Fig. 4 | Oncogene

Fig. 4

From: Pathogenic mutations in neurofibromin identifies a leucine-rich domain regulating glioma cell invasiveness

Fig. 4

Pathogenic mutations in the NF1-LRD domain abolish suppression of invasion. a Oncomap shows the distribution of point mutations in the NF1-LRD domain. Mutations were derived from query performed on cBioportal TCGA dataset. b Invasion was assessed in neurofibromin-negative (i) LN229 and (ii) U251MG transduced with pCDH, wt-LRD, W1952* and D1849N. The percentage of invasive cells was normalized to that of pCDH vector. c Percentage of invasion was performed on neurofibromiin-negative (i) NNI-19 and (ii) NNI-24 patient-derived GPCs transduced with pCDH, wt-LRD, W1952*, and D1849N. n = 4 replicates. Immunoblot demonstrated expression of NF1-LRD as determine by HA expression. Actin serves as loading control. Densitometry quantification was performed by normalizing NF1-LRD expression to actin. Ratios were indicated below the blot. All data are presented as mean ± SEM from three independent experiment, n = 5 replicates. Student’s unpaired t test was used to analyze statistical significance between wt and mutant LRD to that of vector, *p < 0.01, **p < 0.001, ***p < 0.0001