Tissue-resident memory T cells (TRMs) are a novel nonvascular memory T cell subset. Although CD8+ TRMs are well-characterized, CD4+ TRMs—especially lung-resident memory Th17 cells—are still being defined. In this study, we characterized lung-resident memory Th17 cells (lung TRM17) and their role in protection against the highly virulent fungus Cryptococcus gattii. We found that intravenously transferred DCs preferentially migrated to lungs and attracted recipient DCs and led to the induction of long-lived Th17 cells expressing characteristic markers. This population could be clearly discriminated from circulating T cells by intravascular staining and was not depleted by the immunosuppressive agent FTY720. The C. gattii antigen re-stimulation assay revealed that vaccine-induced lung Th17 cells produced IL-17A but not IFNγ. The DC vaccine significantly increased IL-17A production and suppressed fungal burden in the lungs and improved the survival of mice infected with C. gattii. This protective effect was significantly reduced in the IL-17A knockout (KO) mice, but not in the FTY720-treated mice. The protective effect also coincided with the activation of neutrophils and multinucleated giant cells, and these inflammatory responses were suppressed in the vaccinated IL-17A KO mice. Overall, these data demonstrated that the systemic DC vaccine induced lung TRM17, which played a substantial role in anti-fungal immunity.
Tissue-resident memory T cells (TRMs) are a novel subpopulation of memory T cells residing in a variety of non-lymphoid peripheral tissues including the gut, lung, skin, genital tract, salivary gland, liver, and the brain1. Because they are typically located at barrier frontlines, they are thought to be involved in protection against the pathogens including viruses, bacteria, and parasites. However, several types of TRMs, including Th2-type TRMs, appear to participate in inflammatory diseases including asthma and colitis2. Therefore, modulating the behavior of TRMs is potentially important in controlling infections and inflammatory diseases. Similar to TRMs, tumor-infiltrating lymphocytes (TILs) also reside in tumor tissues, and cytotoxic T lymphocytes (CTLs)-and related TILs have the ability to improve the prognosis. Adoptive transfer of TILs is also expected to suppress growing tumors3.
TRMs can be discriminated from circulating lymphocytes and characteristically express CD69, a receptor of myosin light chain 9 (Myl9). Myl9 is secreted from platelets concomitantly with local inflammation, and forms a net-like structure (Myl9 net) on the endothelial surface. CD69-expressing activated lymphocytes can become anchored in blood vessels in a CD69- and Myl9-dependent manner and infiltrate tissues4. Further, expression of CD69 can downregulate the sphingosine-1-phosphate receptor (S1PR), which is essential for transport across blood vessel walls5. Similar to TRMs, TILs also express TRM-like markers, including CD69 and CD103 (αEβ7 integrin)3.
CD8+ TRMs are well-characterized, but less is known about CD4+ TRMs, especially Th17-type TRMs in the lungs. A memory Th17 cell-inducing vaccine is especially awaited for respiratory infections including tuberculosis, pneumococcal infection, pertussis, and flu6. Therefore, lung-resident memory Th17 cells could be an important target and a key player for vaccine development.
In a previous study, we developed a dendritic cell-based vaccine (DC vaccine) against life-threatening pulmonary cryptococcosis caused by the highly virulent pathogenic fungus Cryptococcus gattii7. An outbreak of C. gattii infection occurred in North America during 1999, and cryptococcosis caused by C. gattii is perceived as an emerging infectious disease. The highly virulent C. gattii can infect healthy individuals, and the mortality rate has been 8–20%8. Public health authorities in British Columbia and the USA have brought this situation to the public’s attention9. However, a clinical vaccine against cryptococcosis has not yet been developed, and protective memory against C. gattii infection has not been demonstrated.
DC vaccines are widely utilized in cancer therapy10 and infection control11, as well as in basic research to analyze T-cell response12. The DC vaccine that we have developed comprises mouse bone marrow-derived DCs pulsed with heat-inactivated acapsular C. gattii cells, in which the capsule synthesis-related gene CAP60 has been disrupted using a reverse genetics approach. When the DC vaccine was intravenously transferred into a murine host, we observed induction of cytokine-producing T cells in the lungs and amelioration of pulmonary cryptococcosis caused by C. gattii7. However, induction of memory T cells and any long-term protective effect conferred by the DC vaccine remained to be elucidated.
In the current study, we investigated memory T cells induced by the DC vaccine that contributed to its long-term protective effect. We also evaluated the migration of transferred DCs, distribution of C. gattii-specific memory T cells, and longevity and protective effect of the memory T cells. We demonstrate that the systemic DC vaccine induced lung-resident memory Th17 cells, which participated in protective inflammatory responses including neutrophil activation and granuloma formation in pulmonary cryptococcosis caused by a highly virulent C. gattii strain.
Antigen-pulsed DCs preferentially accumulated in the lungs after intravenous transfer
Initially, we evaluated migration of transferred DCs and activation of recipient DCs. Donor DCs were prepared from C57BL/6 (CD45.1) mice, and then intravenously transferred into recipient C57BL/6 (CD45.2) mice (Fig. 1a). After 18 h following the transfer, the antigen-pulsed DCs preferentially accumulated in the lungs, compared with spleen and mediastinal lymph node (MedLN) (Fig. 1b). Recipient DCs (CD45.2+ Ly6Glow Siglec-Flow CD11c+ I-Ab+ cells) significantly increased in the lungs of the mice that had received the antigen-pulsed DCs, but not in their spleen and MedLN (Fig. 1c), suggesting that the antigen-pulsed DC migrates to the lungs after the intravenous transfer and attracts recipient DCs.
