Fig. 5 | Mucosal Immunology

Fig. 5

From: Sex-associated TSLP-induced immune alterations following early-life RSV infection leads to enhanced allergic disease

Fig. 5

Early-life RSV infection leads to exacerbated allergic response in male mice. Male and Female mice were infected with RSV at 7 days of age and secondary allergen challenge initiated at 4 weeks post-infection. a Experimental Design. b Lungs were embedded in paraffin and Periodic acid-Schiff stain (PAS) was performed to visualize mucus (bright pink staining). Representative photos shown c. AHR was determined using full-body plethysmography and methacholine challenge (N ≥ 3). d Subjective mucus scoring was performed on blinded histological slides on a scale of 1–4 for mucus production (N ≥ 8). e Lungs were homogenized and mRNA extracted to determine mucus and cytokine gene expression compared to age/sex-matched naïve controls (N ≥ 3). f, g Lungs were processed into single-cell suspension and stained for flow cytometry analysis. (N ≥ 4). h Lung draining lymph nodes were processed into single cell suspension and re-stimulated with CRA in vitro for 48 h to determine cytokine protein levels (N ≥ 3). Data represent mean ± SEM (representative of or pooled from 2 to 3 individual experiments). *p < 0.05; **p < 0.01; ***p < 0.001

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