Fig. 5 | Molecular Psychiatry

Fig. 5

From: Emergence of 5-HT5A signaling in parvalbumin neurons mediates delayed antidepressant action

Fig. 5

Activating cAMP signaling in PV DG cells attenuates the response to chronic fluoxetine. a Chemogenetic manipulation of PV DG cells consisted of bilateral injection of 1 μl rAAV2/hsyn-DIO- expressing one of three constructs: Gs-DREADD, Gi-DREADD or mCherry. b Immunofluorescence image depicting the expression of mCherry following Gs-DREADD delivery. Scale bar: 200 μm. c Immunolabeling confirmed that all mCherry immunopositive cells co-expressed PV. Scale bar: 50 μm. d Representative traces of patch-clamp recordings in hippocampal slices from mice in which PV neurons were infected with Gs-DREADD or Gi-DREADD. The right panel shows the hyperpolarization/depolarization of the membrane potential induced by the application of 2 µM CNO on the PV neurons infected with Gi-DREADD (−6.13 ± 2.4 mV n = 4, 3) or Gs-DREADD (9.03 ± 4.4, n = 3, 3). e Fluoxetine was administered for 18 days in the drinking water after which a single dose of CNO (4 mg/kg, intraperitoneally) was injected 30 min before the test. f Immobility time in the FST in PV-Cre mice injected with mCherry (n = 10 mice), Gi-DREADD (n = 10 mice) or Gs-DREADD (n = 6 mice). One-way ANOVA F [2,24] = 6.22, P = 0.007. *P = 0.023 by post hoc Dunnet

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