The systemic DC vaccine induced lung-resident memory Th17 cells (lung TRM17)
Next, we evaluated memory T cells in the lungs, spleen, and bone marrow (BM) after intravenous immunization with the antigen-pulsed DCs (Fig. 2a). Memory T cells were categorized as previously reported13. More than14 weeks after the final immunization, CD4+ T cells expressing the TRM-marker CD69 were significantly increased in the lungs of the immunized mice. In contrast, this cell population was not increased in the spleen and BM of the immunized mice (Fig. 2a). Further, the DC vaccine also induced CD8+ T-cells expressing tissue-resident markers in the lungs, but to a lesser extent than their CD4+ counterparts (Supplementary Figure S1A). Vaccine-induced CD4+ T cells expressing TRM-profiles were detected in the lungs for at least 6 months after vaccination (Supplementary Figure S2). These results suggest that the DC vaccine induces long-lived CD4+ TRMs in the lungs, despite intravenous administration.
In general, TRMs immediately produced cytokines after antigen re-stimulation1,13. Therefore, we evaluated cytokine production of vaccine-induced T cells after polyclonal re-stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin (Fig. 2b). IL-17A-producing CD4+ T cells significantly increased in the lungs >10 weeks after the final vaccination (Fig. 2b). This increase was clearly higher than that in the spleen and BM, and that in lung IL-17A+ CD8+ T-cells (Supplementary Figure S1B). However, the vaccine did not induce IFNγ-producing T cells (Fig. 2b and Supplementary Figure S1B). These results suggest that the vaccine induces Th17-type TRMs in the lungs.
Next, we investigated how donor DCs were responsible for the generation of lung CD4+ TRMs (Fig. 2c). In this experiment, we utilized antigen-pulsed DCs pretreated by the major histocompatibility complex class-II (MHC-II) blocking antibody (M5/114.15.2, may also be referred to as M5/114 in the literature). This blocking antibody has been previously used in in vivo studies14,15. This blocking experiment revealed that the number of lung CD4+ TEMs and TRMs significantly decreased in the mice that received the MHC-II-blocked DC vaccine; however, the MHC-II-blocked DC vaccine still had the ability to induce CD4+ TRMs in the lungs (Fig. 2c), suggesting that MHC-II on donor DCs is partially required for the generation of lung CD4+ TRMs, and that the attracted recipient DCs are also involved in the development of lung CD4+ TRMs.
Because it has been shown that TRMs are not depleted by the administration of the drug FTY720 (fingolimod) and not stained by intravascular (IV) staining16, we tested the effect of FTY720 administration and IV staining in CD4+ TRMs. We confirmed that vascular CD4+ T cells were clearly depleted by FTY720 treatment in this model and were completely stained by IV staining (Fig. 3a). Similarly, vaccine-induced lung CD69+ CD4+ TRMs were also not depleted by FTY720 and not labeled by IV staining, and the number of CD45iv− CD69+ CD4+ TRMs (approximately 6 × 104 cells/lung) was still significantly greater than that in the counterpart of non-vaccinated controls after the FTY720 treatment (Fig. 3b). We also found that this population constitutively expressed the Th17-defining transcriptional factor RORγt but not T-bet (Fig. 3b and Supplementary Figure S3A). The vaccine-induced lung CD4+ TRMs also expressed Ki-67, CD127, CXCR3, TCF-7, Sca1, and CD43 (Supplementary Figures S3B and S3C). Histological analysis revealed that vaccine-induced CD4+ TRMs localized in the narrow space around airways and near blood vessels (Fig. 3c), which is consistent with the localization of flu-induced lung CD4+ TRMs17. These results also suggest that the DC vaccine induces lung CD4+ TRMs.
We also investigated antigen-specific memory of CD4+ TRMs against C. gattii (Fig. 4a, b). After FTY720 treatment to deplete vascular lymphocytes as described in Fig. 3, CD4+ T cells were stimulated by antigen-pulsed BMDCs (Fig. 4a). Vaccine-induced lung CD4+ TRMs produced IL-17A, but not IFNγ after in vitro re-stimulation, and vaccine-induced spleen CD4+ T cells also produced IL-17A after re-stimulation, but its population ratio was lesser than that in the lung counterparts (Fig. 4a). Because we used the heat-inactivated acapsular C. gattii strain CAP60∆ as an antigen to stimulate CD4+ T cells in this experiment (Fig. 4a), we next investigated whether the capsular C. gattii strain and non-related pathogenic fungus Candida albicans also stimulated vaccine-induced memory T cells (Fig. 4b). The heat-inactivated capsular C. gattii strain R265 also induced immunized lungs, but not immunized spleen, to secrete IL-17A production; however, heat-inactivated C. albicans SC5314 did not induce IL-17A secretion (Fig. 4b). These results suggest that vaccine-induced lung CD4+ TRMs are memory Th17 cells that respond to C. gattii-derived antigens.
