Fig. 5 | Oncogene

Fig. 5

From: Exosome circRNA secreted from adipocytes promotes the growth of hepatocellular carcinoma by targeting deubiquitination-related USP7

Fig. 5

circ-DB regulates the miR-34a/USP7/Cyclin A2 pathway. a, b Two of the predicted binding regions of miR-34a in circ-DB. c, d Verification of the interaction between miR-34a and circ-DB by using luciferase assay (n = 3). e RT-PCR quantification of circ-DB that we constructed or the inhibition effect of circ-DB siRNA. f The level of miR-34a when cells were treated with circ-DB-OE plasmid and circ-DB siRNA. g, h The expression level of USP7 and Cyclin A2 in mature adipocytes treated with circ-DB-OE plasmid and circ-DB (n = 3). i Quantification of USP7 in 3T3L1 cells treated with circ-DB-OE plasmid and circ-DB siRNA (f) (n = 3) **p < 0.01, and ***p < 0.001, respectively

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