Fig. 3 | Oncogene

Fig. 3

From: Estradiol induces BDNF/TrkB signaling in triple-negative breast cancer to promote brain metastases

Fig. 3

A subpopulation of cells within TNBC cell lines, PDXs, and clinical BM express TrkB. a WB shows EGFR, TrkB (140 and 110 kD isoforms), TrkC, p75NTR expression in murine (4T1BR5 and E0771) and human TNBC brain homing cells (231BR, F2-7). SKNSH and mouse brain (MB) were used as positive controls (n = 2). b Flow cytometry analysis shows the percentage of live cells expressing TrkB in 4T1BR5, F2-7 and 231BR cells. c Immunohistochemistry shows TrkB and EGFR expression in three TN BM-patient-derived xenografts. Numbers represent intensity and percentage of positive cells as scored by a pathologist. d TrkB (Green) in brain metastatic cells marked by pan cytokeratin (PanCK, red) assessed by double IF in a TNBM clinical sample. Scale bar is 100 µm. e Dots show % of tumor cells with membrane TrkB staining scored ≥ 2 + using Aperio (see supplementary Fig. 4 for detailed analysis method), in a cohort of breast cancer BM (n = 9)

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