Fig. 3: Accumulation of γδ T cells in the testis is dependent on microbiota, IL-23 and TLR4 signalling. | Mucosal Immunology

Fig. 3: Accumulation of γδ T cells in the testis is dependent on microbiota, IL-23 and TLR4 signalling.

From: Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance

Fig. 3

a Representative contour plots depicting IL-17-producing γδ T cells gated on CD3+CD45+ cells in testes of specific pathogen-free (SPF) (left) and germ-free (GF) (right) mice. Scatter plot shows frequencies of γδ and γδ17 T cells among lymphocytes in testes of SPF (black) and GF (white) mice (n = 18–22, five independent experiments). b Number of testicular γδ and γδ17 cells of SPF (black) and GF (white) mice (n = 11–12, three independent experiments). c Representative contour plots depicting testicular Vγ6+ γδ T cells gated on CD3+CD45+ cells of SPF and GF mice. Scatter plot displays frequencies of Vγ6+ γδ T cells among all T cells in testes of SPF (black) and GF (white) mice (n = 11–14, four independent experiments). d Number of Vγ6+ γδ T cells from SPF and GF in pre- and post-pubertal mice (n = 6, two independent experiments) (n = 7–8, three independent experiments). e Scatter plot shows frequencies of γδ17 T cells among lymphocytes of WT (black), Tlr2−/−, Tlr4−/− and Myd88−/− (white) mice (n = 4–9, one to two independent experiments). f Representative histogram of γδ (dark grey) and αβ (white) T cells expressing IL-1 receptor (R) in WT mice (left) and IL-23R in Il23rgfp/gfp mice (right) and scatter plot with mean fluorescence intensity (MFI) (n = 5–10, one to three independent experiments). g Scatter plot depicts frequencies of γδ17 among all T cells in testes of WT (black), Il1r−/−, Il23r−/−, Il1α−/− and Il1β/− (white) (n = 5–29, one to three independent experiments). h Scatter plot displays picogram (pg) per mg protein of IL-1α, IL-1β and IL-23 in the testis (n = 9, two independent experiments). Data are represented as mean ± SD as evaluated by Kruskal–Wallis test followed by Dunn’s multiple-comparison test or one-way ANOVA followed by Holm–Sidak’s multiple-comparison test. **P < 0.01, ***P < 0.001, ****P < 0.0001.

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