Fig. 3 | Mucosal Immunology

Fig. 3

From: Sex-associated TSLP-induced immune alterations following early-life RSV infection leads to enhanced allergic disease

Fig. 3

Male mice infected with RSV during early-life show signs of lung immunopathology that persists for up to 4 weeks post-infection. Male and Female mice were infected with RSV at 7 days of age and tissues collected at 4 weeks post-infection to evaluate long-term alterations. a, b Lungs were embedded in paraffin and Periodic acid-Schiff stain (PAS) was performed to visualize mucus (bright pink staining). Representative photos shown ce. Lungs were homogenized and mRNA extracted to determine gene expression compared to age/sex-matched uninfected controls (N = ≥ 9) fk. RNAscope was performed to determine location of Tslp and Il33 within male lungs (stained in green). Representative photos shown. Data represent mean ± SEM (representative of or pooled from 2 individual experiments). *p < 0.05; **p < 0.01; ***p < 0.001

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