Taken together, these results indicate that the systemic DC vaccine induces lung-resident memory Th17 cells, and that these cells are maintained for at least 6 months after immunization. Hereafter, we refer to this population as lung TRM17.
IL-17A was required for the generation and maintenance of vaccine-induced lung TRM17
A previous study showed that lung CD103+ CD8+ TRMs were helped by CD4+ T cells in an IFNγ-dependent manner18, but the key cytokine for generation or maintenance of lung CD4+ TRMs remained to be elucidated. We showed that vaccine-induced lung TRM17 could produce IL-17A without antigen re-stimulation and that they expressed the IL-17A receptor IL-17RA (Figs. 4 and 5a, b). These features of lung TRM17 have raised the possibility that IL-17A is a key factor for the generation or maintenance of lung TRM17. Thus, we measured the amount of lung TRM17 in the wild-type (WT) C57BL/6 and IL-17A-knockout (KO) mice 2 or 15 weeks after the final vaccination (Fig. 5c). At the primary time point, lung TRM17 modestly increased in the IL-17A-KO mice that had received the DC vaccine; however, the amount of lung TRM17 in the IL-17A KO mice was significantly lower than that in the WT mice. At the memory time point, the amount of lung TRM17 in the IL-17A KO mice decreased to non-vaccinated baseline, and CD127 expression on lung CD4+ TEMs was also significantly attenuated in them (Fig. 5c). These results suggest that IL-17A is involved in both the development and maintenance of vaccine-mediated lung TRM17 in this model.
Lung TRM17 was involved in the vaccine-mediated protective effect against highly virulent C. gattii infection
Our previous study showed that the DC vaccine induced protective immunity against highly virulent C. gattii when infected soon after the last immunization7. However, the long-term protective effect was not evaluated. Therefore, we tested the long-term protective effect of the DC vaccine in the murine pulmonary infection model. The infection challenge was performed after >2 months from the last immunization (Fig. 6a). Fungal burden at day 14 post-infection was significantly lower in the lungs of the immunized mice, even if the infection challenge was performed after 6 months from the last immunization (Fig. 6a). Amounts of IL-17A and IFNγ were significantly higher in the lungs of the immunized mice than in those of non-vaccinated controls (Fig. 6a). Furthermore, survival was also significantly improved in the immunized mice (Fig. 6b). These results suggest the long-term protective effect of the DC vaccine, and long-term protective effect appeared to be correlated to long-term retention of lung TRM17.
Next, we tested whether lung TRM17 contributed to the long-term protective effect of the DC vaccine. As described above, both the generation and maintenance of lung TRM17 were significantly reduced in the IL-17A KO mice (Fig. 5), and the effector function of lung TRM17 also appeared to be depressed in these mice. Indeed, in the IL-17A KO mice, the DC vaccine did not suppress fungal burden in the lungs at day 14 post-infection (Fig. 6c). Furthermore, depletion of circulating lymphocytes did not disturb vaccine-mediated fungal reduction in lungs (Fig. 6d, e), suggesting that vaccine-mediated protection depends on tissue-resident immunity. These results suggest that lung TRM17 contributes to the vaccine-mediated protective effect against highly virulent C. gattii.
Induction of neutrophils and granuloma depended on IL-17A in the lungs of the immunized mice after the infection challenge
Next, we examined the role of vaccine-induced lung TRM17 after the infection challenge. In this analysis, we used WT and IL-17A KO mice and continuously administrated the drug FTY720 to deplete circulating lymphocytes during the challenge, as described in Fig. 6d, e. We analyzed leukocytes by flow cytometry at day 7 post-infection (Fig. 7) and by histological evaluation of lung sections at day 14 post-infection (Fig. 8). Gates for flow cytometry were set as previously described in a murine cryptococcosis model19 and an asthma model20. At day 7 post-infection, lung leukocytes—including neutrophils, inflammatory monocytes/dendritic cells, and CD4+ TRMs—were significantly increased in the WT mice that had received the DC vaccine. However, the vaccine did not increase lung neutrophils in the IL-17A KO mice. Furthermore, the vaccine induced neutrophils to express Siglec-F and CD11c in an IL-17A-dependent manner (Fig. 7a). Because populations of Siglec-Fhigh Ly6Glow SSChigh eosinophils and alveolar macrophages were detected outside of the neutrophil-gate, neutrophils expressing Siglec-F and CD11c were clearly distinguishable from other cells. Previous studies have shown that a subpopulation of activated neutrophils expressed Siglec-F and CD11c on the cell surface21,22,23,24,25. Siglec-F is known to be an inhibitory receptor that can be expressed in activated lung T cells as well as eosinophils26. Although vascular CD4+ T cells were significantly depleted in this model as shown in Fig. 7c, the number of CD127+ CD4+ TRMs significantly increased in the lungs of the WT and IL-17A-KO mice that had received the DC vaccine, compared with before infection (Fig. 7b). Because CD69 expression significantly increased in the immunized mice, compared with the non-immunized mice, CD127+ CD4+ TRMs might be activated after the infection challenge (Fig. 7b); however, the effector function of CD127+ CD4+ TRMs appeared to be depressed in the IL-17A KO mice. These results suggest that the induction of neutrophils at day 7 post-infection is correlated with the effector function of lung TMR17.
