Fig. 5: Occurrence of a knob-like membrane protrusion at the bacterial poles and increase in the cell length upon drug treatment in the microfluidic device. | Microsystems & Nanoengineering

Fig. 5: Occurrence of a knob-like membrane protrusion at the bacterial poles and increase in the cell length upon drug treatment in the microfluidic device.

From: Development of single-cell-level microfluidic technology for long-term growth visualization of living cultures of Mycobacterium smegmatis

Fig. 5

Bright-field images of live M. smegmatis cells (a) cultured in 7H9-ADC for 16 h compared to (b) those cultured in CB7620870 for 29 h. In the drug treatment group, cells were allowed to grow for 7 h in 7H9-ADC media first and then treated with 200 µM drug for 29 h. Drug-treated cells showed clear knob-shaped swelling at the poles (red arrows). Microscopy images showing the M. smegmatis cell length change when (c) grown in 7H9-ADC for 6 h and when (d) exposed to CB7620870 for 29 h, displaying an elongated shape after treatment. e Histogram of M. smegmatis cell length changes after exposure to the drug CB7620870 (9.2 ± 4.9 μm) and when cultured in normal media 7H9-ADC (4.3 ± 1.9 μm). Scale bar = 5 µm.

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