Fig. 6 | Mucosal Immunology

Fig. 6

From: Sex-associated TSLP-induced immune alterations following early-life RSV infection leads to enhanced allergic disease

Fig. 6

Deletion of TSLP receptor leads to decreased immune responses following CRA exposure in male mice with early-life RSV infection. Male and Female TSLPR−/− mice were infected with RSV at 7 days of age and secondary allergen challenge initiated at 4 weeks post-infection. a Lungs were embedded in paraffin and Periodic acid-Schiff stain (PAS) was performed to visualize mucus (bright pink staining). Representative photos shows b AHR was determined using full-body plethysmography and methacholine challenge (N ≥ 3). c Subjective mucus scoring was performed on blinded histological slides on a scale of 1–4 for mucus production (N ≥ 6). d, e Lungs were homogenized and mRNA extracted to determine mucus and cytokine gene expression compared to age/sex-matched naïve controls (N ≥ 3). fh Lungs were processed into single-cell suspension and stained for flow cytometry analysis (N ≥ 3). i Lung draining lymph nodes in single-cell suspension were re-stimulated with CRA in vitro for 48 h to determine cytokine protein levels (N ≥ 3). Data represent mean ± SEM (representative of or pooled from 2 to 3 individual experiments). *p < 0.05; **p < 0.01; ***p < 0.001

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