Previous studies suggest that neutrophils are required for the induction of multinucleated giant cells (MGCs) that can suppress the growth of Mycobacterium tuberculosis27. IL-17A-dependent granuloma formation is also known in murine tuberculosis28, cryptococcosis29, and hypersensitivity pneumonitis models25. Our previous study showed that MGC formation was correlated with protective response against pulmonary infection with highly virulent C. gattii7. Therefore, we performed a histological evaluation of lung sections to assess MGC formation and localization of neutrophils at day 14 post-infection (Fig. 8). In these experiments, the WT and IL-17A KO mice were continuously treated by FTY720 to deplete circulating lymphocytes during the challenge, as described in Fig. 6d, e. In the unimmunized mice, many fungal cells were found in destroyed alveolar spaces, but the leukocyte accumulation was limited. The DC vaccine increased leukocytes in the lungs of the WT mice, and induced the formation of MGCs, which engulfed the fungal cells. However, accumulation of leukocytes and MGC formation were significantly lesser in the IL-17A KO mice that received the DC vaccine. Larger alveolar spaces were also observed in the immunized IL-17A KO mice (Fig. 8a). In the lungs stained with hematoxylin–eosin (HE), infiltration of polymorphonuclear leukocytes (PMNs) and lymphocytes was found in the inflammatory region of the immunized mice, and these cells adjacently localized with MGCs (Fig. 8a). Immunohistochemical analysis also showed that considerable Ly6G+ neutrophils adjacently localized with T cells in the lungs of the immunized mice in an IL-17A-dependent manner (Fig. 8b). These results suggest that lung TRM17 is involved in neutrophil recruitments and MGC formation at day 14 post-infection, which might suppress fungal growth in the lungs after infection.
In this study, we demonstrated that a systemic DC vaccine induced lung-resident memory Th17 cells (lung TRM17), which had a characteristic marker profile, antigen-specific memory, and a long life. Our results suggest that lung TRM17 is involved in the induction of neutrophils and MGCs in the lungs after pulmonary infection with the highly virulent C. gattii strain R265. This inflammatory response might be critical for long-term protective effect of the DC vaccine. Our findings provide new insights into an efficient approach for induction of lung-resident memory Th17 cells and a vaccination strategy against pulmonary infection, including the lethal C. gattii infection.
TRM-function of Th1, Th2, and CD8+ cytotoxic lymphocytes has been well-studied. It has been shown that local infection providing cognate antigens was essential to induce TRMs30. On the other hand, few studies investigated memory Th17 cells, and there is even a report indicating that Th17 cells are shorter-lived than Th1 cells31. A recent report showed that intranasal vaccination induced lung-resident memory Th17 cells to promote local IgA responses32. An important distinction in our findings was that systemic vaccination also induced long-lived lung TRM17 with protective effects against pulmonary infection.
To the best of our knowledge, this is the first study showing that TRMs can be induced without local antigen administration or local infection. Our findings raise the question of why the systemic DC vaccine induced TRMs in the lungs rather than in BM or spleen. We showed that antigen-pulsed DCs preferentially accumulated in the lungs after intravenous transfer (Fig. 1). This migration ability might be crucial in inducing TRMs in the lungs. Shimizu et al. demonstrated that intravenous transfer of α-galactosylceramide-loaded DCs induced long-lived invariant natural killer T cells in lungs, and transferred DCs also preferentially migrated to the lungs rather than to the spleen or liver33. There were reports showing that i.v.-injected BM-derived dendritic cells (BMDCs) preferentially migrated to the liver34 or spleen35,36. Such diverse tissue tropism might be attributable to the BMDC induction method. Adaptive-transferred DCs migrated to the liver or spleen rather than to the lungs when IL-4 was used with GM-CSF to cultivate BMDCs34,35. Specific culture conditions possibly allow the expression of specific chemokine receptors controlling DC trafficking. Administration of antigen-loaded DCs has been utilized not only in infection control11 but also in cancer therapy10. Tissue-resident lymphocytes including TRMs and TILs are also involved in ameliorating other infections and cancer, respectively. Thus, the lung-targeting DC vaccine might possess the potential to induce lung TRMs and lung TILs that can control respiratory infections and growing tumors. Future studies are needed to develop ways to regulate organ-specific migration of transferred DCs.
Our findings suggest that MHC-II on donor DCs is partially required for the generation of lung CD4+ TRMs and that recruited endogenous DCs are also involved in the development of lung CD4+ TRMs (Fig. 2c). In the current study, the heat-inactivated fungal cells were used to pulse DCs for preparing the DC vaccine. Because the fungal particles could not be removed from the DC suspensions by centrifugation and washing, the DC vaccine actually comprised BMDC engulfing the fungal cells and the extracellular non-engulfed fungal particles as previously described7. Thus, a considerable number of fungal particles were also injected via the tail vein into the host, while vaccination using heat-inactivated C. gattii strains without DCs had no protective effect7. As shown in Fig. 1, the DC vaccine significantly recruited endogenous DCs; thus, it is likely that the endogenous DCs trapped the intravascular fungal particles for priming CD4+ T cells during the vaccination.
