Dopaminergic modulation of corticostriatal transmission is critically involved in reward-driven behaviors. This modulation is mainly mediated by dopamine D1 receptors (D1Rs) and D2Rs, which are highly expressed in medium spiny neurons (MSNs) of the dorsomedial striatum (DMS), a brain region essential for goal-directed behaviors and addiction. D1Rs and D2Rs are also present at presynaptic cortical terminals within the DMS. However, it is not known how addictive substances alter the glutamatergic strength of striatal synapses expressing presynaptic dopamine receptors. Using cell type-specific Cre mice in combination with optogenetic techniques, we measured glutamatergic transmission at D1R- or D2R-expressing afferents to DMS MSNs. We found larger excitatory postsynaptic currents at the synapses between the extra-striatal D2R-expressing afferents and D1R-expressing MSNs (D2→D1), as compared with those observed at the other tested synapses (D1→D1, D1→D2, and D2→D2). Additionally, excessive alcohol consumption induced a long-lasting potentiation of glutamatergic transmission at the corticostriatal D2→D1 synapse. Furthermore, we demonstrated that activation of postsynaptic, but not presynaptic, D2Rs inhibited corticostriatal transmission in an endocannabinoid-dependent manner. Taken together, these data provide detailed information on the mechanisms underlying dopamine receptor-mediated modulation of brain reward circuitry.
Dopamine signaling in the corticostriatal circuit is centrally involved in reward-driven behaviors . By acting on different glutamatergic terminals, striatal dopamine regulates specific excitatory inputs from the cortex and thalamus onto medium spiny neurons (MSNs), contributing to the selection of appropriate behaviors . Dopaminergic modulation is mainly mediated by dopamine D1 receptors (D1Rs) and D2Rs [2, 3], which are highly expressed in the dorsomedial striatum (DMS), a brain region essential for goal-directed behaviors and addiction [4, 5]. DMS MSNs express D1Rs or D2Rs  and anatomical evidence indicates that these receptors are present at presynaptic cortical terminals in the striatum [7, 8]. These presynaptic receptors facilitate dopamine-mediated regulation of excitatory signaling to specific striatal pathways . Altered dopaminergic transmission is a common mechanism underlying drug and alcohol abuse [9, 10]. However, it is not known how excessive alcohol consumption affects glutamatergic synapses expressing particular pre- and postsynaptic dopamine receptors.
The medial prefrontal cortex (mPFC) afferent input to the DMS has been extensively implicated in drug and alcohol addiction [11,12,13]. Excessive alcohol exposure has been reported to enhance glutamatergic responses at mPFC inputs to the DMS , and this enhancement was recently shown to contribute to a long-lasting enhancement of alcohol-seeking and alcohol-taking behaviors . The neuronal subtypes within the mPFC can also be distinguished based on their expression of D1Rs and D2Rs . However, it is not known how excessive alcohol consumption distinctly alters D1R- or D2R-expressing mPFC afferents onto striatal MSNs.
In this study, we measured the effects of excessive alcohol intake on glutamatergic transmission at DMS synapses expressing different pre- and postsynaptic dopamine receptors. We discovered that synapses containing presynaptic D2Rs and postsynaptic D1Rs (D2→D1) exhibited stronger glutamatergic connectivity than the other tested synapses (D1→D1, D1→D2, and D2→D2). Excessive alcohol intake induced a long-lasting potentiation of glutamatergic transmission at the corticostriatal D2→D1 synapse. In addition, we demonstrated that D2R-mediated inhibition of glutamatergic transmission in the DMS is mediated by distinct pre- and postsynaptic mechanisms. These findings contribute to the elucidation of the detailed mechanisms underlying the dopaminergic modulation of different brain reward circuitry. Our results also identified alcohol-evoked circuit-specific plasticity in the DMS, which may contribute to excessive alcohol consumption.
Materials and methods
Details are provided in the Supplemental Materials and Methods.
The viral vector was purchased from the University of North Carolina Vector Core. Reagents were obtained from Sigma, R&D Systems, and Abcam.
