Fig. 3 | Oncogene

Fig. 3

From: Exosome circRNA secreted from adipocytes promotes the growth of hepatocellular carcinoma by targeting deubiquitination-related USP7

Fig. 3

Validation of USP7 as a direct target of miR-34a in HCC cells as well as targeting of Cyclin A2. a The putative binding sites for miR-34a and USP7. b RT-PCR analysis of the level of miR-34a, which we constructed (n = 3). c RT-qPCR analysis of USP7 mRNA captured by biotin-miR-34a (n = 3). d Direct recognition of USP7 by miR-34a (n = 3). Firefly luciferase reporters containing either wild-type (WT) or mutant (Mut) USP7, miR-34a mimics and the associated normal control were co-transfected into 293 T cells. The relative luciferase levels were detected after transfection (n = 3). eg WB revealed the expression levels of USP7 and cyclin A2 in HCC cells treated with miR-34a mimics or miR-34a inhibitors (n = 3). h Corresponding quantification of USP7 and cyclin A2 expression as demonstrated by RT-PCR (n = 3). i, j The expression level of Cyclin A2 when USP7 was overexpressed or inhibited by siRNA (n = 3). **p < 0.01

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