We showed that DC vaccine-mediated lung TRM17 were long-lived in the lungs, until at least 6 months after the immunization (Fig. 4 and Supplementary Figure S2). There have been reports stating that long-lived memory T cells are BCL-2+ and anti-apoptotic37, Ki-67 negative and resting38, autophagy-activated39,40, Wnt/β-catenin pathway-activated41, or dependent on Notch signal-regulated glucose uptake and metabolism42 and lipid-uptake and metabolism43. Lung TRM17 highly expressed Ki-67, TCF-7, stem cell antigen-1 (Sca-1), and the stem cell marker CD43 (Supplementary Figure S3C). TCF-7 is a Wnt signal-mediated transcriptional factor associated with self-renewal of stem cells44 and stem cell-like memory T-cells45. Although it was unknown whether the DC vaccine-mediated lung TRM17 was capable of self-renewal, stem cell-like marker profile might be responsible for the long-lived nature of lung TRM17. Future studies are also needed to elucidate the precise molecular mechanism for the long-lived nature of lung TRM17.
Iijima and Iwasaki showed that vaginal-resident CD4+ TRMs produced a basal level of IFNγ to maintain themselves in the memory lymphocytes cluster (MLC), which establishes a feedback loop with CD11b+ macrophages secreting CCL5. Thus, IFNγ and CCL5 were required for the maintenance of CD4+ TRMs within the MLC46. We showed that IL-17A production was required for the generation and maintenance for lung TRM17 and that vaccine-induced lung TRM17 could produce IL-17A without antigen re-stimulation and expressed IL-17A receptor IL-17RA (Figs. 4 and 5). The amount of lung TRM17 in the IL-17A KO mice decreased to non-vaccinated baseline, and CD127 expression on lung CD4+ TEMs was significantly attenuated in these within 15 weeks of the final vaccination (Fig. 5c). Thus, IL-17A production appears to be required for CD127 expression on lung TRM17. Previous studies have shown that CD127 is a receptor for IL-7 and is responsible for the survival and generation of memory CD4+ T cells47. Future studies are needed to elucidate how paracrine or autocrine IL-17A signals are involved in the generation and maintenance of lung TRM17.
CD4+ TRMs are basically helper cells and probably do not directly kill pathogens or tumor cells. Although the role of IFNγ producing CD4+ TRMs has been often analyzed48, the substantial role of IL-17A-producing CD4+ TRMs has yet to be elucidated. Th1-type skin-resident memory T cells play a role in the induction of inflammatory monocytes after re-infection in a parasite infection model48. On the other hand, intranasal immunization-mediated memory Th17 cells in the lungs promoted local IgA response, but did not show protective effects against any respiratory infections including viral infections32. The DC vaccine-mediated lung TRM17 promptly responded to the antigen re-stimulation and produced IL-17A but not IFNγ (Fig. 4). Furthermore, lung TRM17 appeared to induce neutrophils and MGCs after the infection challenge, which may have contributed to the vaccine-mediated protective effect against highly virulent C. gattii (Figs. 6–8). A Th17-inducing intranasal vaccine also promoted granuloma formation in an IL-17A- and a CXCL13-dependent manner and exhibited a protective effect against M. tuberculosis28. To the best of our knowledge, this is the first study elucidating a substantial role for Th17-type lung-resident memory T cells.
In summary, we demonstrated that a systemic DC vaccine induced long-lived lung-resident memory Th17 cells. A Th17-inducing vaccine is especially awaited in respiratory infections, including tuberculosis, pneumococcal infection, pertussis, and flu6. To further elucidate the precise role of lung TRM17, parabiosis and adoptive transfer experiments of CD4+ TRMs are required in future research as it is currently difficult to specifically deplete TRMs. We believe that the findings of this study will provide important clues for the development of effective vaccines against intractable pulmonary infections.
Materials and methods
C57BL/6J (wild-type, WT) and B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) were purchased from Japan SLC, Inc. (Shizuoka, Japan), and Sankyo Labo Service Corporation (Tokyo, Japan), respectively. The IL-17A KO mice were generated as described previously49. All mice were of the C57BL/6J background. The mice used in this study were sex-matched and age-matched and maintained under specific-pathogen-free conditions at the National Institute of Infectious Diseases of Japan.
The DC vaccine was prepared and injected as previously described7. In brief, BM cells were harvested from the femurs and tibiae of female mice. Red blood cells were lysed with lysis buffer (15 mM ammonium chloride, 1 mM sodium bicarbonate, and 0.1 mM ethylenediaminetetraacetic acid [EDTA]/bisodium, pH 7.4). The BM cells were cultured in RPMI 1640 complete medium that was supplemented with 10% (v/v) fetal bovine serum (FBS), 1% (v/v) streptomycin–penicillin solution (Sigma-Aldrich, St. Louis, MO), 44 μM 2-mercaptoethanol, and 10 ng/mL of mouse granulocyte-macrophage colony-stimulating factor (mGM-CSF, PeproTech, Rocky Hill, NJ) at 37 °C under 5% CO2. On days 3 and 5, medium was changed to maintain the BM cells. On day 6, non-adherent cells were collected and used as BMDCs. BMDCs were incubated in RPMI 1640 complete medium with heat-killed C. gattii CAP60∆ (MOI = 5) for 24 h in petri dishes. After incubation, non-adherent cells were collected and washed twice with phosphate buffered saline (PBS). BMDCs pulsed with heat-killed C. gattii CAP60∆ (5 × 105 cells/mouse) were injected twice every 2 weeks via the tail vein. For the blocking of MHC-II, antigen-pulsed DCs were treated with 10 µg/mL anti-I-A/I-E mAb (M15/114.15.2, BioLegend, San Diego, CA) or rat IgG2b isotype control antibody (RTK4530, BioLegend, San Diego, CA) and washed twice with PBS.