The D1-Cre and D2-Cre mice were obtained from Mutant Mouse Regional Resource Centers. Ai32, Ai14, and Snap25 mice were purchased from the Jackson Laboratory. All animal care and experimental procedures were approved by the Texas A&M University Institutional Animal Care and Use Committee.
AAV-Flex-Chronos-GFP or AAV-CAG-Flex-GFP was bilaterally infused into the mPFC (AP, +1.94 mm; ML, ±0.25 mm; DV, −2.5 mm from the Bregma) of D1-/D2-Cre;Ai14 mice. AAVretro-CAG-Tdtomato was bilaterally infused into the DMS (AP1, +1.18 mm; ML1, ±1.3 mm; DV1, −2.9 mm; AP2, +0.38 mm; ML2, ±1.67 mm; DV2, −2.9 mm from the Bregma) of D1-/D2-Cre;Snap25 mice.
Intermittent-access to 20% alcohol two-bottle-choice drinking procedure
Slice preparation and electrophysiological recordings have been described previously [2, 5, 15, 16, 18]. Slices were maintained in external solution containing (in mM): 125 NaCl, 4.5 KCl, 2.5 CaCl2, 1.3 MgCl2, 1.25 NaH2PO4, 25 NaHCO3, 15 glucose, and 15 sucrose. The electrodes contained (in mM): 119 CsMeSO4, 8 TEA.Cl, 15 HEPES, 0.6 EGTA, 0.3 Na3GTP, 4 MgATP, 5 QX-314, and 7 phosphocreatine (for voltage-clamp recording) or 123 potassium gluconate, 10 HEPES, 0.2 EGTA, 8 NaCl, 2 MgATP, 0.3 NaGTP (for current-clamp recording). A GABAA receptor antagonist, picrotoxin (100 μM), was included in the external solution for all recordings, in order to block GABAergic transmission.
Histology and cell counting
Fluorescent images were acquired using a confocal microscope (FluoView 1200, Olympus, Tokyo, Japan) and analyzed using IMARIS 8.3.1 (Bitplane, Zürich, Switzerland), as previously reported .
Data were analyzed by two-tailed t test (unpaired or paired), one-way analysis of variance (ANOVA), or two-way ANOVA with repeated measures (two-way RM ANOVA), followed by the Student-Newman-Keuls (SNK) post hoc test.
DMS glutamatergic synapses containing different pre- and postsynaptic dopamine receptors exhibit distinct connectivity
To achieve selective activation of D1R- or D2R-expressing neurons, we crossed D1-Cre or D2-Cre mice, in which Cre expression was driven by the D1R or D2R promotor, with a channelrhodopsin-2 (ChR2)-EYFP Cre reporter line, Ai32 [19, 20]. We recently verified that Cre expression in these mouse lines reliably represented D1R- and D2R-expressing neurons outside the striatum . In D1-Cre;Ai32 and D2-Cre;Ai32 mice, we observed intense expression of EYFP in the dorsal striatum and at the projection targets of D1-MSNs and D2-MSNs (Fig. 1a, b), confirming ChR2 expression in these neurons. We utilized a biophysical approach to identify MSN subtypes. In D1-Cre;Ai32 mice, D1-MSNs expressed ChR2 and responded distinctly to 2-ms and 500-ms light stimulations (Figure S1Ai, S1B) . Conversely, D2-MSNs in these animals did not express ChR2, but received glutamatergic afferents expressing D1Rs and ChR2; in these cells, 500-ms light stimulation did not significantly alter the width of the response to 2-ms light stimulation (Figure S1Aii, S1B). The same approach was used to identify D1-MSNs and D2-MSNs in D2-Cre;Ai32 mice (Figure S1C).
