Fig. 5: Testicular γδ17 promotes testis surveillance against Listeria monocytogenes. | Mucosal Immunology

Fig. 5: Testicular γδ17 promotes testis surveillance against Listeria monocytogenes.

From: Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance

Fig. 5

a, b Scatter plots show frequencies and numbers of γδ and αβ T cells among CD3+CD45+ cells after intra-testicular infection with L. monocytogenes (L. mon) (2 × 103 CFU) (white) or PBS (black) in mature testis (n = 10–13, three independent experiments) (a) and spleen (n = 5–8, two independent experiments) (b). c Representative contour plots depicting IL-17+ or IFN-γ+ γδ (left) and αβ (right) T cells 3 days after intra-testicular injection of PBS (top) or L. monocytogenes (bottom). Scatter plots show frequencies of IFN-γ+ (white) and IL-17+ (black) γδ (top) and αβ (bottom) T cells (n = 10–13, three independent experiments). d Pie charts displaying the Vγ1+, Vγ4+ and Vγ1Vγ4 usage of IL-17+ γδ (top) and IFN-γ+ (bottom) γδ T cells 3 days after intra-testicular injection of L. mon (n = 8, two independent experiments). e Scatter plots show numbers of IFN-γ+ CD4 (left), CD8 (middle) and γδ (right) T cells in mature testis of WT (black), Tcrδ/− (grey) and Il17−/− (white) mice after intra-testicular infection with L. monocytogenes (2 × 103 CFU) or PBS (n = 8–19, three independent experiments). f Survival curve of WT (white), Ifnγ−/− (red), Il17−/ (white) and Tcrδ/− (grey) mice after intra-testicular injection of L. monocytogenes (2 × 103 CFU) (n = 6–7, three independent experiments). g Bacterial burden (CFU per testis) of WT (black), Tcrδ/− (grey) and Il17/− (white) mice analysed 72 h after intra-testicular injection of L. mon (2 × 103 CFU) (n = 11–12, three independent experiments). h Survival curve of WT (white), Il17−/− (black) and Tcrδ/− (grey) mice after intra-testicular injection of L. monocytogenes (4 × 103 CFU) (n = 5–7). Data pooled are represented as mean ± SD as evaluated by Kruskal–Wallis test followed by Dunn’s multiple-comparison test or one-way ANOVA followed by Holm–Sidak’s multiple-comparison test. *P < 0.05, **P < 0.01. i Bacterial burden (CFU per testis) of WT (black), Tcrδ–/– (grey) and Il17−/− (white) mice analysed 48 h after intra-testicular injection of L. monocytogenes (4 × 103 CFU) (n = 3–5 animals/group). When possible, Tcrδ+/+ and Il17+/+ littermate controls were used and referred as WT animals.

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