FTY720 treatment and intravascular staining
FTY720 (Cayman Chemical, Ann Arbor, MI) was dissolved in dimethyl sulfoxide (DMSO) to prepare a 50 mg/mL stock solution. The stock solution was diluted with saline and distilled water for intraperitoneal administration (0.5–1.0 mg/kg) and administration in drinking water (2.5 mg/L), respectively. To stain vascular leukocytes (IV staining), 2.5 μg of FITC-labeled anti-CD45 mAb (BioLegend, San Diego, CA) was injected into the orbital vein under isoflurane anesthesia 5 min prior to euthanasia.
Leukocytes in the BM, spleen, lungs, and MedLN were prepared as previously described7. In brief, the BM, spleen, and MedLN were mashed on a 70-μm cell strainer, and red blood cells were then lysed with the lysis buffer, as described above. The lungs were minced and digested with collagenase-D, hyaluronidase and DNase I (Sigma-Aldrich, St. Louis, MO). Lung leukocytes were enriched with Percoll gradient (30%, 44%, and 70%) centrifugation.
Flow cytometry was performed as previously described7. Antibodies used in this study are listed in Supplementary Table S1. Staining buffer was PBS containing 2 mM EDTA, 0.5% (w/v) bovine serum albumin, and 0.1% (w/v) sodium azide. Fixation and permeabilization buffers were purchased from BioLegend (San Diego, CA). FOXP3/transcription factor staining buffer set (eBioscience, San Diego, CA) was used to stain transcription factors. Gating strategies used for lung leukocytes19,20 and memory T cells13 were as previously described. Data were acquired with a BD FACSCalibur or BD FACSCantoII flow cytometer (BD Bioscience, San Jose, CA) and analyzed using FlowJo software (TreeStar Inc., Ashland, OR).
T-cell re-stimulation assay
For polyclonal re-stimulation, the lung, spleen, and BM leukocytes were incubated in RPMI 1640 medium with 50 ng/mL of PMA and 1 μM ionomycin for 4 h. Alternatively, for the antigen-specific re-stimulation, BMDCs (5 × 105 cells/well) were pulsed with heat-killed C. gattii CAP60∆ (5 × 105 cells/well) overnight. After the culture supernatant was removed, antigen-pulsed BMDCs were co-cultivated with lung leukocytes or spleen leukocytes (1 × 106 cells/well) for 18 h to stimulate T-cell response. In each experiment, cells were treated with 5 μg/mL brefeldin A and 2 μM monensin for the last 2 h of culture to stop cytokine release. Intracellular cytokines in T cells were evaluated using flow cytometry. In another experiment, lung and spleen leukocytes (1 × 106 cells/well) were cultivated for 20 h with heat-killed C. albicans SC5314 (MOI = 1), C. gattii CAP60∆ (MOI = 1), or C. gattii R265 (MOI = 10). After cultivation, IL-17A in culture supernatant was measured using enzyme-linked immunosorbent assay (ELISA).
Supernatants of cell culture or lung homogenates were collected and cytokine levels were determined using ELISA. MaxiSorp plates (Thermo Fisher Scientific, Roskilde, Denmark) and a DuoSet ELISA kit (R&D Systems, Minneapolis, MN) or BD OptEIA ELISA sets (BD Bioscience, San Jose, CA) were used as per the manufacturers’ instructions.
Intratracheal infection was performed as previously described7. In brief, C. gattii R265 was cultured in YPD at 30 °C with shaking overnight. Then the cells were washed and resuspended in PBS. The mice were anesthetized with isoflurane, after which a fungal suspension (3 × 103 CFU) was intratracheally injected using an indwelling needle. They were euthanized with carbon dioxide inhalation to harvest their lungs, and the lungs were homogenized using a stainless-steel mesh in PBS. To determine the fungal burden in lungs, lung homogenates were diluted and spread onto YPD plates. These plates were incubated at 30 °C for 24 h, after which colonies were counted.
The lungs were fixed in 10% formalin, dehydrated, and embedded in paraffin. Paraffin blocks were cut into 4-μm sections and stained with HE or Verhoeff’s elastin staining and periodic acid-Schiff’s method (EPAS). MGCs were examined to assess macrophage recruitment into the lungs after infection. Numbers of MGCs per unit area (square millimeters) were determined as previously described7. Frozen lung sections were prepared and stained as previously described50. Antibodies used in this experiment are also listed in Supplementary Table S1. Infected lungs were fixed in fixation buffer, 2% (w/v)‐paraformaldehyde (PFA) in 50 mM phosphate buffer, for 6 h at 4 °C prior to be embedded in Tissue-Tek OCT compound (Sakura Finetek Inc., Torrance, CA). Fluorescent-labeled lung sections were observed under a confocal laser-scanning microscope LSM 700 (Carl Zeiss, Oberkochen, Germany), and the images were analyzed by ZEN software (Carl Zeiss, Oberkochen, Germany).
