Obsessive–compulsive disorder (OCD) is commonly associated with alterations in cortico-striato-thalamo-cortical brain networks. Yet, recent investigations of large-scale brain networks suggest that more diffuse alterations in brain connectivity may underlie its pathophysiology. Few studies have assessed functional connectivity within or between networks across the whole brain in pediatric OCD or how patterns of connectivity associate with treatment response. Resting-state functional magnetic resonance imaging scans were acquired from 25 unmedicated, treatment-naive children and adolescents with OCD (12.8 ± 2.9 years) and 23 matched healthy control (HC) participants (11.0 ± 3.3 years) before participants with OCD completed a course of cognitive-behavioral therapy (CBT). Participants were re-scanned after 12–16 weeks. Whole-brain connectomic analyses were conducted to assess baseline group differences and group-by-time interactions, corrected for multiple comparisons. Relationships between functional connectivity and OCD symptoms pre- and post-CBT were examined using longitudinal cross-lagged panel modeling. Reduced connectivity in OCD relative to HC participants was detected between default mode and task-positive network regions. Greater (less altered) connectivity between left angular gyrus and left frontal pole predicted better response to CBT in the OCD group. Altered connectivity between task-positive and task-negative networks in pediatric OCD may contribute to the impaired control over intrusive thoughts early in the illness. This is the first study to show that altered connectivity between large-scale network regions may predict response to CBT in pediatric OCD, highlighting the clinical relevance of these networks as potential circuit-based targets for the development of novel treatments.
Obsessive–compulsive disorder (OCD) is a disabling neuropsychiatric condition characterized by intrusive thoughts (obsessions) and repetitive, ritualistic behaviors (compulsions). Neuroimaging studies of OCD commonly identify structural and functional alterations in cortico-striato-thalamo-cortical (CSTC) brain circuits [1,2,3,4,5,6,7,8,9,10,11]. However, emerging evidence suggest that dysfunction in more diffuse brain networks (e.g., frontoparietal (FPN) and default mode (DMN) networks) may underlie OCD [11,12,13,14,15,16,17,18,19]. Few studies have assessed functional connectivity within or between such networks in pediatric OCD [20, 21] and none have assessed associations with treatment response. Cognitive-behavioral therapy (CBT) is an effective, first-line treatment for OCD [22,23,24,25] that shows better response than pharmacotherapy in pediatric OCD [26,27,28]. Nevertheless, treatment-resistant symptoms often remain in many patients following either type of treatment [29,30,31,32], pointing to the importance of identifying reliable biologically based predictors of response to inform clinical decisions and develop personalized treatments tailored to individual patients (i.e., precision medicine). Resting-state functional magnetic resonance imaging (rs-fMRI) is used to investigate network dynamics in normative and psychiatric populations and can identify biological predictors of clinical outcomes. Herein, we used longitudinal rs-fMRI data from children and adolescents with OCD to identify alterations in large-scale functional networks and characterize connectivity-symptom changes pre- and post-CBT. Such knowledge may ultimately improve treatment selection and foster the development of novel treatments that target brain networks more effectively.
The prevailing neurobiological model of OCD proposes that obsessive–compulsive symptoms arise from altered functioning of the CSTC circuits that support the capacity to engage control over obsessions and urges to perform rituals [33,34,35]. Indeed, structural and task-based fMRI data implicate OCD-related dysfunctions mainly in fronto-striatal portions of CSTC circuitry [5, 36,37,38,39,40,41,42,43,44,45,46]. Rs-fMRI data also suggest abnormalities in CSTC connectivity, albeit inconsistently, with findings of both hypo- [3, 8] and hyper-connectivity [2, 6, 7]. Other models suggest impairments in large-scale brain networks that extend beyond CSTC circuits, such as altered connectivity within and between FPN, salience, cingulo-opercular, dorsal and ventral attention, and DMN networks [13, 14, 47].
