Fig. 1: Testicular γδ T cells display a typical phenotype biased for IL-17 production. | Mucosal Immunology

Fig. 1: Testicular γδ T cells display a typical phenotype biased for IL-17 production.

From: Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance

Fig. 1

a Representative contour plots depicting γδ (middle) and CD4 and CD8 αβ (right) T cells gated on CD3+CD45+ cells (left) in testes of C57BL/6 mice (8–12 weeks old). Scatter plot shows frequencies and absolute numbers of γδ, CD4 and CD8 T cells among CD45+CD3+ cells (n = 5–16, two to five independent experiments). b Representative histogram of CD69, CD44 and CD62L expression of γδ (dark grey) and αβ (white) T cells. Scatter plot displays frequencies of indicated cell populations among γδ (black) and αβ (white) T cells (n = 8–17, two to five independent experiments). c Representative contour plot and pie chart depicting mean frequencies of Vγ1 + , Vγ4+, Vγ5+ and Vγ6+ γδ T cells in testes (n = 6–12, three independent experiments). d Scatter plot shows frequencies of αβ and γδ T cells in testes of WT (black) and Indu-Rag1×TcrdH2BeGFP (white) mice (n = 5–9, three independent experiments). e Representative contour plot and scatter plot of RORγt and T-bet expression in testicular γδ (left, black) and αβ (right, white) T cells (n = 5, one independent experiment). f Representative histogram of CD27 expression on γδ (grey) and αβ (white) T cells. Scatter plot with frequencies of CD27+ cells among γδ (black) and αβ (white) T cells (n = 6, two independent experiments). g Representative contour plot and scatter plot of IL-17 versus IFN-γ expression in testicular γδ (left, black) and αβ (right, white) T cells (n = 20–21, five independent experiments) after ex vivo stimulation of testicular lymphocytes with PMA and ionomycin. h Representative contour plot and scatter plot of IL-17 versus IL-22 expression in IL-17GFP/IL-22-BFP reporter mice, without prior stimulation by PMA and ionomycin (n = 3–6). i Pie chart depicting indicated immune cell subsets contributing to IL-17 (left) or IFN-γ (right) production in the testis after ex vivo stimulation by PMA and ionomycin (n = 12, three independent experiments). Data are represented as mean ± SD.

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