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Translational profiling of mouse dopaminoceptive neurons reveals region-specific gene expression, exon usage, and striatal prostaglandin E2 modulatory effects

Molecular Psychiatry volume 27, pages 2068–2079 (2022)Cite this article

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Abstract

Forebrain dopamine-sensitive (dopaminoceptive) neurons play a key role in movement, action selection, motivation, and working memory. Their activity is altered in Parkinson’s disease, addiction, schizophrenia, and other conditions, and drugs that stimulate or antagonize dopamine receptors have major therapeutic applications. Yet, similarities and differences between the various neuronal populations sensitive to dopamine have not been systematically explored. To characterize them, we compared translating mRNAs in the dorsal striatum and nucleus accumbens neurons expressing D1 or D2 dopamine receptor and prefrontal cortex neurons expressing D1 receptor. We identified genome-wide cortico-striatal, striatal D1/D2 and dorso/ventral differences in the translating mRNA and isoform landscapes, which characterize dopaminoceptive neuronal populations. Expression patterns and network analyses identified novel transcription factors with presumptive roles in these differences. Prostaglandin E2 (PGE2) was a candidate upstream regulator in the dorsal striatum. We pharmacologically explored this hypothesis and showed that misoprostol, a PGE2 receptor agonist, decreased the excitability of D2 striatal projection neurons in slices, and diminished their activity in vivo during novel environment exploration. We found that misoprostol also modulates mouse behavior including by facilitating reversal learning. Our study provides powerful resources for characterizing dopamine target neurons, new information about striatal gene expression patterns and regulation. It also reveals the unforeseen role of PGE2 in the striatum as a potential neuromodulator and an attractive therapeutic target.

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Fig. 1: EGFP-L10a expression and differences in ribosome-associated mRNA expression in the PFC and striatum of D1-TRAP mice.
Fig. 2: Differential ribosome-associated mRNA expression in striatal regions of D1- and D2-TRAP mice.
Fig. 3: Expression of PGE2 receptors in the striatum and cell population-specific effects of PGE2 receptor stimulation.
Fig. 4: Effects of PGE2 receptor stimulation on electrophysiological properties of DS D1-SPNs and D2-SPNs.
Fig. 5: Effects of PGE2 receptor stimulation on DS neuron activity and mouse behavior.

Data availability

Sequencing data have been deposited in NCBI’s Gene Expression Omnibus and are accessible through GEO Series accession number GSE137153.

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Acknowledgements

The paper is dedicated to the memory of PG who passed away on April 13th, 2019, before the paper was completed. Authors thank the Babraham Institute’s Bioinformatics team for help with read mapping and counting, Vincent Knight-Shrijver for his volcano-plot R script, and Lucile Marion-Poll for helpful suggestions.

Funding

This work was supported by Inserm and Sorbonne Université, and grants from European Research Council (ERC, AdG-250349) and Biology for Psychiatry Laboratory of excellence (Labex Bio-Psy, Investissements d’Avenir, ANR-11-IDEX-0004-02) to JAG, Fondation pour la Recherche Médicale (FRM # DPA20140629798) and ANR (Epitraces, ANR-16-CE16-0018) to JAG and EV, ANR-17-CE37-0007 (Metacognition) to CM, the United States Army Medical Research and Material Command (USAMRMC) Award No. W81XWH-14-1-0046 to JPR, the Fisher Center for Alzheimer’s Disease Research to JPR and PG, NIH grants DA018343 and DA040454 to ACN. EM was supported by a Marie Curie International Training Network (ITN) N-PLAST. AG is a Ramón y Cajal fellow (RYC-2016-19466) and is supported by a grant from the Spain Ministerio de Ciencia, Innovación y Universidades (Project no. RTI2018-094678-A-I00). LC was supported by a Labex EpiGenMed PhD fellowship (Investissements d’avenir, ANR-10-LABX-12-01). YN was recipient of Uehara Memorial Foundation and Fyssen Foundation fellowships. BdP was supported by FRM (FDT201805005390). NG was supported by BBSRC (BB/P013406/1, BB/P013414/1, BB/P013384/1). KDN received an Amgen Scholarship. LV received support from the Erasmus + program. Work in FJM lab was supported by the ERC under the European Union’s Horizon 2020 research and innovation program (grant agreement 804089; ReCoDE) and the NWO Gravitation project BRAINSCAPES: A Roadmap from Neurogenetics to Neurobiology (024.004.012).