Discrepancies among previous rs-fMRI findings are likely owing to inconsistencies in methods and sample characteristics. For example, studies vary in their use of hypothesis-driven, seed-based connectivity versus hypothesis-free, data-driven analyses. Findings of altered CSTC connectivity stem primarily from seed-based analyses [2, 3, 6, 8], whereas large-scale network findings stem from data-driven whole-brain connectomic approaches [7, 12, 15, 18, 20, 48,49,50,51,52,53,54,55]. Among such approaches, the examination of pairwise associations (i.e., edges) between regions (i.e., nodes) across the entire brain matrix (i.e., the connectome), revealed disturbances in network connectivity in adults with OCD [7, 15, 18, 49, 54, 55]. Only one cross-sectional study used such an approach in pediatric OCD, pointing to less-efficient information transfer and differently organized networks in OCD .
In adults with OCD, CBT is associated with changes in multiple brain networks, including, but not restricted to CSTC regions . Baseline connectivity and pre- to post-CBT changes in connectivity are associated with post-CBT symptoms and pre- to post-CBT changes in symptoms [48, 49, 52, 53]. Similar results exist from task-based fMRI studies [31, 56,57,58,59]. Univariate analyses such as those used in such prior studies do not permit testing the directionality of relationships between brain measures (i.e., connectivity or activity) and symptoms, thereby precluding inferences about causality [60,61,62]. Cross-lagged panel modeling, a multivariate approach based on structural equation modeling, can be used to disentangle whether pre-treatment functional connectivity predicts CBT-related changes in OCD symptoms, whether pre-treatment symptom severity predicts changes in functional connectivity, or both (i.e., transactional relationships). This approach allows us to simultaneously test relationships between variables over time while controlling for their cross-sectional association and for the stability of each individual variable across time points. Cross-lagged associations provide a conservative test about the proportion of change in one variable (e.g., OCD symptoms) that is uniquely the result of the other variable (e.g., functional connectivity at baseline), thereby supporting inference about directionality of relationships [63,64,65].
Herein, we used a data-driven whole-brain connectomic approach to examine the functional connectivity between regions of canonical functional networks [66, 67] in a sample of treatment-naive children and adolescents with OCD. We also used cross-lagged panel modeling to examine relationships between connectivity and OCD symptoms pre- and post-CBT, allowing us to identify and disentangle directional effects. We hypothesized that functional connectivity would be altered between network regions that extend beyond CSTC circuits in pediatric OCD participants and that these alterations would normalize following CBT. We also predicted that less-altered connectivity at baseline would predict better treatment response. Given the scarcity of prior rs-fMRI studies in pediatric OCD and inconsistencies in adult OCD literature, we did not formulate specific hypotheses about the specific networks/regions in which connectivity would be altered, or the direction (i.e., hyper- versus hypo-connected) of such alterations.
Participants were 55 unmedicated and treatment-naive children and adolescents (age 7–18 years), including 28 patients with a primary diagnosis of OCD and 27 matched healthy controls (HC), group-matched for age, sex, race, and ethnicity (CONSORT flowchart in Figure S1). Participants were recruited through New York City area clinics, local, online, and radio advertisements. All participants provided informed assent and their caregiver provided informed consent. The study was approved by the Institutional Review Board at the New York State Psychiatric Institute (NYSPI) where the study was conducted between 2014 and 2017.
