Fig. 4 | Oncogene

Fig. 4

From: Initiation of human mammary cell tumorigenesis by mutant KRAS requires YAP inactivation

Fig. 4

KRAS transformation of primary human mammary cells leads to YAP inactivation in vitro. a Representative pictures from 14-day 3D cultures of human BCs and LPs transduced with YFP, YAPS127A, KRASG12D, or KRASG12D + YAPS127A. Bar graphs show clone numbers (relative to YFP control); N = 3 donors. b Western blots showing YAP phosphorylation (Ser127) levels (relative to YAP) in control and KRASG12D-transduced human BCs and LP cells assessed 3 days post transduction; bar graph showing P-YAP/YAP ratio (Actin used as internal control) in human BCs and LPs transduced with YFP, or KRASG12D; N = 3 donors. c CTGF and CYR61 mRNA levels from control and KRASG12D-transduced human BCs and LPs assessed 3 days post transduction. GAPDH mRNA was used to normalize the RNA content in each sample; N = 5 or 6 donors. P-values are from a paired t-test comparing matched starting cells transduced with KRASG12D with or without YAPS127A. d CTGF mRNA levels from BCs (left, blue) and LPs (right, red) transduced with YFP, KRASG12D, YAPS127A, or KRASG12D + YAPS127A. GAPDH mRNA was used as a normalizer for each sample. N = 3 donors

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