Author information

Author notes

  1. Laia Castell

    Present address: Department of Psychological and Brain Sciences, Krieger School of Arts and Sciences, Johns Hopkins University, Baltimore, MD, USA

  2. Katrina Daila Neiburga

    Present address: Bioinformatics Lab, Riga Stradins University, Riga, Latvia

  3. Benoit de Pins

    Present address: Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel

  4. Albert Giralt

    Present address: Departament de Biomedicina, Facultat de Medicina, Institut de Neurociències, Universitat de Barcelona, Barcelona, Spain

  5. Albert Giralt

    Present address: Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, Madrid, Spain

  6. Albert Giralt

    Present address: Institut d’Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain

  7. Albert Giralt

    Present address: Production and Validation Center of Advanced Therapies (Creatio), University of Barcelona, Barcelona, Spain

  8. Lieng Taing

    Present address: UMR1166, Faculté de Médecine, Sorbonne University, Paris, France

  9. Letizia Vestito

    Present address: University College London, London, UK

  10. Wei Wang

    Present address: Bioinformatics Resource Center, Rockefeller University, New York, NY, USA

  11. Jean-Pierre Roussarie

    Present address: Boston University School of Medicine, Department of Anatomy & Neurobiology, Boston, MA, USA

Authors and Affiliations

  1. Inserm UMR-S 1270, Paris, France

    Enrica Montalban, Albert Giralt, Lieng Taing, Yuki Nakamura, Benoit de Pins, Assunta Pelosi, Laurence Goutebroze, Denis Hervé & Jean-Antoine Girault

  2. Faculty of Sciences and Engineering, Sorbonne Université, Paris, France

    Enrica Montalban, Albert Giralt, Lieng Taing, Yuki Nakamura, Benoit de Pins, Assunta Pelosi, Laurence Goutebroze, Denis Hervé & Jean-Antoine Girault

  3. Institut du Fer à Moulin, Paris, France

    Enrica Montalban, Albert Giralt, Lieng Taing, Yuki Nakamura, Benoit de Pins, Assunta Pelosi, Laurence Goutebroze, Denis Hervé & Jean-Antoine Girault

  4. Université de Paris, CNRS, Unité de Biologie Fonctionnelle et Adaptative, Paris, France

    Enrica Montalban, Julien Castel, Serge Luquet & Claire Martin

  5. Department of Translational Neuroscience, Brain Center, UMC Utrecht, Utrecht University, Utrecht, The Netherlands

    Evelien H. S. Schut, Laura F. Supiot & Frank J. Meye

  6. IGF, CNRS, INSERM, University of Montpellier, Montpellier, France

    Laia Castell, Pola Tuduri & Emmanuel Valjent

  7. Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY, USA

    Wei Wang, Paul Greengard & Jean-Pierre Roussarie

  8. Babraham Institute, Cambridge, UK

    Katrina Daila Neiburga & Letizia Vestito

  9. Department of Psychiatry, Yale School of Medicine, Connecticut Mental Health Center, New Haven, CT, USA

    Angus C. Nairn

  10. Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA

    Nathaniel Heintz

  11. aSciStance Ltd, Great Chesterford, UK

    Nicolas Gambardella

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  1. Enrica Montalban
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Contributions

JAG and JPR conceived and supervised the project. AG, CM, DH, EM, EV, FJM, JAG, JPR, LG, and NG designed the experiments. AG, AP, BdP, CM, EHSS, EM, EV, JPR, LC, LG, JC, LFS, PT, FJM, and YN performed experiments. ACN, AG, CM, DH, EM, EV, FJM, LG, JAG, and JPR analyzed data, JPR, KDN, LT, LV, NG, and WW performed and interpreted bioinformatics analyses, ACN, AG, AP, BdP, CM, DH, EM, EV, FJM, JAG, LG, LT, NG, NH, PG, SL, and YN discussed the data and provided input and corrections to the paper. EM, JPR, and JAG wrote the paper. All the authors but PG approved the final version of the paper.

Corresponding authors

Correspondence to Jean-Pierre Roussarie or Jean-Antoine Girault.

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Competing interests

The authors declare no competing interests.

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Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Supplementary Methods

Supplementary Figures

List of Supplementary Tables

R script used for network inference

Supplementary Tables 1

Supplementary Table 2

Supplementary Table 3: DE-genes_PFC-vs-striatum

Supplementary Table 4: DEXSeq_PFC-vs-Str_in-D1

Supplementary Table 5: DEXSeq_PFC-vs-Str_in-D1_Analyses

Supplementary Table 6: DE-genes_D1-vs-D2_in-Str

Supplementary Table 7: DE-genes_D1-vs-D2_in-STR_GO-IUPHAR

Supplementary Table 8: DEXSeq_D1-vs-D2_in-DS

Supplementary Table 9: DEXSeq_D1-vs-D2_in-NAc

Supplementary Table 10: DEXSeq_D1-vs-D2_in-DS-and-NAc_Analyses

Supplementary Table 11: DE-genes_DS-vs-NAc

Supplementary Table 12: DE-genes_DS-vs-NAc_GO-IUPHAR

Supplementary Table 13: DEXSeq_DS-vs-NAc_in-D1

Supplementary Table 14: DEXSeq_DS-vs-NAc_in-D2

Supplementary Table 15: DEXSeq_DS-vs-NAc_in-D1-and-D2_Analyses

Supplementary Table 16: DE-Transcription-factors_STR

Supplementary Table 17: IPA-upstream-analysis_DS-vs-NAc

Supplementary Table 18: Prostaglandin-related-gene products

Supplementary Table 19: Statistical analyses

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Montalban, E., Giralt, A., Taing, L. et al. Translational profiling of mouse dopaminoceptive neurons reveals region-specific gene expression, exon usage, and striatal prostaglandin E2 modulatory effects. Mol Psychiatry 27, 2068–2079 (2022). https://doi.org/10.1038/s41380-022-01439-4

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