Fig. 2 | Mucosal Immunology

Fig. 2

From: Sex-associated TSLP-induced immune alterations following early-life RSV infection leads to enhanced allergic disease

Fig. 2

Neonatal RSV infection of male and female mice leads to a strong Th2/Th17-type immune response with delayed resolution in male mice. Male and Female mice were infected with RSV at 7 days of age and tissues collected at 2, 4, 8, or 14 days post-infection to evaluate primary response and resolution of Th2/Th17 response. a, b Lungs were embedded in paraffin and Periodic acid-Schiff stain (PAS) was performed to visualize mucus (bright pink staining) representative photos shown. c, d Lungs were homogenized and mRNA extracted to determine RSV F and interferon-β gene expression, respectively (N ≥ 3). e, f Lung draining lymph nodes were processed into single-cell suspension and re-stimulated with RSV in vitro for 48 h to determine cytokine protein levels (N ≥ 4). Data represent mean ± SEM (representative of at least 2 individual experiments). *p < 0.05

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