Fig. 5 | Oncogene

Fig. 5

From: Pathogenic mutations in neurofibromin identifies a leucine-rich domain regulating glioma cell invasiveness

Fig. 5

C-terminal region of NF1-LRD domain is required for suppressing cell invasion. a Truncation mutants used in this study. b Western blot shows the expression of the various truncation mutants as determine by immunblotting for HA. Actin serves as loading control. c Invasion assay was performed to determine the region of NF1-LRD that is necessary for suppressing cell invasion. (i) Percentage of invasion was calculated based on the number of HA+ cells vs. HA− cells followed by normalizing to vector-transfected cells. DAPI stained the nucleus. (ii) Representative images showed HA+ cells vs. HA− cells in the membrane. Data are presented as quadruplicate ± SEM. Representative result from five independent experiment was shown. Student’s unpaired t test was used statistical analysis, *p < 0.01, **p < 0.001, ***p < 0.0001. d (i) D1849N and W1952* mutations were introduced into LRD 1839–1881 and LRD 1882–1971, respectively. (ii) Western blot shows the expression of the mutants as determined by HA. Actin serves as loading control. e Effect of point mutations in the peptides LRD 1839–1881 and LRD 1882–1971 was assessed. (i) Percentage of invasion was calculated based on the number of HA + cells vs. HA− cells followed by normalizing to vector-transfected cells. (ii) Representative images showed HA+ cells vs. HA− cells in the membrane. DAPI stained the nucleus. Data are presented as quadruplicate ± SEM. Student’s unpaired t test was used statistical analysis, *p < 0.01, **p < 0.001, ***p < 0.0001