At baseline, a structured diagnostic interview, the Anxiety Disorders Interview Schedule for Children (ADIS) adapted for DSM-IV, child and parent versions , as well as the Wechsler Abbreviated Scale of Intelligence (WASI)  were delivered to all participants. Participants with OCD were also administered the Children’s Yale-Brown Obsessive–Compulsive Scale (CY-BOCS), present, and lifetime version [70, 71] to assess OCD symptom severity, as well as the Clinical Global Impressions (CGI) Severity of Illness and Improvement Scale  to assess global OCD symptom impression, at baseline, mid-, and end of treatment (see below). OCD patients were included if they met diagnostic criteria for OCD and had clinically significant symptoms (i.e., CY-BOCS > 16) at baseline. Other anxiety disorders (i.e., generalized anxiety disorder, panic disorder, social anxiety disorder, specific phobia, and separation anxiety) were only permitted in the OCD group if OCD was the primary diagnosis. Participants with a history of neurological disorders, past seizures, head trauma with loss of consciousness, mental retardation (i.e., WASI IQ < 80), pervasive developmental disorder, tic disorder, or current psychiatric diagnoses (other than OCD and anxiety in the patients) were excluded. HCs had no lifetime psychiatric diagnoses. Participants were re-scanned 12–16 weeks following their baseline assessment.
Following baseline assessment, patients with OCD underwent a course of manualized CBT with exposure and response prevention adapted for pediatric OCD  delivered by a licensed clinical psychologist or advanced supervised graduate student in clinical psychology at the NYSPI. CBT treatment consisted of 12–16 h-long sessions. In exceptional cases of no clinical improvement after six treatment sessions, as determined by the therapist and study team psychiatrist, complementary pharmacological treatment was offered. See supplementary information for details.
Participant characteristics and treatment outcome analyses
Using IBM SPSS (v.23), group differences in participant demographics were assessed at baseline and follow-up using independent t tests for continuous variables and chi-squared tests for categorical variables. A paired t test assessed CY-BOCS score decline pre- to post-treatment in the OCD patients.
MRI data were collected using a GE Signa 3 Tesla MR750 scanner (Milwaukee, WI), equipped with a 32-channel head coil (Nova), using a scanning protocol adapted from the Human Connectome Project (HCP) protocols . T2-weighted localizing sagittal images (2D) were acquired used to position the axial functional images parallel to anterior commissure-posterior commissure line. The following images were acquired: (1) two high-resolution T1-weighted BRAVO structural images (field of view = 240 mm, flip angle = 12°, 0.8 mm isotropic voxels, 224 slices) and a high-resolution T2-weighted image (TR = 2500 msec, TE = maximum, field of view = 256 mm, flip angle = 12°, 0.8 mm isotropic voxels, 224 slices); (2) two short spin echo, specifically echo planar imaging (EPI) sequences (TR = 5200 msec, TE = 25 msec, field of view = 192 mm, 2 mm isotropic voxels, 72 slices with no gaps) with opposite phase encoding (posterior-anterior vs. anterior-posterior) directions to measure the B0 field for EPI distortion correction; and (3) functional multi-band EPI T2*-weighted images (TR = 850 msec, TE = 25 msec, flip angle = 60°, multi-band factor = 6, field of view = 192 mm, 2 mm isotropic voxels, 66 slices with interleaved acquisition and no gaps, 7 min 42 sec, 544 volumes). Participants were instructed to stay still and keep their eyes open while viewing a fixation cross.
Twelve frames of scanner calibration data, reconstructed as two EPI volumes, were discarded prior to preprocessing. Six additional EPI volumes were discarded as the scanner reached steady state. The HCP preprocessing pipelines  v.3.4 were used to preprocess all imaging data. In brief, this included gradient unwarping, motion correction, field map-based EPI distortion correction, brain-boundary-based registration of EPI to structural T1-weighted scan, non-linear (FNIRT) registration into MNI152 space, and grand-mean intensity normalization. After this “minimal preprocessing”, all analyses were performed in the “grayordinate” space output of the pipeline (“91k” CIFTI format: 91,282 grayordinates, 32,492 per cortical hemisphere and 29,298 subcortical), which combines cortical surface and subcortical volume representations. The surface and subcortical volume data were smoothed with a 4 mm Gaussian kernel using the Workbench software (http://www.humanconnectome.org/software/connectome-workbench) developed by the HCP (wb_command -cifti-smoothing). Additional processing was run in MATLAB R2016a (Mathworks, Natick, MA). Using commands from Analysis of Functional NeuroImages (AFNI; http://afni.nimh.nih.gov/) and FMRIB Software Library (FSL; http://fsl.fmrib.ox.ac.uk/fsl/fslwiki/) software, BOLD signal was demeaned and detrended (AFNI 3dDetrend), despiked (AFNI 3dDespike -localedit), and bandpass filtered (FSL fslmaths 0.01–0.08 Hz). Nuisance regression of 24 motion parameters (bandpass filtered) and average global gray signal was performed. Motion censoring (Power et al., 2012) based on framewise displacement (FD; > 0.2 mm) and DVARS (z score > 3) was also performed.
