Fig. 3 | Oncogene

Fig. 3

From: Pathogenic mutations in neurofibromin identifies a leucine-rich domain regulating glioma cell invasiveness

Fig. 3

NF1-LRD reverses EMT markers expression. The expression of a SNAI1 and ZEB2, b Vimentin, CD44, and Sox2 were assessed in NF1-shRNA-vector or NF1-shRNA-NF1-LRD-transduced NNI-21 GPCs. Data shown are fold change of mRNA expression normalized to shCtrl. Data are presented as mean ± SEM. Student’s unpaired t test was used to analyze statistical significance between NF1-LRD and vector, *p < 0.01, **p < 0.001, ***p < 0.0001. c Immunohistochemistry staining of Sox2, Vimentin, and CD44 in NF1-shRNA-vector or NF1-shRNA-NF1-LRD-transduced NNI-21 tumor. Scale bar = 50 μm. d Quantification of (i) Sox2, (ii) Vimentin, and (iii) CD44-positive cells from IHC staining. One-way ANOVA followed by Bonferroni’s post hoc test was performed to analyze statistical significance. *p < 0.01, **p < 0.001, ***p < 0.0001