Fig. 2 | Mucosal Immunology

Fig. 2

From: Spliceosome protein Eftud2 promotes colitis-associated tumorigenesis by modulating inflammatory response of macrophage

Fig. 2

Myeloid Eftud2 deficiency suppresses inflammatory colon tumorigenesis. a, b On day 100 after CAC induction, tumor number in colon and rectum of MKO and WT mice was counted (a). Tumor burden was determined by calculating the volume of tumor mass (b). Eight pairs of mice were examined. c, d On day 100 after CAC induction, the percentage of cases with either no adenoma, low-grade dysplasia (LGD), and high-grade dysplasia (HGD) was determined (c). H&E staining was performed (d). Eight pairs of mice were examined. An example is shown. Magnification: upper panel: ×100; lower panel: ×400. e On day 100 following CAC induction, the colonic tissues of MKO and WT mice were dissected. Proliferation and apoptosis of colonic epithelial cells was detected by Ki67 and cleaved caspase 3 staining, respectively. Five pairs of mice were examined. An example is shown on the left and the summarized results on the right. Magnification: ×200. f On day 100 after CAC induction, the colonic tissues of MKO and WT mice were dissected and CEC were isolated as described in Methods. Bcl-XL contents in CEC were determined by Western blotting. Each lane represents one mouse. ***P < 0.001

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