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Metabolic reprogramming by PCK1 promotes TCA cataplerosis, oxidative stress and apoptosis in liver cancer cells and suppresses hepatocellular carcinoma

Oncogenevolume 37pages16371653 (2018) | Download Citation

Abstract

Phosphoenolpyruvate carboxykinase (PEPCK or PCK) catalyzes the first rate-limiting step in hepatic gluconeogenesis pathway to maintain blood glucose levels. Mammalian cells express two PCK genes, encoding for a cytoplasmic (PCPEK-C or PCK1) and a mitochondrial (PEPCK-M or PCK2) isoforms, respectively. Increased expressions of both PCK genes are found in cancer of several organs, including colon, lung, and skin, and linked to increased anabolic metabolism and cell proliferation. Here, we report that the expressions of both PCK1 and PCK2 genes are downregulated in primary hepatocellular carcinoma (HCC) and low PCK expression was associated with poor prognosis in patients with HCC. Forced expression of either PCK1 or PCK2 in liver cancer cell lines results in severe apoptosis under the condition of glucose deprivation and suppressed liver tumorigenesis in mice. Mechanistically, we show that the pro-apoptotic effect of PCK1 requires its catalytic activity. We demonstrate that forced PCK1 expression in glucose-starved liver cancer cells induced TCA cataplerosis, leading to energy crisis and oxidative stress. Replenishing TCA intermediate α-ketoglutarate or inhibition of reactive oxygen species production blocked the cell death caused by PCK expression. Taken together, our data reveal that PCK1 is detrimental to malignant hepatocytes and suggest activating PCK1 expression as a potential treatment strategy for patients with HCC.

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Acknowledgements

We thank Dr Bin Zhao (Zhejiang University, Hangzhou, China) for providing plasmids used for inducing liver tumor in mice, including PB[CMV-myc-YAP-5SA]DS, PB[Act-RFP]DS, and Act-PB Transposase. We thank Xiaocan Guo for technical help on hydrodynamic injection. We also thank Dr Jiahuai Han (Xiamen University, Xiamen, China) for generously offering cDNAs for human PCK1 and PCK2 genes. This work was supported by the National Science and Technology Major Project of China (No. 2017ZX10203207-002-004 to HXY), the 973 Program (No. 2015CB910401 to YX), the NSFC grants (No. 31570784 and 81773190 to HXY), and NIH grants (CA163834 to YX; CA196878 and GM51586 to K-LG).

Author information

Affiliations

  1. The Molecular and Cell Biology Research Lab, Institute of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China

    • Meng-Xi Liu
    • , Si-Jia Sun
    • , Peng Liu
    • , Xu Feng
    • , Zhou-Li Cheng
    • , Kun-Liang Guan
    • , Hai-Xin Yuan
    •  & Yue Xiong
  2. Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China

    • Meng-Xi Liu
    • , Si-Jia Sun
    • , Peng Liu
    • , Xu Feng
    • , Zhou-Li Cheng
    • , Kun-Liang Guan
    • , Hai-Xin Yuan
    •  & Yue Xiong
  3. State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China

    • Meng-Xi Liu
    • , Kun-Liang Guan
    • , Hai-Xin Yuan
    •  & Yue Xiong
  4. Department of Liver Surgery, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China

    • Lei Jin
    • , Wei-Ren Liu
    •  & Ying-Hong Shi
  5. Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Shanghai, China

    • Lei Jin
    • , Wei-Ren Liu
    •  & Ying-Hong Shi
  6. Department of Pharmacology and Moores Cancer Center, University of California San Diego, La Jolla, CA, USA

    • Kun-Liang Guan
  7. Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    • Yue Xiong

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Conflict of interest

K-LG is co-founder of Vivace Therapeutics. The remaining authors declare that they have no competing interests.

Corresponding authors

Correspondence to Ying-Hong Shi or Hai-Xin Yuan or Yue Xiong.

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DOI

https://doi.org/10.1038/s41388-017-0070-6