Next, we measured the light-evoked response in the presence of an AMPAR antagonist, DNQX (10 µM), and found that the responses at D1→D2 and D2→D1 synapses were completely blocked (Figure S1D, S1E). In contrast, the light-evoked responses of D1→D1 or D2→D2 were partially suppressed due to a large postsynaptic ChR2-mediated depolarization (Figure S1F). This ChR2-mediated direct depolarization may cause a space-clamp error, which affects the accuracy of measurements of D1→D1 and D2→D2 connectivity. To overcome this space-clamp issue, we measured the strontium (Sr2+)-induced asynchronous excitatory postsynaptic currents (aEPSCs) evoked by light stimulation [22, 23]. We found that the aEPSC frequency was significantly higher at the D2→D1 synapse than at the other three synapse types (Fig. 1c, d; F(3,49) = 32.88, p < 0.001), and that the aEPSC amplitude was identical in these four types of synapse (Fig. 1c, e; F(3,49) = 0.14, p > 0.05).
The higher aEPSC frequency indicated that the D2→D1 synapse may show the strongest connectivity. To confirm this, we compared the light-evoked AMPAR-EPSCs at D1→D2 and D2→D1 synapses; these can be measured directly, without interference from postsynaptic ChR2-mediated depolarization (Fig. 1f). We found significantly larger EPSC amplitudes at D2→D1 synapses than at D1→D2 synapses (Fig. 1g; t(16) = −2.543, p < 0.05). Lastly, we confirmed that D1-Cre and D2-Cre mice crossed with reporter lines expressed the same proportions of D1R- and D2R-expressing neurons (Figure S2).
Together, these results suggest that DMS glutamatergic synapses containing different pre- and postsynaptic dopamine receptors exhibit distinct connectivity, and that the D2→D1 connectivity is stronger than that of the other tested connections.
DMS-projecting extra-striatal neurons preferentially express D2Rs
We next explored the potential mechanism underlying the relatively greater strength of the DMS D2→D1 synapse. Previous anatomical studies indicated that cortical fibers in the dorsal striatum contain abundant D2Rs, and fewer D1Rs [7, 8]. It was therefore possible that there were more D2R-expressing fibers, as compared with those expressing the D1R. To test this, we employed D1-Cre;Snap25 and D2-Cre;Snap25 mice where D1R- and D2R-expressing neurons expressed GFP, respectively (Fig. 2a). A retrograde virus encoding tdTomato (AAVretro-CAG-tdTomato) was infused into the DMS in order to label DMS-projecting neurons (Fig. 2a). Co-expression of tdTomato and GFP was visualized as a yellow signal (Fig. 2a–d).
We observed that more of the mPFC projections to the DMS expressed the D2R, as compared with the D1R (Fig. 2b). We counted yellow neurons in other extra-striatal areas of both hemispheres throughout the entire brain. To compensate for infusion and expression variations, we normalized the number of yellow neurons to the number of red neurons in the mPFC. This analysis of brain-wide inputs to the DMS found that significantly more DMS-projecting neurons expressed the D2R, as compared with the total number of neurons that expressed the D1R (Fig. 2e; t(4) = −3.03, p < 0.05). Additionally, many individual brain sections contained significantly more D2R-expressing neurons than D1R-expressing cells (Fig. 2f; F(1,130) = 8.92, p < 0.05). Interestingly, we discovered that the posterior brain regions tended to have more D2R- than D1R-expressing DMS-projecting neurons (Fig. 2g; t(4) = −4.03, p < 0.05), while the anterior regions showed no significant differences between the D1R-expressing and D2R-expressing cells (Fig. 2g; t(4) = −2.04, p > 0.05). These data suggest that extra-striatal D2R-expressing inputs to the DMS are more prevalent than D1R-expressing inputs, and that presynaptic D2Rs may therefore play a stronger regulatory role than D1Rs.