GraphPad Prism 7 (GraphPad Software, Inc., La Jolla, CA) was used for all statistical analyses. P-values of less than 0.05 were considered statistically significant.
Mueller, S. N. & Mackay, L. K. Tissue-resident memory T cells: local specialists in immune defence. Nat. Rev. Immunol. 16, 79–89 (2016).
Park, C. O. & Kupper, T. S. The emerging role of resident memory T cells in protective immunity and inflammatory disease. Nat. Med. 21, 688–697 (2015).
Ganesan, A.-P. et al. Tissue-resident memory features are linked to the magnitude of cytotoxic T cell responses in human lung cancer. Nat. Immunol. 18, 940–950 (2017).
Hayashizaki, K. et al. Myosin light chains 9 and 12 are functional ligands for CD69 that regulate airway inflammation. Sci. Immunol. 1, eaaf9154 (2016).
Mackay, L. K. et al. Cutting edge: CD69 interference with sphingosine-1-phosphate receptor function regulates peripheral T cell retention. J. Immunol. 194, 2059–2063 (2015).
Lin, Y., Slight, S. R. & Khader, S. A. Th17 cytokines and vaccine-induced immunity. Semin. Immunopathol. 32, 79–90 (2010).
Ueno, K. et al. Dendritic cell-based immunization ameliorates pulmonary infection with highly virulent Cryptococcus gattii. Infect. Immun. 83, 1577–1586 (2015).
Lizarazo, J. et al. Retrospective study of the epidemiology and clinical manifestations of Cryptococcus gattii infections in Colombia from 1997–2011. PLoS Negl. Trop. Dis. 8, e3272 (2014).
CDC Emergence of Cryptococcus gattii, Pacific Northwest, 2004–2010. Morb. Mortal. Wkly. Rep. 59, 865–868 (2010).
Palucka, K. & Banchereau, J. Dendritic-cell-based therapeutic cancer vaccines. Immunity 39, 38–48 (2013).
García, F. et al. A therapeutic dendritic cell-based vaccine for HIV-1 infection. J. Infect. Dis. 203, 473–478 (2011).
Roy, R. M. & Klein, B. S. Dendritic cells in antifungal immunity and vaccine design. Cell Host Microbe 11, 436–446 (2012).
Turner, D. L. & Farber, D. L. Mucosal resident memory CD4 T cells in protection and immunopathology. Front. Immunol. 5, 331 (2014).
McKinstry, K. K. et al. Effector CD4 T-cell transition to memory requires late cognate interactions that induce autocrine IL-2. Nat. Commun. 5, 5377 (2014).
Takenaka, M. et al. Antibodies to MHC class II molecules induce autoimmunity: critical role for macrophages in the immunopathogenesis of obliterative airway disease. PLoS ONE 7, e42370 (2012).
Anderson, K. G. et al. Intravascular staining for discrimination of vascular and tissue leukocytes. Nat. Protoc. 9, 209–222 (2014).
Turner, D. L. et al. Lung niches for the generation and maintenance of tissue-resident memory T cells. Mucosal Immunol. 7, 501–510 (2014).
Laidlaw, B. J. et al. CD4(+) T cell help guides formation of CD103(+) lung-resident memory CD8(+) T cells during influenza viral infection. Immunity 41, 633–645 (2014).
Sionov, E. et al. Type I IFN induction via poly-ICLC protects mice against Cryptococcosis. PLoS Pathog. 11, e1005040 (2015).
Iwata, A. et al. Th2-type inflammation instructs inflammatory dendritic cells to induce airway hyperreactivity. Int. Immunol. 26, 103–114 (2013).
Lewis, S. M. et al. Expression of CD11c and EMR2 on neutrophils: potential diagnostic biomarkers for sepsis and systemic inflammation. Clin. Exp. Immunol. 182, 184–194 (2015).
Villa-Ambriz, J., Rodríguez-Orozco, A. R., Béjar-Lozano, C. & Cortés-Rojo, C. The increased expression of CD11c and CD103 molecules in the neutrophils of the peripheral blood treated with a formula of bacterial ribosomes and proteoglycans of Klebsiella pneumoniae. Arch. Bronconeumol. 48, 316–319 (2012).
Percopo, C. M. et al. SiglecF+Gr1hi eosinophils are a distinct subpopulation within the lungs of allergen-challenged mice. J. Leukoc. Biol. 101, 321–328 (2017).
Kiwamoto, T., Kawasaki, N., Paulson, J. C. & Bochner, B. S. Siglec-8 as a drugable target to treat eosinophil and mast cell-associated conditions. Pharmacol. Ther. 135, 327–336 (2012).
Ishizuka, M. et al. Interleukin-17A and neutrophils in a murine model of bird-related hypersensitivity pneumonitis. PLoS ONE 10, e0137978 (2015).
Zhang, M. et al. Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils. Blood 109, 4280–4287 (2007).
Yoshioka, Y. et al. Neutrophils and the S100A9 protein critically regulate granuloma formation. Blood Adv. 1, 184–192 (2016).
