Fig. 2: Vγ6+ IL-17+ γδ T cells accumulate specifically in the testis during puberty. | Mucosal Immunology

Fig. 2: Vγ6+ IL-17+ γδ T cells accumulate specifically in the testis during puberty.

From: Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance

Fig. 2

a Representative contour plots depicting testicular γδ and αβ T cells gated on CD3+CD45+ cells before puberty (3–5-week-old (wo) mice) and post puberty (7–12-week-old mice). Scatter plot shows frequencies of γδ T cells among CD3+CD45+ cells in pre-pubertal (white) and post-pubertal (black) mice (n = 32–36, seven independent experiments). b Number (#) of γδ T cells in pre- (white) and post-pubertal (black) mice (n = 22–27, five independent experiments). c Representative contour plots depicting Vγ6+ γδ T cells before and after puberty. Scatter plot shows frequencies of Vγ6+ γδ T cells among lymphocytes in pre-pubertal (white) and post-pubertal (black) mice (n = 14–19, three independent experiments). d Number of Vγ6+ γδ T cells in pre- (white) and post-pubertal (black) mice (n = 14, three independent experiments). e Representative contour plots depicting IL-17-producing γδ T (γδ17) cells before and after puberty. Scatter plot shows frequencies of γδ17 cells in pre-pubertal (white) and post-pubertal (black) mice (n = 8–9, two independent experiments). f Number of γδ17 cells in pre- (white) and post-pubertal (black) mice (n = 8, two independent experiments). g Representative histogram of CCR6 expression of γδ in pre- (white) and post-pubertal (dark grey) mice. Scatter plot shows frequencies of CCR6+ γδ in pre-pubertal (white) and post-pubertal (black) mice (n = 8–9, two independent experiments). h Representative histogram of Ki67 expression of Vγ6+ γδ T cells in pre- (white) and post-pubertal (dark grey) mice (n = 7–9, three independent experiments). Data are represented as mean ± SD as evaluated by unpaired Student’s t test, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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