Suppression of glutamatergic transmission at distinct DMS synapses via pre- and postsynaptic D2Rs
D2R-mediated inhibition plays a critical role in modulating corticostriatal glutamatergic transmission. Although the mechanisms underlying this effect have not been fully elucidated, they may involve the activation of presynaptic D2Rs on the glutamatergic terminal  and retrograde endocannabinoid (eCB) signaling via striatal postsynaptic D2Rs . To investigate the effect of presynaptic D2Rs on cortical inputs, we took advantage of the absence of postsynaptic D2R expression at D2→D1 synapses (Fig. 3a). We used paired-pulsed optical stimulation delivered at a frequency of 20 Hz [24, 25] to activate D2R-expressing afferents, and recorded light-evoked responses in DMS D1-MSNs of D2-Cre;Ai32 mice before and during the bath application of a D2R agonist, quinpirole (20 µM). We observed a significant inhibition of the first EPSC by quinpirole (Fig. 3b; 79.56 ± 3.78% of baseline; t(8) = 5.41, p < 0.001). Moreover, this inhibition was accompanied by a significant increase in the paired-pulse ratio (PPR) (Fig. 3c; baseline, 0.27 ± 0.03; quinpirole, 0.39 ± 0.04; t(8) = −6.46, p < 0.001), suggesting that quinpirole inhibited EPSCs by decreasing presynaptic glutamate release . We next tested whether the quinpirole-mediated inhibition required eCB signaling by treating DMS slices with AM251, a cannabinoid type 1 receptor (CB1R) antagonist. With 3 µM AM251 in the bath, quinpirole still inhibited EPSCs (Fig. 3d; EPSC 1 = 76.43 ± 5.91% of baseline; t(6) = 3.99, p < 0.01). AM251 also failed to prevent the quinpirole-induced increase in the PPR (Fig. 3e; baseline, 0.29 ± 0.03; quinpirole, 0.43 ± 0.04; t(6) = −8.75, p < 0.001). These results indicate that presynaptic D2R signaling suppresses glutamatergic transmission in an eCB-independent manner.
To determine the role of postsynaptic D2Rs on cortical inputs, we took advantage of the lack of presynaptic D2R expression at the D1→D2 synapse (Fig. 3f). We recorded D2-MSNs of D1-Cre;Ai32 mice, performed the same experiment as in Fig. 3a–e, and observed a similar inhibitory effect of quinpirole. The first EPSC amplitude was significantly reduced after quinpirole application (Fig. 3g; 72.18 ± 2.38% of baseline; t(8) = 11.67, p < 0.001), and PPR was significantly increased (Fig. 3h; baseline, 0.26 ± 0.04; quinpirole, 0.34 ± 0.05; t(8) = −4.73, p < 0.01). We next tested whether postsynaptic D2R-mediated inhibition required retrograde eCB signaling. In the presence of AM251, quinpirole failed to inhibit D1→D2 EPSCs (Fig. 3i; 99.7 ± 4.43% of baseline; t(8) = 0.07, p > 0.05) or to change PPR (Fig. 3j; baseline, 0.27 ± 0.05; quinpirole, 0.28 ± 0.05; t(8) = −1.64, p > 0.05), indicating that CB1R activation is necessary for postsynaptic D2R-mediated synaptic depression. Taken together, these results suggest that D2R-mediated inhibition of glutamatergic transmission in the DMS involves distinct pre- and postsynaptic mechanisms, and that postsynaptic D2R-mediated inhibition requires eCB signaling.
