Fig. 4 | Molecular Psychiatry

Fig. 4

From: Emergence of 5-HT5A signaling in parvalbumin neurons mediates delayed antidepressant action

Fig. 4

5-HT5AR mediates behavioral response to chronic SSRIs. a Immobility time in the forced swim test (FST) after drinking saccharine alone (Veh) or fluoxetine/saccharine mixture (Flx) for 18 days in WT or 5-HT5A cKO. Two-way ANOVA, F [1,44] = 4.58, P = 0.038. b Immobility time in the tail suspension test (TST) after Veh or Flx (for 18 days) in WT or cKO. Two-way ANOVA, F [1,42] = 13.83, P = 0.006. c Latency to approach food pellet in the novelty suppressed feeding (NSF) after Veh or Flx (for 18 days) in WT or 5- cKO. Two-way ANOVA, F [1,41] = 18.58, P < 0.0001. d Immobility time in FST 15 min after a single saline (Sal) or fluoxetine (Flx) intraperitoneal injection in WT or cKO. Two-way ANOVA, F genotype X treatment [1,34] = 3.99, P = 0.07; F treatment [1,34] = 37.27, P < 0.0001. e Immobility time in TST 15 min after a single injection of Sal or Flx in WT or cKO. Two-way ANOVA, F genotype X treatment [1,24] = 0.30, P = 0.590; F treatment [1,24] = 17.47, P = 0.0003; F genotype [1,24] = 17.93, P = 0.0003. *P < 0.05, **P < 0.01 ***P < 0.001 by post hoc Bonferroni. f Study design of viral mediated 5-HT5A gene delivery to PV-Cre and 5-HT5A cKO mice. g GFP-positive cells in the SGZ and hilus but not in the CA1 or outside the hippocampus after AAV.Flex-GFP injection to the DG. Scale bar: 100 μm. h GFP in a SGZ PV+ neuron. Scale bar: 50 μm. i, j Colocalization of 5-HT5A in PV neurons after injection of AAV.Flex-GFP (i) or AAV.Flex-5-HT5A (j) to 5-HT5A cKO. Numbers represent the fraction of SGZ PV+ cells immunopositive for 5-HT5A. Scale bars: 50 μm. k Immobility time in the FST after 18-day fluoxetine treatment in PV-Cre (control) injected with AAV.Flex-GFP or AAV.Flex-5-HT5A or in cKO. Two-way ANOVA, F genotype X AAV [1,42] = 4.54, P = 0.039. l Immobility time in TST. Two-way ANOVA, F genotype X AAV [1,44] = 2.37, P = 0.138; F genotype [1,44] = 60.62, P < 0.0001; F AAV [1,44] = 12, P = 0.0012. m Latency in NSF. Two-way ANOVA, F genotype X AAV [1,33] = 5.75, P = 0.022. *P < 0.05, **P < 0.01 by post hoc multiple t-tests with FDR

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