Fig. 4: Comparison of the growth dynamics of M. smegmatis in the microfluidic device. | Microsystems & Nanoengineering

Fig. 4: Comparison of the growth dynamics of M. smegmatis in the microfluidic device.

From: Development of single-cell-level microfluidic technology for long-term growth visualization of living cultures of Mycobacterium smegmatis

Fig. 4

Single-cell growth inside the multichannel microfluidic device was observed for 18 h, and images were obtained hourly. M. smegmatis cells were loaded into 3 parallel microchannels and allowed to grow for 7 h undisturbed. At this time point (t = 7 h, denoted by the red asterisk in the graph), no drug was added to channel 1, but CB7620870 was added to channel 2 at a 50 μM concentration and to channel 3 at a 200 μM concentration. There was an ~2–3 h delay in the observed changes in the growth rate (comparing the cell numbers of drug-treated cells [squares and triangles] to those of untreated cells [circles]).

Back to article page