Excessive alcohol consumption causes a long-lasting potentiation of glutamatergic transmission at the DMS D2→D1 synapse
Altered dopaminergic transmission is the common mechanism underlying drug and alcohol abuse. To investigate how excessive alcohol intake alters D2→D1 and D1→D2 synaptic transmission, D2-Cre;Ai32 and D1-Cre;Ai32 mice were trained to consume 20% alcohol for 8 weeks using the intermittent-access two-bottle choice drinking procedure [2, 5, 16, 17]. As shown in Table S1, these mice consumed high levels of alcohol, and the drinking levels were identical between the two mouse lines (Table S1; q = 0.58, p > 0.05). The water control mice underwent the same treatment, but without alcohol exposure. Twenty-four hours after the last alcohol exposure, we prepared DMS slices and measured light-evoked AMPAR-mediated EPSCs in MSNs. We found that the amplitude of D2→D1 EPSCs was significantly higher in the alcohol-drinking group than in the water controls (Fig. 4a; F(1,84) = 10.01, p < 0.01). Additionally, excessive alcohol intake produced a long-lasting (9 days) increase in AMPAR-EPSCs (Fig. 4a; F(1,100) = 7.93, p < 0.01). In contrast, the amplitude of D1→D2 EPSCs was identical in both alcohol and water groups (Fig. 4b; F(1,116) = 0.57, p > 0.05). Interestingly, we also discovered that excessive alcohol intake caused a long-lasting decrease in PPR at the D2→D1 synapse (Fig. 4c; 24-h withdrawal, t(21) = 2.432, p < 0.05; 9-d withdrawal, t(26) = 2.648, p < 0.05); this effect was not observed at the D1→D2 connection (Fig. 4d; t(22) = −0.318, p > 0.05). Since the PPR is inversely correlated with transmitter release probability , this reduction in PPR indicated that the alcohol-induced enhancement of D2→D1 connectivity was mediated, at least in part, by an increased glutamate release. Collectively, these data demonstrate that excessive alcohol consumption persistently enhances glutamatergic transmission at the D2→D1 synapse of the DMS.
In the striatum, D2Rs are also expressed in cholinergic interneurons (CINs) and dopaminergic fibers [6, 27], leading to ChR2 expression by these cells in D2-Cre;Ai32 mice. Glutamate , acetylcholine, and dopamine might be released during light stimulation. However, we found that glutamatergic transmission from CINs to MSNs represented a small proportion of the overall D2→D1 transmission (Figure S3A-S3C), suggesting that most D2→D1 synapses involved extra-striatal neurons. The light-evoked release of acetylcholine and dopamine was unlikely to influence the observed glutamatergic D2→D1 transmission (Figure S3D-S3E).
The strong mPFC D2R-expressing input onto DMS D1-MSNs is potentiated by excessive alcohol consumption
The data shown in Fig. 1 indicated that glutamatergic transmission from brain-wide D2R-expressing afferents onto D1-MSNs was stronger than that observed at the other tested connections. However, we also wished to investigate the strength of D2R-expressing afferents from the mPFC onto this neuronal type. Additionally, we recently found that excessive alcohol consumption increased AMPAR activity at the mPFC input to the DMS . To access the D2R-expressing mPFC input, a Cre-dependent Chronos-expressing AAV  was infused into the mPFC of D2-Cre;Ai14 mice (Fig. 5a), which express Cre and tdTomato in D2-MSNs and in mPFC D2R-expressing neurons [2, 5]. The expression of Chronos was indicated by the presence of GFP, which was restricted selectively to D2R-expressing (red) mPFC neurons (Fig. 5b). Infusion of AAV-Flex-Chronos-GFP caused intense green fluorescent labeling of mPFC D2R-expressing afferents in the DMS (Fig. 5c). DMS D2-MSNs were identified by the red fluorescence of tdTomato (Fig. 5c) and any non-fluorescent striatal MSNs were therefore considered as putative D1-MSNs. Whole-cell recordings were performed from these two types of MSN. Similar to the D2→D1 connection in D2-Cre;Ai32 mice, bath application of DNQX (10 µM) and an NMDAR antagonist (APV, 50 µM) completely abolished light-evoked activity at the mPFC D2R-expressing inputs onto both DMS D1-MSNs (Fig. 5d) and D2-MSNs (Fig. 5e), suggesting that they were glutamatergic. To compare the glutamatergic strength of D2→D1 and D2→D2 connections, we measured light-evoked AMPAR-EPSCs in D2-Cre;Ai14 mice. We found that the amplitudes of AMPAR-EPSCs were significantly higher in the D2→D1 group than in the D2→D2 group (Fig. 5f; F(1,112) = 4.95, p < 0.05). These results indicate that mPFC D2R-expressing inputs distinctly control D1-MSNs and D2-MSNs, with a stronger glutamatergic connection between mPFC D2R-expressing neurons and DMS D1-MSNs.