Whole-brain connectome-level analyses
We conducted resting-state analyses on 333 cortical surface  and 19 subcortical  parcellated regions, computing rs-fMRI connectivity-strength indices using Fisher Z-Transformation of the pairwise Pearson correlation coefficients between each region. Edge-wise functional connectivity analyses were then conducted whole-brain across the resulting matrix comprised of 352 nodes and 123,904 edges. Analyses examining group differences and group-by-time interactions were adjusted for age at the time of scanning, sex, and head motion (mean FD) and conducted using the Network-Based Statistics Toolbox (v.1.2) . Both false discovery rate (FDR) and network-based statistics (NBS) methods were employed to control for type I error, using a statistical threshold p = 0.05 and 20,000 permutations. FDR tests the null hypothesis at the individual connection level and is more sensitive to focal effects, whereas NBS tests the null hypothesis at the network level based on family-wise error and is more sensitive to distributed network effects. For significant findings that converged across these two correction methods, connectivity strengths were extracted from the matrix and used in subsequent analyses to limit the number of statistical tests performed.
Cross-lagged panel modeling
Cross-lagged panel models were constructed using IBM SPSS Amos (v.23) to test for directional (“causal”) relationships between OCD symptoms and functional connectivity pre- to post-CBT in the OCD patients. These models provide estimates of the extent to which a variable pre-CBT (e.g., connectivity) predicts another variable post-CBT (e.g., CY-BOCS), over and above the variability attributable to scores of that second variable (e.g., CY-BOCS) pre-CBT . Thus, by controlling for the effects of each variable pre-CBT on the same variable post-CBT, pre-CBT variables then predict the residual, or change in that variable from pre- to post-CBT. CY-BOCS total scores at each time point were used as the OCD symptoms measure.
The initial model, depicted in Fig. 1, adjusted for age, sex, and head motion. All variables at each time point were covaried to adjust for shared variance. Non-significant paths (ps > 0.1) were removed one at a time within each model, and a chi-square difference test examined whether each removal significantly reduced model fit (order of removal and fit indices for the initial and final models are presented in the supplementary information). All stability paths were retained, even if non-significant, to adjust for baseline levels of each variable.
Effects of comorbid anxiety and medication
To ensure that group differences were not attributable to the presence of comorbid anxiety in the OCD group, we conducted ancillary between-group analyses on those edges for which group effects were detected across both FDR and NBS correction methods after excluding the OCD patients with comorbid anxiety. These ancillary analyses were conducted in IBM SPSS (v.23) using the multivariate analysis of variance command and, as in our initial whole-brain connectomic analyses, adjusted for age, sex, and head motion (i.e., mean FD). Rather than statistically adjusting for the presence of comorbid anxiety, these sensitivity analyses involved excluding participants because this variable was collinear with group (i.e., only participants in the OCD group had comorbid anxiety).
Similarly, for each cross-lagged panel model in which a significant relationship between functional connectivity and OCD symptoms pre- and post-CBT was detected, we conducted ancillary models: (1) adjusting for comorbid anxiety as a time varying covariate, and (2) excluding two patients who began taking SSRIs mid-treatment. Because these analyses were conducted in the OCD group only, the models adjusted for the presence comorbid anxiety at each time point and did not require excluding participants from the analysis.
