Table 2 IHC analysis and key genetic alterations of spheroid cultures

From: High-risk blastemal Wilms tumor can be modeled by 3D spheroid cultures in vitro

SpheroidsWT01WT02WT03WT04WT05
Life span (months)3–4167>11>36
Doubling time (weeks)22111
HistologyBlastemalBlastemal, few stromal elementsBlastemalEpithelialstromal
IHCaSphTuSphTuSphTuSphTuSphTu
SIX1/2++++++ B+++ B
+ S
+++ B
− S
++++++ B
+ S
+++++ E
+ S
++++++ S
NCAM++++++ B+++ B
+ S
+++ B
− S
+++++ B
− S
++++++ E
− S
++++ S
PAX2++++++ B+++ B
− S
++ B
− S
++++ B
− S
+++++ E
− S
− S
VIM+++++ B+++++ B
++ S
++++ B
++ S

++ S
− E
+++ S
+++++ S
CK7/8++++ B+++ B
− S
+ B
− S
++++++ E
− S
− S
Ki-6780%20%60%30%90%20%80%30%60%70%
Key genetic alterations
Mutations (SNV)GLI3 Q1437* (NM_000168:exon15:c.C4309T)MYCN T58M (NM_001293228:exon2:c.C173T), MAX R60Q (NM_002382:exon4:c.G179A)STK32C V339M (NM_001318878:exon8:c.G1015A)TP53 G245S (NM_001126112:exon7:c.G733A), CREBBP R1498* (NM_004380: exon27:c.C4492T)CTNNB1 T41A (NM_001098209:exon3:c.A121G)
CNV18p + q gainNormalDIS3L2 lossmultiple gains/losses chr.4,6,7,9,10,11,14, 16, 17, 19, 221q gain, 7q gain, WT1 loss, 11q23.2 loss
LOH (regions affected frequently in WT)11p15-p132q11-q37
11p15-p13
11p1511p15-p13, 11q13-q25, 16q13-q24.3, multiple others11p15-p13
  1. aSemiquantitative classification of staining: +++ strong, ++ moderate, + weak, − no staining
  2. Restriction to histological compartment if appropriate: B blastemal, E epithelial, S stromal elements