Lastly, we explored the effect of excessive alcohol intake on AMPAR-mediated EPSCs at mPFC D2R-expressing inputs onto DMS D1- or D2-MSNs. D2-Cre;Ai14 mice with mPFC infusion of AAV-Flex-Chronos-GFP were trained to consume high levels of alcohol (Table S1), as described above. Twenty-four hours after the last alcohol exposure, we prepared DMS slices and measured light-evoked EPSCs in DMS D1-MSNs and D2-MSNs. We found that excessive alcohol intake selectively potentiated AMPAR-EPSCs at D2R-expressing mPFC inputs onto DMS D1-MSNs (Fig. 5g; F(1,112) = 8.36, p < 0.01). In contrast, excessive alcohol intake did not alter EPSCs at D2R-expressing mPFC inputs onto DMS D2-MSNs (Fig. 5h; F(1,112) = 0.46, p > 0.05). We also investigated whether excessive alcohol intake altered presynaptic glutamate release. A marginally lower PPR was observed at the D2→D1 synapse of the mPFC-DMS pathway in alcohol-drinking mice, as compared with the water controls (Fig. 5i; t(28) = 1.77, p = 0.087). However, the PPR at the D2→D2 synapse did not differ between the alcohol and water groups (Fig. 5i; t(28) = −0.22, p > 0.05). These data suggest that excessive alcohol intake may increase the probability of glutamate release selectively at the mPFC D2R-expressing afferents onto D1-MSNs. In summary, these results suggest that excessive alcohol intake enhances AMPAR-mediated glutamate transmission selectively at the D2→D1 synapse within the mPFC-DMS pathway.
The present study dissected glutamatergic afferents to the striatum based on their dopamine receptor expression, and revealed that the glutamatergic connection between presynaptic D2R-expressing afferents and DMS D1-MSNs was stronger than that of the other three tested connections. This uneven synaptic strength may result in differential dopaminergic modulation of glutamatergic transmission. Additionally, we discovered that postsynaptic D2R-mediated suppression of glutamatergic transmission required eCB signaling, whereas presynaptic D2Rs mediated eCB-independent suppression. Our results contribute to the clarification of previous controversial findings relating to the role of the D2R in inhibitory presynaptic filtering of cortical inputs. Lastly, we found that excessive alcohol intake selectively strengthened glutamatergic transmission at the D2→D1 synapse, suggesting that this circuit may play a critical role in the pathogenesis of alcohol use disorder.
Glutamatergic connectivity at DMS synapses with distinct pre- and postsynaptic dopamine receptors
Dopamine is a key player in brain reward circuitry . Investigations of cortical inputs onto MSNs have demonstrated that a major role of dopamine is to promote transient shifts in the balance between the direct and indirect striatal pathways of the basal ganglia during reward-driven behaviors [1, 30]. Modulation of presynaptic filtering by dopamine may be critical in the determination of which cortical and thalamic signals are transduced by the appropriate excitatory striatal synapses during learning. Since D1Rs and D2Rs are major dopamine receptors in the corticostriatal pathway, we employed D1-Cre;Ai32 and D2-Cre;Ai32 mice to dissect the circuitry into at least four types of synapse: D1→D1, D1→D2, D2→D1, and D2→D2.
