulin (TUBB3) expression in human normal tissues and cancers. Tumour Biol. 2017;39:1010428317712166.

    PubMed  Google Scholar 

  41. 41.

    Su Z, Niu W, Liu ML, Zou Y, Zhang CL. In vivo conversion of astrocytes to neurons in the injured adult spinal cord. Nat Commun. 2014;5:3338.

    PubMed  PubMed Central  Google Scholar 

  42. 42.

    Bouchard C, Thieke K, Maier A, Saffrich R, Hanley-Hyde J, Ansorge W, et al. Direct induction of cyclin D2 by Myc contributes to cell cycle progression and sequestration of p27. EMBO J. 1999;18:5321–33.

    CAS  PubMed  PubMed Central  Google Scholar 

  43. 43.

    Kerosuo L, Piltti K, Fox H, Angers-Loustau A, Hayry V, Eilers M, et al. Myc increases self-renewal in neural progenitor cells through Miz-1. J Cell Sci. 2008;121:3941–50.

    CAS  PubMed  Google Scholar 

  44. 44.

    Nebbioso A, Carafa V, Conte M, Tambaro FP, Abbondanza C, Martens J, et al. c-Myc modulation and acetylation is a key HDAC inhibitor target in cancer. Clin Cancer Res. 2017;23:2542–55.

    CAS  PubMed  Google Scholar 

  45. 45.

    Grasso CS, Tang Y, Truffaux N, Berlow NE, Liu L, Debily MA, et al. Functionally defined therapeutic targets in diffuse intrinsic pontine glioma. Nat Med. 2015;21:827.

    CAS  PubMed  Google Scholar 

  46. 46.

    Lapin DH, Tsoli M, Ziegler DS. Genomic insights into diffuse intrinsic pontine glioma. Front Oncol. 2017;7:57.

    PubMed  PubMed Central  Google Scholar 

  47. 47.

    Landry J, Sharov AA, Piao Y, Sharova LV, Xiao H, Southon E, et al. Essential role of chromatin remodeling protein Bptf in early mouse embryos and embryonic stem cells. PLoS Genet. 2008;4:e1000241.

    PubMed  PubMed Central  Google Scholar 

  48. 48.

    Green AL, Ramkissoon SH, McCauley D, Jones K, Perry JA, Hsu JH, et al. Preclinical antitumor efficacy of selective exportin 1 inhibitors in glioblastoma. Neuro Oncol. 2015;17:697–707.

    CAS  PubMed  Google Scholar 

  49. 49.

    Baird NL, Bowlin JL, Cohrs RJ, Gilden D, Jones KL. Comparison of varicella-zoster virus RNA sequences in human neurons and fibroblasts. J Virol. 2014;88:5877–80.

    PubMed  PubMed Central  Google Scholar 

  50. 50.

    Wu TD, Nacu S. Fast and SNP-tolerant detection of complex variants and splicing in short reads. Bioinformatics. 2010;26:873–81.

    CAS  PubMed  PubMed Central  Google Scholar 

  51. 51.

    Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010;28:511–5.

    CAS  PubMed  PubMed Central  Google Scholar 

  52. 52.

    Arrigoni L, Richter AS, Betancourt E, Bruder K, Diehl S, Manke T, et al. Standardizing chromatin research: a simple and universal method for ChIP-seq. Nucleic acids Res. 2016;44:e67.

    PubMed  Google Scholar 

  53. 53.

    Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014;30:2114–20.

    CAS  PubMed  PubMed Central  Google Scholar 

  54. 54.

    Wu TD, Watanabe CK. GMAP: a genomic mapping and alignment program for mRNA and EST sequences. Bioinformatics. 2005;21:1859–75.

    CAS  PubMed  PubMed Central  Google Scholar 

  55. 55.

    Ramirez F, Ryan DP, Gruning B, Bhardwaj V, Kilpert F, Richter AS, et al. deepTools2: a next generation web server for deep-sequencing data analysis. Nucleic Acids Res. 2016;44:W160–5.

    CAS  PubMed  PubMed Central  Google Scholar 

  56. 56.

    Ross-Innes CS, Stark R, Teschendorff AE, Holmes KA, Ali HR, Dunning MJ, et al. Differential oestrogen receptor binding is associated with clinical outcome in breast cancer. Nature. 2012;481:389–93.

    CAS  PubMed  PubMed Central  Google Scholar 

  57. 57.

    Benjamini Y, Hochberg YJ. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc. 1995;B57:289–300.

    Google Scholar 

  58. 58.

    Andrade JM, Estevez-Perez MG. Statistical comparison of the slopes of two regression lines: a tutorial. Anal Chim Acta. 2014;838:1–12.

    CAS  PubMed  Google Scholar 

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Acknowledgements

We would like to thank Dr William Pomerantz for his expertize and discussion of BPTF small molecule inhibitors, and Drs Stuart Orkin, Mark Kieran, and Jay Bradner for their advice at early stages of the project. We also acknowledge the University of Colorado Denver Research Histology Shared Resource, supported by the Cancer Center Support Grant (P30CA046934), as well as the University of Colorado Anschutz Medical Campus Advanced Light Microscopy Core Facility and Functional Genomics Core Facility, for their assistance. The Colorado Animal Imaging Shared Resource is supported by the NCI and the University of Colorado Cancer Center (P30CA046934).

Funding

This work was supported by the Luke’s Army Pediatric Cancer Research Fund St. Baldrick’s Scholar Award, a Hyundai Hope on Wheels Young Investigator award, a Morgan Adams Foundation grant, a Pedals for Pediatrics grant, and NICHD K12HD068372 (ALG).

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Correspondence to Adam L. Green.

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Green, A.L., DeSisto, J., Flannery, P. et al. BPTF regulates growth of adult and pediatric high-grade glioma through the MYC pathway. Oncogene 39, 2305–2327 (2020). https://doi.org/10.1038/s41388-019-1125-7

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