After quality control, rs-fMRI data were available from 25 OCD and 23 HC participants at baseline and from 21 OCD and 15 HC participants at follow-up (three patients and eight HC participants dropped out from treatment/study before completion). Demographic and clinical characteristics from the final sample are shown in Table 1. No significant baseline group differences in age, sex, or head motion were detected (ps > 0.05; Table 1). Among the 21 patients who completed the treatment protocol, total CY-BOCS scores declined significantly pre- to post-treatment (t(20) = 6.230, p < 0.001), with an average decrease of 9.3 ± 6.9 points (38.6 ± 16.4%). In all, 71% (n = 15) patients responded with > 25% reduction in CY-BOCS and 52% (N = 11) with a > 35% reduction. In all, 76% (n = 16) patients had a post-treatment CY-BOCS score ≤ 12.
Whole-brain connectome-level analyses
Functional connectivity findings are summarized in Fig. 2 and Table 2. Both FDR and NBS methods revealed significant group differences in DMN-FPN and DMN-Auditory Network connectivity at baseline (N = 25 OCD and N = 23 HC). Specifically, relative to HC participants, patients with OCD exhibited reduced connectivity of left angular gyrus (parcel label default 7) with left and right rostral middle frontal gyrus (frontoparietal 9 and frontoparietal 22), and with right posterior insula (auditory 15). In addition to these findings detected with both methods, the FDR method also yielded findings of reduced connectivity in patients with OCD between other DMN regions and “task-positive” network regions that are typically engaged during attention-demanding tasks (i.e., ventral and dorsal attention, and cingulo-opercular networks) [79,80,81,82], and between visual network regions (detailed in Table 2), as well as increased connectivity in OCD relative to HC participants was detected in between right putamen and posterior insula (part of the auditory network). No significant group-by-time interactions were detected with either FDR or NBS method (Ns = 21 OCD, 15 HC).
Cross-lagged panel modeling
Separate cross-lagged panel models were computed in the OCD group (N = 25) for the three edges that differed significantly across groups at baseline with both FDR and NBS (i.e., left angular gyrus (default 7) to left and right rostral middle frontal gyrus (frontoparietal 9 and 22), and to right posterior insula (auditory15)). Descriptive statistics and bivariate correlations between outcome variables are presented in Table S1. Figures 3 and S1 present the final models with significant connectivity-symptom associations pre- and post-CBT. Based on a Bonferroni correction for the three models tested, ps < 0.017 were considered significant and corrected. Greater (less reduced) left default 7-to-frontoparietal 9 (DMN-to-left FPN) connectivity at baseline predicted greater reduction in OCD symptoms at follow-up (B = −0.521, p < 0.001; Fig. 3). In addition, less-severe OCD symptoms at baseline predicted greater increase in default 7-to-Auditory 15 (left DMN-to-right auditory) connectivity at follow-up (B = −0.580, p = 0.012; Figure S2). In no instance did the removal of a non-significant path result in significant model fit reduction; thus, no non-significant path (other than stability paths) was retained in the final model. Detailed statistics and goodness of fit for the initial and final models are reported in the supplementary information and Table S2.
Effects of comorbid anxiety and medication
Baseline group differences in connectivity remained significant after excluding patients with comorbid anxiety (F(3,24) = 8.473, p = 0.001; Ns = 8 OCD, 23 HC). Relationships between functional connectivity and OCD symptoms pre- and post-CBT also remained significant after adjusting for comorbid anxiety in our cross-lagged panel models (Table S3; N = 25) and after excluding two patients who began receiving complementary SSRI medication mid-treatment (Table S4; N = 23).