In both brain-wide and mPFC studies, we found that glutamatergic D2→D1 transmission was stronger than that observed at the tested connection. It is known that in the dorsal striatum, D2Rs are more prevalent than D1Rs presynaptically at corticostriatal projections [1, 7, 8]. This may explain our findings indicating that D2R-expressing inputs were stronger than D1R-expressing afferents in the DMS. However, our observation that the D2→D1 connection was stronger than the D2→D2 synapse indicated a difference at the postsynaptic site. Midbrain dopamine neurons predominantly fire at a low frequency, and such tonic activity builds up the basal level of dopamine in the striatum . This tonic dopamine inhibits D2-MSNs via postsynaptic D2Rs. When the glutamatergic and dopaminergic inputs are activated at high frequency, eCB is preferentially generated in the D2-MSNs; this eCB suppresses presynaptic glutamate release onto D2-MSNs . Additionally, at the behavioral level, the striatal circuit plays a dual role in the modulation of action initiated by the cortical inputs, by reinforcing the currently selected action via the direct pathway and suppressing potentially conflicting actions via the indirect pathway . Therefore, the intrinsically stronger D2→D1 circuit (as compared with the D2→D2 synapse) in the DMS may provide a mechanism for processing information through dopamine-dependent activation of the specific direct pathway in order to procure a reward. The current study only investigated four types of dopamine receptor-based pathways. Although neurons expressing D1Rs or D2Rs were highly segregated in the cortical and striatal regions, some co-localization was observed [2, 5]. Co-expressed D1Rs and D2Rs can form heterodimers, which contribute to addiction and schizophrenia . Due to the limitations of our transgenic animals, the influence of this subpopulation on the corticostriatal pathway remains to be determined.
Alcohol-evoked circuit-specific plasticity
Accumulating evidence suggests that exposure to addictive substances, including alcohol, potentiates AMPAR-mediated responses in D1-MSNs, but not D2-MSNs [5, 16, 33]. Our current findings were consistent with this, with no alcohol-related changes observed in D2-MSNs. Importantly, D1-MSNs with inputs from extra-striatal neurons expressing D2Rs, such as mPFC D2-neurons, did exhibit marked alterations after repeated alcohol exposure. It is not fully clear why chronic alcohol exposure selectively potentiates the glutamatergic connectivity of D2→D1, while not affecting other synapses. Our observation of a decreased PPR indicated that this potentiation was mediated, at least in part, by an increase in presynaptic glutamate release. Presynaptic terminals are known to express D2Rs , and their activation by tonic dopamine is reported to inhibit glutamate release [25, 34]. The basal dopamine level is reported to decline gradually after a prolonged period of excessive alcohol drinking and withdrawal cycles [35, 36]. The D2R is a high-affinity dopamine receptor that is classically assumed to inhibit neuronal activity via Gαi protein or β-arrestin . Activation of D2R also blocks excitatory currents by recruiting Akt-GSK3 signaling . The decreased basal dopamine level may reduce D2R-mediated suppression of D2→D1 signaling, which may consequently enhance glutamatergic transmission within this pathway and thus contribute to excessive drinking behavior.
D1-Cre and D2-Cre mouse lines
To achieve specific stimulation of extra-striatal inputs, we used D1-Cre and D2-Cre lines crossed with an optogenetic reporter mouse line. One limitation of this approach is that the representation of D1R-expressing neurons in the D1-Cre mouse and of D2R-expressing neurons in D2-Cre mouse might not be equally reliable. However, we compared the Cre efficiency of D1-Cre and D2-Cre mice, and the results suggested that these mouse lines showed equivalent levels of dopamine receptor expression. Thus, the observed difference in glutamatergic strength is highly likely to relate to intrinsic differences in the pathways, rather than to Cre line efficiency differences in D1-Cre and D2-Cre mice.
In conclusion, we demonstrated that inputs to the DMS can be classified in a cell type-specific manner and that the strengths of their glutamatergic connections with two types of MSN were not identical. The connection between corticostriatal D2R-expressing inputs and D1-MSNs was stronger than that of the other three tested connections, and was selectively potentiated by excessive alcohol intake. The elucidation of this alcohol-evoked circuit-specific plasticity could contribute to the identification of new neuronal therapeutic targets for the treatment of alcohol use disorder.
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Funding And Disclosure
This research was funded by R01AA021505 (JW), U01AA025932 (JW), and the Texas Research Society on Alcoholism (JL).
Conflict of interests
The authors declare no competing interests.
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Lu, J., Cheng, Y., Wang, X. et al. Alcohol intake enhances glutamatergic transmission from D2 receptor-expressing afferents onto D1 receptor-expressing medium spiny neurons in the dorsomedial striatum. Neuropsychopharmacol. 44, 1123–1131 (2019). https://doi.org/10.1038/s41386-019-0332-9