Gopal, R. et al. Interleukin-17-dependent CXCL-13 mediates mucosal vaccine-induced immunity against tuberculosis. Mucosal Immunol. 6, 972–984 (2013).
Murdock, B. J., Huffnagle, G. B., Olszewski, M. A. & Osterholzer, J. J. Interleukin-17A enhances host defense against cryptococcal lung infection through effects mediated by leukocyte recruitment, activation, and gamma interferon production. Infect. Immun. 82, 937–948 (2014).
Khan, T. N., Mooster, J. L., Kilgore, A. M., Osborn, J. F. & Nolz, J. C. Local antigen in nonlymphoid tissue promotes resident memory CD8+ T cell formation during viral infection. J. Exp. Med. 213, 951–966 (2016).
Pepper, M. et al. Different routes of bacterial infection induce long-lived TH1 memory cells and short-lived TH17 cells. Nat. Immunol. 11, 83–89 (2009).
Christensen, D., Mortensen, R., Rosenkrands, I., Dietrich, J. & Andersen, P. Vaccine-induced Th17 cells are established as resident memory cells in the lung and promote local IgA responses. Mucosal Immunol. 10, 260–270 (2017).
Shimizu, K. et al. KLRG+ invariant natural killer T cells are long-lived effectors. Proc. Natl. Acad. Sci. USA 111, 12474–12479 (2014).
Lappin, M. B. et al. Analysis of mouse dendritic cell migration in vivo upon subcutaneous and intravenous injection. Immunology 98, 181–188 (1999).
Mullins, D. W. et al. Route of immunization with peptide-pulsed dendritic cells controls the distribution of memory and effector T cells in lymphoid tissues and determines the pattern of regional tumor control. J. Exp. Med. 198, 1023–1034 (2003).
Sheasley-O’Neill, S. L., Brinkman, C. C., Ferguson, A. R., Dispenza, M. C. & Engelhard, V. H. Dendritic cell immunization route determines integrin expression and lymphoid and nonlymphoid tissue distribution of CD8 T cells. J. Immunol. 178, 1512–1522 (2007).
Kryczek, I. et al. Human TH17 cells are long-lived effector memory cells. Sci. Transl. Med. 3, 104ra100 (2011).
Sercan Alp, Ö. et al. Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription. Eur. J. Immunol. 45, 975–987 (2015).
Xu, X. et al. Autophagy is essential for effector CD8(+) T cell survival and memory formation. Nat. Immunol. 15, 1152–1161 (2014).
Araki, K. et al. mTOR regulates memory CD8 T-cell differentiation. Nature 460, 108–112 (2009).
Muranski, P. et al. Th17 cells are long lived and retain a stem cell-like molecular signature. Immunity 35, 972–985 (2011).
Maekawa, Y. et al. Notch controls the survival of memory CD4+ T cells by regulating glucose uptake. Nat. Med. 21, 55–61 (2015).
Pan, Y. et al. Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism. Nature 543, 252–256 (2017).
Reya, T. et al. A role for Wnt signalling in self-renewal of haematopoietic stem cells. Nature 423, 409–414 (2003).
Gattinoni, L. et al. Wnt signaling arrests effector T cell differentiation and generates CD8+ memory stem cells. Nat. Med. 15, 808–813 (2009).
Iijima, N. & Iwasaki, A. T cell memory. A local macrophage chemokine network sustains protective tissue-resident memory CD4 T cells. Science 346, 93–98 (2014).
Kondrack, R. M. et al. Interleukin 7 regulates the survival and generation of memory CD4 cells. J. Exp. Med. 198, 1797–1806 (2003).
Glennie, N. D., Volk, S. W. & Scott, P. Skin-resident CD4+ T cells protect against Leishmania major by recruiting and activating inflammatory monocytes. PLoS Pathog. 13, e1006349 (2017).
Nakae, S. et al. Antigen-specific T cell sensitization is impaired in IL-17-deficient mice, causing suppression of allergic cellular and humoral responses. Immunity 17, 375–387 (2002).
Adachi, Y. et al. Distinct germinal center selection at local sites shapes memory B cell response to viral escape. J. Exp. Med. 212, 1709–1723 (2015).
This work was supported by KAKENHI (15K21644, 16H05349, and 17K18385) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, by grants from the Japan Agency for Medical Research and Development, AMED, by the Takeda Science Foundation, and by the LEGEND Research Grant Program 2015 from Tomy Digital Biology Co. Ltd. We also thank Drs. Yu Adachi, Taishi Onodera, Yoshimasa Takahashi, and Koji Hayashizaki who provided the technical advice for immunohistochemical analysis.
The authors declare no competing interests.
All animal experiment protocols were approved by the Ethical Committee of the National Institute of Infectious Diseases, Japan (approval numbers 115015, 116019, 116124, 215031, 215036, 215047, 114029, 117032, and 114029) and were performed in accordance with the approved guidelines and regulations.
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Ueno, K., Urai, M., Sadamoto, S. et al. A dendritic cell-based systemic vaccine induces long-lived lung-resident memory Th17 cells and ameliorates pulmonary mycosis. Mucosal Immunol 12, 265–276 (2019). https://doi.org/10.1038/s41385-018-0094-4
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