Using a data-driven whole-brain connectomic approach, we identified altered functional connectivity between regions of canonical large-scale brain networks in pediatric OCD that also predicted response to CBT. Specifically, compared with their healthy counterparts, youth with OCD exhibited reduced connectivity between regions of the DMN and regions within several task-positive networks, including FPN, ventral attention, and cingulo-opercular. Increased connectivity between right putamen and posterior insula (auditory network) was also detected. In addition, default 7-to-frontoparietal 9 connectivity at baseline associated inversely with OCD symptoms post-CBT, adjusting for baseline symptoms. These findings suggest that less-altered connectivity between these regions predicts better response to CBT in pediatric OCD.
Our findings of altered connectivity between task-positive and DMN regions are in line with meta-analytic findings showing consistent hypoconnectivity within and between FPN, salience, and DMN in adults and adolescents with OCD . The salience network defined in that and other prior studies is comprised of insular cortex, ACC, supramarginal gyrus, and largely overlaps with the ventral attention and cingulo-opercular networks defined in the brain parcellation used herein . Although these task-positive networks are comprised of regions engaged during goal-directed tasks requiring attention and cognitive control [79,80,81,82], DMN regions are typically engaged during rest [83,84,85,86,87,88,89], mind wandering, and self-referential processes [90,91,92]. Our findings of altered connectivity between task-positive and DMN regions may suggest a network imbalance, consistent with a triple network model , whereby the salience network interfaces between the DMN and FPN, acting like a switch modulating the attention and cognitive resources between self-referential thoughts, internal processes (i.e., DMN processes) and external goal-directed behavior (i.e., FPN processes). Imbalance across this triple network may underlie OCD , contributing to difficulty disengaging from obsessive thoughts and/or urges to perform compulsive behaviors in the service of more adaptive goal-directed behavior.
Cross-lagged panel modeling revealed that default 7-to-frontoparietal 9 connectivity significantly predicted response to CBT, such that more (i.e., less reduced) connectivity was associated with greater reduction in OCD symptoms. Prior graph theory findings suggested reduced network segregation between the DMN and FPN in adults with OCD that normalized following SSRI treatment . Although altered connectivity in the present study of children and adolescents did not normalize with CBT, both sets of findings suggest that altered DMN-FPN connectivity may mark the illness and serve as a target for symptom change. Other data from adults with OCD suggest that patterns of connectivity within the DMN significantly predicted response to CBT. Between-network connectivity was not assessed in that prior study, however, thereby precluding direct comparison between those findings and ours.
Previous findings from a small sample of youth with OCD (N = 15, 80% medicated) suggested reduced connectivity between left putamen and a cluster of regions in the salience/cingulo-opercular network, including a portion of the anterior insula . In contrast, we detected increased connectivity between right putamen and right posterior insula, which is part of a different network (i.e., auditory). Findings from another small sample (N = 14) suggested increased connectivity between bilateral putamen and left-sided clusters encompassing posterior insula and surrounding cortical regions following SSRI treatment, with increased left putamen connectivity associated with clinical improvement . Whereas these previous studies used a seed-based approach, we used a data-driven approach and rigorous correction methods—only exploring further those findings of group differences that survived both NBS and FDR corrections—precluding further comparison between these prior findings and ours. Nevertheless, these extant findings together suggest that network connectivity extending beyond CSTC circuits may be an important mediator or mechanism of symptom change, underscoring the clinical relevance of these networks as potential circuit-based targets for the development of novel treatments for pediatric OCD.
This is the first fMRI study to examine the effects of CBT on functional connectivity and relationships with symptom change in youth with OCD. Our data-driven connectomic approach permitted the identification of relevant markers that predict treatment response in large-scale brain networks, extending outside the classical CSTC circuits. We used stringent methods of multiple comparison correction to minimize the risk of type I error and carefully controlled for potential confounding effects, including age, sex, motion, and comorbid anxiety and SSRI augmentation in the OCD group, rendering our analyses quite conservative. Our cross-lagged panel models adjusted for the independent effects of these potential confounds as well as baseline levels of each predicted outcome variable (i.e., functional connectivity and OCD symptoms) in addition to the covariance between variables at each time point. These sophisticated longitudinal analyses thus allowed us to disentangle connectivity-symptom relationships pre- and post-CBT, thereby permitting inference about the directional influence of DMN-FPN connectivity on CBT-related change in OCD symptoms. Finally, all participants were medication-free and treatment-naive at baseline, received protocol driven CBT with excellent response, and potential effects of mid-treatment SSRI initiation in two patients were ruled out in ancillary analyses.
