Tight junctions are essential for barrier integrity, inflammation, and cancer. Vitamin D and the vitamin D receptor (VDR) play important roles in colorectal cancer (CRC). Using the human CRC database, we found colonic VDR expression was low and significantly correlated with a reduction of Claudin-5 mRNA and protein. In the colon of VDRΔIEC mice, deletion of intestinal VDR led to lower protein and mRNA levels of Claudin-5. Intestinal permeability was increased in the VDR−/− colon cancer model. Lacking VDR and a reduction of Claudin-5 are associated with an increased number of tumors in the VDR−/− and VDRΔIEC mice. Furthermore, gain and loss functional studies have identified CLDN-5 as a downstream target of VDR. We identified the Vitamin D response element (VDRE) binding sites in a reporter system showed that VDRE in the Claudin-5 promoter is required for vitamin D3-induced Claudin-5 expression. Conditional epithelial VDR overexpression protected against the loss of Claudin-5 in response to inflammation and tumorigenesis in vivo. We also reported fecal VDR reduction in a colon cancer model. This study advances the understanding of how VDR regulates intestinal barrier functions in tumorigenesis and the possibility for identifying new biomarker and therapeutic targets to restore VDR-dependent functions in CRC.
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We would like to thank Dr. David Zhou for assisting with the CRC human samples, Drs. Shaoping Wu and Rong Lu for assisting with the AOM/DSS model, and Jason S. Xia for proofreading.
This research was funded by the UIC Cancer Center, the NIDDK/National Institutes of Health grant R01 DK105118 and R01DK114126, VA Merit Award VA 1 I01 BX004824-01, and DOD BC160450P1 to Jun Sun. The study sponsors played no role in the study design, data collection, analysis, and interpretation of data. The contents do not represent the views of the United States Department of Veterans Affairs or the United States Government.
The authors declare no competing interests. The funders played no role in the study design, the collection, analyses, or interpretation of data, the writing of the paper, or the decision to publish the results.
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Zhang, Y., Garrett, S., Carroll, R.E. et al. Vitamin D receptor upregulates tight junction protein claudin-5 against colitis-associated tumorigenesis. Mucosal Immunol 15, 683–697 (2022). https://doi.org/10.1038/s41385-022-00502-1