Notwithstanding these notable strengths, our modest sample size limited power to detect effects and precluded examining specific effects of, for example, different OCD symptom dimensions [95,96,97], comorbidities, or changes in functional connectivity pre-to-post CBT across the entire brain while preserving adequate stringency. As such, our study may have failed to detect several relevant effects that should be investigated in future multi-site studies of much larger pediatric OCD samples. Relatedly, altered connectivity at baseline in the OCD relative to HC participants did not significantly normalize with CBT, as suggested by our lack of group-by-time interactions in our whole-brain connectomic analyses. However, these findings should be interpreted with caution given the modest number of HC participants with follow-up data (N = 15 HC), which rendered these analyses underpowered to detect effects. Inclusion of OCD participants with comorbid anxiety disorders is another limitation, however exploratory analyses revealed that this comorbidity did not contribute to our findings of altered connectivity and its prediction of CBT response in pediatric OCD. Although we employed a longitudinal design that permitted the examination of the directionality of relationships between symptoms and functional connectivity pre- and post-CBT, the collection of symptom and connectivity data along more time points would have allowed examination of how connectivity predicts long-term remission or relapse. Another limitation is that there was no placebo control group (e.g., OCD patients undergoing relaxation therapy) or a waitlist group. It may be that the relationships between functional connectivity and symptom change were not specific to CBT per se. Finally, a limitation inherent to our connectomic approach is the lack of any gold standard for large-scale brain parcellation, resulting in node definition being somewhat arbitrary and inconsistent across studies . All available parcellation methods have respective advantages and limitations: coarser parcellations typically comprise functionally heterogeneous regions and may fail to detect true effects. Alternatively, finer parcellations (e.g., treating each voxel as a separate node) may fail to account for the large homogeneity across neighboring nodes and result in noisy and underpowered estimates. In the present study, we used a parcellation scheme developed based on the recent technological advances in MRI acquisition, with greater parcel homogeneity than alternative parcellations and reliable mapping of known architectonic areas and connectivity patterns across individual participants .
In conclusion, this study is first to employ a data-driven whole-brain connectomic approach to examine network connectivity in pediatric OCD before and after a full course of CBT treatment. Our innovative analytical approach combined with high temporal resolution fMRI data allowed to identify abnormalities between networks that extend outside the classical CSTC circuits. Cross-lagged panel modeling of longitudinal data allowed to disentangle the directionality of the relationships between functional connectivity and OCD symptoms in response to CBT. Our findings point to altered task-positive and task-negative networks connectivity in pediatric OCD that may play a key role in patient’s ability to benefit from CBT. Future longitudinal studies of large samples should replicate and extend these findings that may ultimately lead to treatment targets for future circuit-based interventions.
Funding and disclosure
This work was supported by a grant from the National Institute of Mental Health (R21MH101441; MPIs: Marsh and Rynn) and by a fellowship from the Fonds de Recherche du Quebec, Sante (Fellow: Cyr). The study was registered with clinicaltrials.gov (NCT02421315). Dr. Moira Rynn receives an honorarium as a DSMB member from Allergan Inc. All other authors declare no competing interests.
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Cyr, M., Pagliaccio, D., Yanes-Lukin, P. et al. Altered network connectivity predicts response to cognitive-behavioral therapy in pediatric obsessive–compulsive disorder. Neuropsychopharmacol. 45, 1232–1240 (2020). https://doi.org/10.1038/s41386-020-0613-3
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